Sterilization by Means of Ozone

Ingram, M.; Barnes, Ella M.

doi:10.1111/j.1365-2672.1954.tb02050.x

Cited by 14 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results suggest that sensitivity to ozone is dependent on the stage of cell growth. A different methodology used by Ingram and Barnes (1954) and Foegeding (1986) also found differences in sensitivity of cells at different stages of growth to ozone treatments. It has also been demonstrated that sensitivity of bacteria to various treatments is dependent upon other environmental factors, on the type of organism and the stage of cellular growth (Mackey 1984).…”

Section: Resultsmentioning

confidence: 96%

Sensorial and microbial effects of gaseous ozone on fresh scad (Trachurus trachurus)

1998

View full text Add to dashboard Cite

The bactericidal activity of gaseous ozone was investigated using a commercial ozone generator. Five species of fish bacteria, Pseudomonas putida, Shewanella putrefaciens, Brochothrix thermosphacta, Enterobacter sp. and Lactobacillus plantarum, were inoculated on agar surfaces and exposed to different ozonation times in a gas chamber. Results showed ozone in relatively low concentrations (< 0.27 x 10(-3) g l-1) was an effective bactericide of vegetative cells of the five fish bacteria. The age of the cell culture was shown to influence the cell response following exposure. Survival rate was not linearly related to ozonation time, but exhibited biphasic death over an extended period. Similar bactericidal effects were observed on fish skin treated with ozone daily in the laboratory, with decreases of 1.0 log cfu cm-2 for the micro-organisms studied. Whole fish treated daily in the laboratory using a commercial ozone generator showed improved scores for sensory analyses compared with the controls. The results were statistically significant. Fish treated on board ships were also analysed for microbiological and sensory changes. Controls were obtained from a similar vessel without the ozone facility in the hold. Similar trends to those recorded in the laboratory for the microbiological and sensory results on ozonated fish were observed.

show abstract

Section: Resultsmentioning

confidence: 96%

Sensorial and microbial effects of gaseous ozone on fresh scad (Trachurus trachurus)

1998

View full text Add to dashboard Cite

show abstract

“…The sensitivity of microorganisms to ozone is profoundly affected by the organic nature of the medium, with protection caused both by physical factors, as in the case of agar, and by reduced ozone levels due to ozone demand of organic nutrients in the medium or in environmental waters (2,8,15,16). This study shows that the type of organic material present during ozonation is more important than the amount present.…”

