Steroid-depleted polycystic ovarian syndrome serum promotes<i>in vitro</i>oocyte maturation and embryo development

Eyvaznejad, Elham; Nouri, Mohammad; Ghasemzadeh, Aliyeh; Mehdizadeh, Amir; Shahnazi, Vahideh; Asghari, Samira; Mardomi, Alireza; Darabi, Masoud

doi:10.1080/09513590.2018.1431770

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…p < .01. SCD1, stearoyl-CoA desaturase-1 media with lipid-depleted serum obtained from normal women and patients with PCOS considerably improved the capacity of IVM system in oocyte maturation (Eyvaznejad et al, 2018;Mardomi et al, 2019).…”

Section: Discussionmentioning

confidence: 98%

“…However, it has already been stated that cumulus cells are capable to synthesize and secrete E2 and P4 in vitro (Barrera et al, 2018; Beker, Colenbrander, & Bevers, 2002; Blaschka et al, 2019). Based on two previous studies, supplemented culture media with lipid‐depleted serum obtained from normal women and patients with PCOS considerably improved the capacity of IVM system in oocyte maturation (Eyvaznejad et al, 2018; Mardomi et al, 2019). Hence, we applied charcoal‐stripped endometriosis serum to enhance the efficacy of IVM system (Cao et al, 2009; Castro, Morrill, & Pope, 2000).…”

Section: Discussionmentioning

confidence: 99%

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Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems

Asghari

Nouri

Darabi

et al. 2020

Journal Cellular Physiology

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In vitro maturation (IVM) is a novel approach to overcome the adverse effects of human in vitro fertilization (IVF). The aim of the present study is to evaluate the effect of total and steroid-depleted serum obtained from patients with endometriosis on IVM outcome as supplementation for this system. To this purpose, patients with endometriosis were selected according to in/excluding criteria. Germinal vesicles (GVs) and cumulus cells were treated with 10% of each serum. The expression levels of stearoyl CoA desaturase 1 (SCD 1) and cyclooxygenase-2 (COX-2) genes were evaluated by RT-qPCR. Gas-liquid chromatography and flow cytometry were performed to analyze fatty acids composition and apoptosis. The mRNA expression levels of SCD1 (2.47 fold) and COX-2 (6.4 fold), and also the synthesis of oleate, linoleate, and arachidonate were increased (1.19, 1.06, and 2.37 folds, respectively) in cumulus cells treated with steroid-depleted serum (p < .05). The synthesis of palmitate, palmitoleate, and stearate (0.995, 0.67, and 0.7 folds, respectively) and also the rate of apoptosis were significantly decreased in these cells (p < .05). Moreover, GVs cultured in steroid-depleted group showed a significantly higher rate of maturation (p < .001). Overall, our findings imply a new insight into the expansion of IVM system in oocytes development.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems

Asghari

Nouri

Darabi

et al. 2020

Journal Cellular Physiology

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Dynamic role of Scd1 gene during mouse oocyte growth and maturation

Niu

Wang

et al. 2023

International Journal of Biological Macromolecules

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Human charcoal-stripped serum supplementation enhances both the stearoyl-coenzyme a desaturase 1 activity of cumulus cells and the in vitro maturation of oocytes

Mardomi

Nouri

Farzadi³

et al. 2018

Human Fertility

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Obtaining a better outcome in assisted reproductive technology remains to be attained. In the case of in vitro fertilization (IVF), oocyte maturity is paramount for achieving a successful pregnancy. Maternal serum supplementation of in vitro maturation (IVM) medium can increase the rate of oocyte IVM. The aim of the present study was to compare the effect of whole and charcoal-stripped serum supplementation on IVM and the activity index of stearoyl-coenzyme A desaturase 1 (SCD1) in cumulus cells enclosing the oocyte as a molecular indicator of oocyte quality. Cumulus cells and germinal vesicle immature oocytes were collected from 76 women with polycystic ovarian syndrome during an IVF cycle. Serum samples were pooled from healthy women and were applied as whole or charcoal-stripped serum supplements. SCD1 expression and activity were measured by quantitative polymerase chain reaction (PCR) and gas-liquid chromatography, respectively. Charcoal-stripped serum at an amount of 10% showed a higher potency in increasing the SCD1 expression and activity index than whole serum (>1.5 fold, p < 0.001). An increase in the IVM rate was also observed in oocytes cultured in the presence of 10% charcoal-stripped serum compared to the control group (1.9 fold, p = 0.031). Therefore, charcoal-based lipid depletion as a simple and preparative strategy may increase the beneficial effect of serum supplementation in oocyte IVM culture.

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Steroid-depleted polycystic ovarian syndrome serum promotesin vitrooocyte maturation and embryo development

Cited by 3 publications

References 35 publications

Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems

Steroid‐depleted endometriosis serum improves oocyte maturation in IVM systems

Dynamic role of Scd1 gene during mouse oocyte growth and maturation

Human charcoal-stripped serum supplementation enhances both the stearoyl-coenzyme a desaturase 1 activity of cumulus cells and the in vitro maturation of oocytes

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