Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide

Checinska, Aleksandra; Fruth, Ingrid A.; Green, Tonia L.; Crawford, Ronald L.; Paszczyński, Andrzej

doi:10.1016/j.mimet.2011.07.008

Cited by 51 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We observed that SAFR-032 is one of the most efficient spore formers we have used in our laboratory and it is able to form a very rigid biofilm that floats on the surface of liquid medium (14). There are still some questions to be answered.…”

Section: Discussionmentioning

confidence: 97%

Protection of Bacillus pumilus Spores by Catalases

Checinska

Burbank

Paszczyński

2012

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

ABSTRACTBacillus pumilusSAFR-032, isolated at spacecraft assembly facilities of the National Aeronautics and Space Administration Jet Propulsion Laboratory, is difficult to kill by the sterilization method of choice, which uses liquid or vapor hydrogen peroxide. We identified two manganese catalases, YjqC and BPUM_1305, in spore protein extracts of severalB. pumilusstrains by using PAGE and mass spectrometric analyses. While the BPUM_1305 catalase was present in six of theB. pumilusstrains tested, YjqC was not detected in ATCC 7061 and BG-B79. Furthermore, both catalases were localized in the spore coat layer along with laccase and superoxide dismutase. Although the initial catalase activity in ATCC 7061 spores was higher, it was less stable over time than the SAFR-032 enzyme. We propose that synergistic activity of YjqC and BPUM_1305, along with other coat oxidoreductases, contributes to the enhanced resistance ofB. pumilusspores to hydrogen peroxide. We observed that the product of the catalase reaction, gaseous oxygen, forms expanding vesicles on the spore surface, affecting the mechanical integrity of the coat layer, resulting in aggregation of the spores. The accumulation of oxygen gas and aggregations may play a crucial role in limiting further exposure ofBacillispore surfaces to hydrogen peroxide or other toxic chemicals when water is present.

show abstract

Section: Discussionmentioning

confidence: 97%

Protection of Bacillus pumilus Spores by Catalases

Checinska

Burbank

Paszczyński

2012

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Also, a high inoculum load of spores used in this study could have hindered the sporicidal effect of the treatments as spores are more susceptible to aggregation. Previous studies have suggested that inner spores deposited in a spore aggregate are protected by killed spores on the top, forming passive or active barriers which the SCCO 2 must diffuse through (Checinska, Fruth, Green, Crawford, & Paszczynski, 2011;Checinska, Burbank, & Paszczynski, 2012;Enomoto, Nakamura, Hakoda, & Amaya, 1997) to be effective. Nonetheless, the relatively high initial load of microorganisms used in the present study was deemed necessary to enable a comparison of the effects of the different hurdles applied, so that additive or synergistic treatment effects could be evaluated and for the assessment of the efficacy and potential of the different techniques used.…”

Section: Treatmentsmentioning

confidence: 99%

Synergistic effect of supercritical carbon dioxide and peracetic acid on microbial inactivation in shredded Mozzarella-type cheese and its storage stability at ambient temperature

2016

View full text Add to dashboard Cite

“…Microfluidic cell culture poses elicitation of specific sterilization requirements. Current methods of sterilization for the removal of microorganisms from medical devices include steam autoclaving, ethylene oxide treatment, UV and hydrogen peroxide treatment [7][8][9]. However, the techniques have obvious drawbacks and cannot be applied for sterilization of some materials such as polymeric medical devices and heat-sensitive biomaterials [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Sterilization with supercritical carbon dioxide (SC-CO 2 ) is reported as an alternative technique for sterilization of biological pathogens [7]. Carbon dioxide has many features such as low critical temperature and pressure, is inexpensive, non-toxic, inert and non-flammable.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sterilization of PMMA microfluidic chips by various techniques and investigation of material characteristics

Yavuz

Oliaei

Çetin

et al. 2016

The Journal of Supercritical Fluids

View full text Add to dashboard Cite

a b s t r a c tThe sterilization of microfluidic chips is a vital step of the fabrication process prior to the customer use in biomedical applications. The aim of this study was to analyze the influence of different sterilization techniques and to compare the characteristics of the material before and after sterilization of polymethylmethacrylate (PMMA) microchips. For this, supercritical carbon dioxide (SC-CO 2 ) along with standard sterilization methods such as ultraviolet (UV), heat (autoclaving), ethylene oxide (EtO) and hydrogen peroxide (H 2 O 2 ) were applied. The treated microchips were analyzed by Scanning Electron Microscopy, Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy and Laser Scanning Microscopy in order to ascertain any changes in the chemical structure and surface morphology. The optimum sterilization parameters for SC-CO 2 were elicited as 120 bar, 40 • C and 60 min which provided complete sterility and did not alter the main properties of the polymer along with EtO and H 2 O 2 sterilizations unlike heat and UV treatments. However, surface roughness and microchannel profiles were negatively affected. Although complete sterility was achieved, each protocol has its own strengths and weaknesses.

show abstract

Sterilization of biological pathogens using supercritical fluid carbon dioxide containing water and hydrogen peroxide

Cited by 51 publications

References 36 publications

Protection of Bacillus pumilus Spores by Catalases

Protection of Bacillus pumilus Spores by Catalases

Synergistic effect of supercritical carbon dioxide and peracetic acid on microbial inactivation in shredded Mozzarella-type cheese and its storage stability at ambient temperature

Sterilization of PMMA microfluidic chips by various techniques and investigation of material characteristics

Contact Info

Product

Resources

About