mentioning

confidence: 77%

Efficacy of ozonated water against various food-related microorganisms

Restaino¹,

Frampton²,

Hemphill³

et al. 1995

Appl Environ Microbiol

334

110

View full text Add to dashboard Cite

The antimicrobial effects of ozonated water in a recirculating concurrent reactor were evaluated against four gram-positive and four gram-negative bacteria, two yeasts, and spores of Aspergillus niger. More than 5 log units each of Salmonella typhimurium and Escherichia coli cells were killed instantaneously in ozonated water with or without addition of 20 ppm of soluble starch (SS). In ozonated water, death rates among the gram-negative bacteria-S. typhimurium, E. coli, Pseudomonas aeruginosa, and Yersinia enterocolitica-were not significantly different (P > 0.05). Among gram-positive bacteria, Listeria monocytogenes was significantly P < 0.05) more sensitive than either Staphylococcus aureus or Enterococcus faecalis. In the presence of organic material, death rates of S. aureus compared with L. monocytogenes and E. coli compared with S. typhimurium in ozonated water were not significantly (P > 0.05) affected by SS addition but were significantly reduced (P < 0.05) by addition of 20 ppm of bovine serum albumin (BSA). More than 4.5 log units each of Candida albicans and Zygosaccharomyces bailii cells were killed instantaneously in ozonated water, whereas less than 1 log unit of Aspergillus niger spores was killed after a 5-min exposure. The average ozone output levels in the deionized water (0.188 mg/ml) or water with SS (0.198 mg/ml) did not differ significantly (P < 0.05) but were significantly lower in water containing BSA (0.149 mg/ml). ''The treatment of domestic water to provide a microbiologically safe, aesthetic, potable end product has been normal practice since before the turn of the twentieth century'' (5). Chlorine at low concentrations has been the usual agent of choice in drinking-water purification. At the low concentrations of chlorine used, however, its limitations as a bactericide (3, 4) as well as limitations in its effectiveness against certain eukaryotic pathogens and viruses (18, 20, 21, 25, 27) have been recognized. Furthermore, chlorine at low concentrations may alter certain organic compounds in water producing off tastes and odors as well as forming chloro-organic compounds with carcinogenic potential (22). As an alternative to chlorination in drinking-water disinfection, ozonation of water supplies, which was done first a century ago (29), has become an established means of disinfection and has been reviewed by Rice et al. (25). Bacteria, including Escherichia coli, Staphylococcus aureus, Bacillus cereus, Bacillus megaterium, Salmonella typhimurium, Shigella flexneri, and Vibrio cholerae are sensitive to ozonated water under various conditions (5, 6, 13, 17). Limited information on ozone's effectiveness against bacterial endospores (5, 17) and viruses (6, 20) as well as against eukaryotic pathogens including Cryptosporidium parvum (11, 21, 24) and Giardia lamblia and Giardia muris (12, 31, 32) also exists. The use of ozone in the food industry has been investigated for food preservation, shelf life extension, equipment sterilization, and improvement of food plant effluents (9, 14, 19, 2...

show abstract

“…Ozone, discovered by Schönbein in 1839, can be used instead of chlorine as an effective water disinfectant and is able to inactivate as many as 63 different human pathogenic bacteria (Bocci 2002), although some protozoa, oocysts, fungi and spores require a longer time of contact with ozone than bacteria and viruses (Ingram and Barnes 1954). It has been postulated that ozone may have a relevant therapeutic role in various types of infections because it generates reactive oxygen species (ROS) such as anion superoxide, hydroxyl radicals and hydrogen peroxide (H 2 O 2 ), notably produced by granulocytes and macrophages during an infectious process (Badwey and Karnowsky 1980;Babior 2000;Margalit et al 2001).…”

Section: Introductionmentioning

confidence: 99%

How much ozone bactericidal activity is compromised by plasma components?

Burgassi

Zanardi

Travagli

et al. 2009

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: Evaluation of bactericidal effect of different concentrations of ozone\ud when used (a) as a gas, or (b) dissolved in saline. The addition of hydrogen\ud peroxide or 4-hydroxynonenal dissolved in saline was also tested, as well as the\ud effect of human plasma.\ud Methods and Results: Staphylococcus aureus, methicillin-resistant Staph. aureus\ud (MRSA), and Pseudomonas aeruginosa, suspended in their culture media were\ud tested. While all bacteria suspended in protein-free saline were killed at high\ud ozone concentrations, they survived when as little as 5% human plasma was\ud present. Hydrogen peroxide was 100-fold less active than ozone and needed to\ud remain in contact with bacteria for at least 60 min. 4-hydroxynonenal\ud (2 lmol l)1) was inhibitory for proliferation of both Staph. aureus and MRSA,\ud but not for Ps. aeruginosa.\ud Conclusions: Ozone and the cascade of its derivative products are potent bactericidal\ud agents, but even small amounts of human plasma, hence of hydro- and\ud liposoluble antioxidants, in bacterial suspensions inhibit oxidation and protect\ud bacteria.\ud Significance and Impact of the Study: Any substantial in vivo cytocidal effect\ud of ozone and its derivatives can be excluded. On the other hand, topical and\ud continuous action of various ozone preparations remains valuable in a variety\ud of skin and mucosal infections

show abstract

Sterilization by Means of Ozone

Cited by 14 publications

References 18 publications

Sensorial and microbial effects of gaseous ozone on fresh scad (Trachurus trachurus)

Sensorial and microbial effects of gaseous ozone on fresh scad (Trachurus trachurus)

Efficacy of ozonated water against various food-related microorganisms

How much ozone bactericidal activity is compromised by plasma components?

Contact Info

Product

Resources

About