Sterilization techniques for biodegradable scaffolds in tissue engineering applications

Dai, Zheng; Ronholm, Jennifer; Tian, Yaping; Sethi, Benu; Cao, Xudong

doi:10.1177/2041731416648810

Cited by 263 publications

(268 citation statements)

References 86 publications

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“…Diverse sterilization techniques have been related to inducing changes in the characteristics of materials and properties, depending on the physicochemical nature of the polymers [35,36]. Thus, the intensive chemical evaluation after sterilization method is necessary as the doses and times of sterilization application vary according to the material.…”

Section: Fourier-transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

et al. 2020

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The sterilization processes of nanoparticles (NP) by autoclaving and filtration are two of the most utilized methods in the pharmaceutical industry but are not always a viable option. For this reason, the search for alternative options such as UV and gamma radiation is of interest. In this work, we evaluated both types of sterilization on two types of NP in solid state widely employed in the literature for biomedical applications, poly-(ε-caprolactone) and poly(d,l-lactide-co-glycolide) acid NP stabilized with polyvinyl alcohol. Physicochemical properties and cell viability were studied pre-and post-sterilization. The efficiency of irradiation sterilization was performed by a test of sterility using 1 × 10 8 CFU/mL of Escherichia coli, Staphylococcus aureus, and Candida albicans. Microbiological monitoring revealed that both methods were sufficient for sterilization. After the UV irradiation sterilization (100 µJ/cm 2 ), no substantial changes were observed in the physicochemical properties of the NP or in the interaction or morphology of human glial cells, though 5 and 10 kGy of gamma irradiation showed slight changes of NP size as well as a decrease in cell viability (from 100 µg/mL of NP). At 5 kGy of radiation doses, the presence of trehalose as cryoprotectant reduces the cell damage with high concentrations of NP, but this did not occur at 10 kGy. Therefore, these methods could be highly effective and low-processing-time options for sterilizing NP for medical purposes. However, we suggest validating each NP system because these generally are of different polymer-composition systems.

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Section: Fourier-transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

et al. 2020

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“…For cytocompatibility testing, constructs were sterilized using peracetic acid (PAA; Sigma‐Aldrich, St. Louis, MO) as described previously; scaffolds were immersed with agitation in 0.1% PAA in PBS for 2 h, rinsed in sterile PBS, and then incubated overnight in Dulbecco's Modified Eagle Medium (DMEM: Fisher Scientific, Waltham, MA) containing 50% fetal bovine serum (FBS: Atlanta Biologicals, Flowery Branch, GA) and 1% antibiotic/−mycotic (AB/AM: Fisher Scientific). Sterile scaffolds (Ø6 mm × 2–4 mm height; n = 4/group for ABNP, MES Only, EtOH/MES, Comp+EtOH/MES) were each seeded with 1.0 × 10 6 (passage 6) human Adipose Derive Stem Cells (hADSCs: Fisher Scientific) in 150 μL of media.…”

Section: Methodsmentioning

confidence: 99%

Ethanol‐mediated compaction and cross‐linking enhance mechanical properties and degradation resistance while maintaining cytocompatibility of a nucleus pulposus scaffold

2019

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Intervertebral disc degeneration is a complex, cell‐mediated process originating in the nucleus pulposus (NP) and is associated with extracellular matrix catabolism leading to disc height loss and impaired spine kinematics. Previously, we developed an acellular bovine NP (ABNP) for NP replacement that emulated human NP matrix composition and supported cell seeding; however, its mechanical properties were lower than those reported for human NP. To address this, we investigated ethanol‐mediated compaction and cross‐linking to enhance the ABNP's dynamic mechanical properties and degradation resistance while maintaining its cytocompatibility. First, volumetric and mechanical effects of compaction only were confirmed by evaluating scaffolds after various immersion times in buffered 28% ethanol. It was found that compaction reached equilibrium at ~30% compaction after 45 min, and dynamic mechanical properties significantly increased 2–6× after 120 min of submersion. This was incorporated into a cross‐linking treatment, through which scaffolds were subjected to 120 min precompaction in buffered 28% ethanol prior to carbodiimide cross‐linking. Their dynamic mechanical properties were evaluated before and after accelerated degradation by ADAMTS‐5 or MMP‐13. Cytocompatibility was determined by seeding stem cells onto scaffolds and evaluating viability through metabolic activity and fluorescent staining. Compacted and cross‐linked scaffolds showed significant increases in DMA properties without detrimentally altering their cytocompatibility, and these mechanical gains were maintained following enzymatic exposure. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2488–2499, 2019.

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“…It was proved that some spores of bacteria are resistant to the irradiation due to a low concentration of water in their protoplasm. 5 resistant to the irradiation in comparison with aliphatic ones. and Rubrobacter spp., which are characterized by highly proficient DNA repair system and oxidative stress alleviation in their cells.…”

Section: Introductionmentioning

confidence: 94%

“…3 The most promising and widely described in the literature are ionizing techniques such as gamma and electron beam irradiation. 5 The highest resistance and survive up to doses greater than 25 kGy of γ-ray showed Deinococcus spp. 4 According to the literature, e-beam irradiation has the ability to inactivate gram-negative and gram-positive bacteria, molds, yeasts, most viruses, and some bacterial spores.…”

Section: Introductionmentioning

confidence: 99%

The effects of electron beam radiation on material properties and degradation of commercial PBAT/PLA blend

Iuliano

Nowacka

Rybak

et al. 2019

J of Applied Polymer Sci

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This work investigates the potential application of e-beam radiation for sterilization of food packaging made of commercial polyester blend of poly(butylene adipate-co-terephthalate) and polylactide known under the trade name Ecovio 23B1. Ecovio film was irradiated at doses of 5, 13, and 26 kGy and the effect of sterilization on the microorganism inactivation ability was performed. The influence of radiation doses on structural and packaging properties such as tensile strength, barrier, and migration properties was also studied. 1 H NMR and FTIR analysis revealed no significant changes in the polymer structure, however, the molar mass of irradiated samples decreased with increasing radiation dose. This result indicated the occurrence of degradation process during radiation, which was confirmed by the thermal analysis and accelerated enzymatic degradation. The packaging properties did not changed significantly and the overall migration for 10% ethanol, isooctane, and MPPO was below the limit for all of the studied samples.

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Sterilization techniques for biodegradable scaffolds in tissue engineering applications

Cited by 263 publications

References 86 publications

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

Effect of UV and Gamma Irradiation Sterilization Processes in the Properties of Different Polymeric Nanoparticles for Biomedical Applications

Ethanol‐mediated compaction and cross‐linking enhance mechanical properties and degradation resistance while maintaining cytocompatibility of a nucleus pulposus scaffold

The effects of electron beam radiation on material properties and degradation of commercial PBAT/PLA blend

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