Sterility Monitoring of Canine Packed Red Blood Cells

Abstract

A constant risk factor of transfusion is microbial contamina on of whole blood and its components. Because using contaminated blood products can lead to sepsis and high risks to the health of recipients. Blood can be a good nutrient medium for microorganisms, so the risk of bacteria growth in any blood component a er it has been donated is significant. Viola on in the rules of asepsis during blood dona on, processing of blood products, damage to blood collec on systems or their ghtness, etc. can cause microbial contamina on.We examined 5 samples of preserved canine packed red blood cells. The donor animals were 5 clinically healthy dogs. Blood was collected in closed systems with a CPDA an coagulant. A er following centrifuging, plasma was separated from red blood cells in different containers. The remaining packed red blood cells were stored at a temperature of +2-6°C for 30 days.The bacteria cul va on method is considered the "gold standard" for assessing the presence of microbial contamina on in most blood transfusion centers. The tested canine packed red blood cells samples were inoculated into thioglycolate and Sabouraud media and incubated in a thermostat at +20-25 °C. The incuba on period was 14 days. According to the results of the bacteriological examina on of these samples of canine packed red blood cells a er their storage, a non-sterile sample was no found.Thereby, the method of blood collec on using closed systems with CPDA an coagulant is reliable and allows obtaining donor blood and its components without loss of sterility during long-term storage.