Sterilization of Chitosan Membranes for Use as Biomaterial

Nascimento, Imarally V.S.R.; Leal, Rita de Cássia A.; Fook, Marcus Vinícius Lia; Meira, Roberta Costa

doi:10.4028/www.scientific.net/msf.805.35

MSF

2014

DOI: 10.4028/www.scientific.net/msf.805.35

|View full text |Cite

Sterilization of Chitosan Membranes for Use as Biomaterial

Imarally V.S.R. Nascimento

Rita de Cássia A. Leal

Marcus Vinícius Lia Fook

et al.

Abstract: Sterilization is a procedure which promotes complete destruction or elimination of all forms of microorganisms. It is a fundamental stage in the processing of biomaterials, and functionality of any sterilization system must be determined by its effectiveness in exterminating microorganisms without harming or adversely affect the properties of medical devices. Some sterilization techniques can react with functional groups of the polymer leading to changes in the properties, affecting the biomaterial. This study… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2017

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Methods for atmospheric pressure plasma modification of chitosan surface to improve biocompatibility

Kamalov,

Endiiarova,

Osipov

et al. 2024

Polymer Engineering & Sci

View full text Add to dashboard Cite

This paper presents the optimal mode of plasma treatment of chitosan films to increase the proliferative activity of dermal fibroblasts and osteoblasts. For plasma modification of films, a dielectric barrier discharge in a flat electrode system and a plasma jet configuration at atmospheric pressure were used. The structural and chemical changes of chitosan were investigated using atomic force microscopy, infrared spectroscopy, and contact angle measurement. The optimal time for plasma treatment was determined using an in vitro test, which was found to be 1 min. The data of the MTT test performed on the 4th day of osteoblast cultivation show that when a chitosan sample was treated in plasma jet for 1 min, the proliferative activity of grown osteoblasts increases by 25%. However, the level of proliferative activity of human dermal fibroblasts was 5% higher on the film treated with the plasma jet. Modification by the plasma jet provided a more uniform microrelief, created an optimal contact angle of the polar liquid of 52° and led to an increase in the number of oxygen‐ and nitrogen‐containing groups on the surface.Highlights Modification of chitosan with DBD and PJ increases cell proliferation. The optimal contact angle of chitosan is 52° for proliferations of cells. Treatment of chitosan films with plasma jet is softer than in DBD.

show abstract

Methods for atmospheric pressure plasma modification of chitosan surface to improve biocompatibility

Kamalov,

Endiiarova,

Osipov

et al. 2024

Polymer Engineering & Sci

View full text Add to dashboard Cite

show abstract

Fabrication and characterization of hydrothermal cross-linked chitosan porous scaffolds for cartilage tissue engineering applications

Shamekhi

Rabiee

Mirzadeh

et al. 2017

Materials Science and Engineering: C

View full text Add to dashboard Cite

The use of various chemical cross-linking agents for the improvement of scaffolds physical and mechanical properties is a common practical method, which is limited by cytotoxicity effects. Due to exerting contract type forces, chondrocytes are known to implement shrinkage on the tissue engineered constructs, which can be avoided by the scaffold cross-linking. In the this research, chitosan scaffolds are cross-linked with hydrothermal treatment with autoclave sterilization time of 0, 10, 20 and 30min, to avoid the application of the traditional chemical toxic materials. The optimization studies with gel content and crosslink density measurements indicate that for 20min sterilization time, the gel content approaches to ~80%. The scaffolds are fully characterized by the conventional techniques such as SEM, porosity and permeability, XRD, compression, thermal analysis and dynamic mechanical thermal analysis (DMTA). FT-IR studies shows that autoclave inter-chain cross-linking reduces the amine group absorption at 1560cm and increase the absorption of N-acetylated groups at 1629cm. It is anticipated, that this observation evidenced by chitosan scaffold browning upon autoclave cross-linking is an indication of the familiar maillard reaction between amine moieties and carbonyl groups. The biodegradation rate analysis shows that chitosan scaffolds with lower concentrations, possess suitable degradation rate for cartilage tissue engineering applications. In addition, cytotoxicity analysis shows that fabricated scaffolds are biocompatible. The human articular chondrocytes seeding into 3D cross-linked scaffolds shows a higher viability and proliferation in comparison with the uncross-linked samples and 2D controls. Investigation of cell morphology on the scaffolds by SEM, shows a more spherical morphology of chondrocytes on the cross-linked scaffolds for 21days of in vitro culture.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Sterilization of Chitosan Membranes for Use as Biomaterial

Cited by 2 publications

References 20 publications

Methods for atmospheric pressure plasma modification of chitosan surface to improve biocompatibility

Methods for atmospheric pressure plasma modification of chitosan surface to improve biocompatibility

Fabrication and characterization of hydrothermal cross-linked chitosan porous scaffolds for cartilage tissue engineering applications

Contact Info

Product

Resources

About