cycle. Every delivery of steam to the sterilizer can have different characteristics that change in every cycle. To ensure the air and NCG removal process and to control cost, we strongly recommend the use of a type-2 chemical indicator in every cycle (Table 1) by a reusable test device according to the European standard for nonbiological systems for use in sterilizers part 5, specification for indicator systems and process challenge devices for use in performance testing for small sterilizers type B and type S (EN 867-5). This process can confirm that the sterilizer chamber and packages are free from air and NCGs to assure optimum sterility according to the European standard requirements for medical devices to be designated sterile (EN 556). 8 Acknowledgments. None. Financial support. No financial support was provided relevant to this article.