Steroid alkaloids of the plant and animal Kingdoms

Kul'kova, V. V.; Shakirov, R.; D'yakonov, A. L.

doi:10.1007/bf02234919

Cited by 12 publications

(4 citation statements)

References 54 publications

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“…The biosynthesis pathway of these steroidal alkaloids is still largely unknown. Steroid are synthesized via two pathways in plants: the classical mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway that leads to the synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) (Kul'Kova et al 1999). These intermediates serve as the substrates that undergo a cascade of chemical conversions along with the formation of metabolite intermediates, such as geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), squalene, cycloartenol, through terpenoid backbone biosynthesis (Schaller 2010).…”

Section: Expression Of the Major Steroidal Alkaloid Biosynthesis-relamentioning

confidence: 99%

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Zhao

Zhang

et al. 2018

3 Biotech

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The bulbs of is wildly used in traditional Chinese medicine to treat lung-related disease, which has recently been found to have antitussive, anti-inflammatory, antihypertensive and anti-tumor activity. Steroidal alkaloids are the major effective ingredients of. In the current study, we demonstrated an efficient strategy for bulb regeneration in vitro by cytokinin/auxin induction. Our data showed that the regenerated bulbs accumulated higher alkaloid content that the wild ones. We further performed RNA-seq and bioinformatics analysis to study the gene expression profile, especially those related to alkaloids biosynthesis. KEGG pathway annotation identified genes related to "Metabolic pathways" were the most abundant (2644, 26.0%), followed by those for "Biosynthesis of secondary metabolites" (1319, 13.0%) among the 113,865 unigenes identified. Further analysis suggested MEP pathway, other than MVA pathway, might be the major route for steroidal alkaloid biosynthesis of, as all the key genes in this pathway were found to be unregulated in our study. We also showed that accumulation of different phytochemicals was linked to plant hormone addition. Our current study demonstrated that in vitro cultivation is a promising strategy for mass production of steroidal alkaloids for pharmacological industry.

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Section: Expression Of the Major Steroidal Alkaloid Biosynthesis-relamentioning

confidence: 99%

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Zhao

Zhang

et al. 2018

3 Biotech

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“…2 Comparative analysis of significantly enriched GO and KEGG pathways. A Go Term (BP) (A alkaloid biosynthetic process; B specialized metabolite biosynthetic process; C. Steroid metabolic process; D. steroid biosynthetic process; E. C21-steroid hormone metabolic process; F. sterol biosynthetic process; G. seed development; H. reproductive system development; I. photosynthesis; J. isoprenoid metabolic process; GS-blue; JY-pink; SS-green; TB-yellow; WB-gray); B KEGG enrichment analysis [21,22]; C 10 enriched KEGG pathways enriched in BFC compared with BFT [23] acid (MVA) signaling pathways are essential routes involved in the biosynthesis of dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP), which are precursors of steroidal alkaloids [24]. We found that Cluster-1034.25709 (predicted to be acetyl-CoA C-acetyltransferase, AACT), Cluster-1034.19505 (predicted to be 3-hydroxy-3-methylglutaryl-CoA synthase, HMGS), Cluster-1034.25580 (predicted to be 3-hydroxy-3-methylglutaryl-CoA reductase, HMGR), Cluster-1034.9280 (predicted to be mevalonate kinase, MK), Cluster-1034.31238 (predicted to be 5-phosphomevalonate kinase, PMK), and Cluster-1034.34747(predicted to be mevalonate 5-diphosphate decarboxylase, MVD), which have been reported to participate in the DMAPP/IPP synthesis through the MVA pathway in other plants [25], were expressed higher in BFC than that in BFT (Fig.…”

Section: Analysis and Identification Of Candidate Genes Possibly Invo...mentioning

confidence: 99%

Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses

Liao

et al. 2022

BMC Genomics

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Background Bulbus Fritillariae Cirrhosae (BFC) is an endangered high-altitude medicine and food homology plant with anti-tumor, anti-asthmatic, and antitussive activities as it contains a variety of active ingredients, especially steroidal alkaloids. Bulbus Fritillariae Thunbergia (BFT) is another species of Fritillaria that grows at lower altitude areas. Production of plant-derived active ingredients through a synthetic biology strategy is one of the current hot topics in biological research, which requires a complete understanding of the related molecular pathways. Our knowledge of the steroidal alkaloid biosynthesis in Fritillaria species is still very limited. Results To promote our understanding of these pathways, we performed non-target metabolomics and transcriptome analysis of BFC and BFT. Metabolomics analysis identified 1288 metabolites in BFC and BFT in total. Steroidal alkaloids, including the proposed active ingredients of Fritillaria species peimine, peimisine, peiminine, etc., were the most abundant alkaloids detected. Our metabolomics data also showed that the contents of the majority of the steroidal alkaloids in BFC were higher than in BFT. Further, our comparative transcriptome analyses between BFC and BFT identified differentially expressed gene sets among these species, which are potentially involved in the alkaloids biosynthesis of BFC. Conclusion These findings promote our understanding of the mechanism of steroidal alkaloids biosynthesis in Fritillaria species.

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“…Jervine is one of the steroidal alkaloids isolated from Veratrum album 11 (Figure 1). It has anticancer and antitumor properties 12,13 . N-acetylcysteine (NAC) has been used clinically since the 1970s.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of jervine purified from Veratrum album on paracetamol-induced liver toxicity in rats

Yiğit,

Aydın,

Bitiktaş

et al. 2024

Cukurova Medical Journal

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Purpose: Since paracetamol toxicity is a very common type of poisoning, we planned to investigate whether Jervine has an effect on paracetamol toxicity by utilizing its anti-inflammatory effect. Materials and Methods: In our study, 42 Sprague Dawley rats of 8 weeks of age were used. Seven groups were formed with 6 animals in each group. At the 24th hour of the study, all groups underwent laparotomy under anesthesia, and liver dissection was performed. Hematoxylin and Eosin (H&E) staining was performed to evaluate liver histopathology. SOD, CAT, GSH, and MDA levels were analyzed biochemically. Results: Histopathological, while liver tissues were normal in the control group, we observed degeneration areas, inflammation, and hemorrhage in the paracetamol group. Jervine reduced the severity of paracetamol toxicity and prevented liver damage. Jervine significantly increased SOD levels. Paracetamol administration significantly decreased CAT levels. Paracetamol significantly decreased GSH levels compared to the control group. Conclusion: Jervine reduced the adverse effects of paracetamol toxicity on liver tissue, such as degeneration, inflammation, and hemorrhage. Jervine increased antioxidant activity and reduced the harmful effects of NAPQI, the toxic metabolite of paracetamol, on liver tissue.

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Steroid alkaloids of the plant and animal Kingdoms

Cited by 12 publications

References 54 publications

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis

Integrative analysis of the steroidal alkaloids distribution and biosynthesis of bulbs Fritillariae Cirrhosae through metabolome and transcriptome analyses

Protective effects of jervine purified from Veratrum album on paracetamol-induced liver toxicity in rats

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