Sterilization of collagen scaffolds designed for peripheral nerve regeneration: Effect on microstructure, degradation and cellular colonization

Monaco, Graziana; Cholas, Rahmatullah H.; Salvatore, Luca; Madaghiele, Marta; Sannino, Alessandro

doi:10.1016/j.msec.2016.10.030

Cited by 45 publications

(42 citation statements)

References 49 publications

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“…In stem cell therapies based on 3D scaffolds, an artificial ECM is constructed and designed to degrade so that it will be replaced as the new tissue forms. Generally, natural materials degrade through enzymatic activity and synthetic organic materials degrade hydrolytically, while porous inorganic materials are much harder to degrade . For the desired scaffold function in clinical therapies, the degradation mechanism based on the physiological conditions of the implantation site needs to be carefully considered.…”

Section: D Culture For Clinical Applicationsmentioning

confidence: 99%

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

et al. 2018

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Stem-cell-based therapies have the potential to provide novel solutions for the treatment of a variety of diseases, but the main obstacles to such therapies lie in the uncontrolled differentiation and functional engraftment of implanted tissues. The physicochemical microenvironment controls the self-renewal and differentiation of stem cells, and the key step in mimicking the stem cell microenvironment is to construct a more physiologically relevant 3D culture system. Material-based 3D assemblies of stem cells facilitate the cellular interactions that promote morphogenesis and tissue organization in a similar manner to that which occurs during embryogenesis. Both natural and artificial materials can be used to create 3D scaffolds, and synthetic organic and inorganic porous materials are the two main kinds of artificial materials. Nanotechnology provides new opportunities to design novel advanced materials with special physicochemical properties for 3D stem cell culture and transplantation. Herein, the advances and advantages of 3D scaffold materials, especially with respect to stem-cell-based therapies, are first outlined. Second, the stem cell biology in 3D scaffold materials is reviewed. Third, the progress and basic principles of developing 3D scaffold materials for clinical applications in tissue engineering and regenerative medicine are reviewed.

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Section: D Culture For Clinical Applicationsmentioning

confidence: 99%

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

et al. 2018

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“…Also, collagen conduit application on major peripheral nerve injuries is limited to median and ulnar nerve repairs at the wrist and only observed as an alternative to the classic epineural suture repair [78]. In a recent study, Monaco and colleagues [118] investigated the effect of three different sterilization methods, dry heat, ethylene oxide and electron beam radiation, on the properties of cylindrical collagen scaffolds with longitudinally oriented pore channels, specifically designed for peripheral nerve regeneration. Ethylene oxide exposure demonstrated to be the most suitable method for the sterilization of the proposed scaffolds, since β-sterilization significantly augmented scaffold enzymatic degradation.…”

Section: Collagenmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…The material sterilization process is intended to remove or destroy all forms of life, macroscopic or microscopic, from the product of interest, ensuring that the inactivation of cellular enzymes and toxins occurs. According to the sterility assurance requirements described in pharmacopoeias, a sterility assurance level (SAL) of 10 -6 (statistical probability of finding one contaminated unit is equal to 1:1000000) is accepted for sterilization procedures of materials and pharmaceutical products [55,[250][251][252].…”

Section: Sterilization Of the Polymer Materialsmentioning

confidence: 99%

Drug Micro-Carriers Based on Polymers and Their Sterilization

Costa¹,

Pereira²,

Silva³

et al. 2018

ChChT

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Sterilization of collagen scaffolds designed for peripheral nerve regeneration: Effect on microstructure, degradation and cellular colonization

Cited by 45 publications

References 49 publications

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

Looking into the Future: Toward Advanced 3D Biomaterials for Stem‐Cell‐Based Regenerative Medicine

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Drug Micro-Carriers Based on Polymers and Their Sterilization

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