Steroid-eluting contact lenses for corneal and intraocular inflammation

Bengani, Lokendrakumar C.; Kobashi, Hidenaga; Ross, Amy E.; Zhai, Hualei; Salvador-Culla, Borja; Tulsan, Rekha; Kolovou, Paraskevi E.; Mittal, Sanjay; Chauhan, Sunil; Kohane, Daniel S.; Ciolino, Joseph B.

doi:10.1016/j.actbio.2020.08.013

Cited by 48 publications

(28 citation statements)

References 74 publications

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“…Consistent with other research results, compared with eye drop, SCLs significantly prolonged retention time of PFD in vitro and in vivo (Maulvi et al, 2018;Gote et al, 2019;Bengani et al, 2020;Zhang et al, 2020). Thicker SCLs slowed down drug release in vitro, because molecules of drug within Cell viability of HCE-T cells were above 90% and had no significant change at all measured time points compared with control group; (B) the measured thickness of corneas of rabbits before and after safety test, the data was shown as mean ± SDs (n ¼ 6, ÃÃÃ P < .001); (C) picture of measured thickness of corneas of rabbits before the test (祄¼lm); (D) picture of measured thickness of corneas of rabbits after the test (祄¼lm); (E) histological picture of corneas of rabbits after wearing SCLs for consecutive seven days; (F) picture of histology of irises of rabbits after wearing SCLs for consecutive seven days.…”

Section: Discussionsupporting

confidence: 90%

“…Also, there are various innovated SCLs for ocular drug delivery in recent years to improve bioavailability. Researchers have made important progress in prolonging functional time of drugs in vitro an in vivo (Maulvi et al, 2018;Gote et al, 2019;Bengani et al, 2020;Zhang et al, 2020). In this study, we increased drug loading capacity in PVA film embedded SCLs, and evaluated drug release in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Controllable release of pirfenidone by polyvinyl alcohol film embedded soft contact lenses in vitro and in vivo

Chong

et al. 2021

Drug Delivery

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To increase the amount of pirfenidone (PFD) loaded in polyvinyl alcohol (PVA) film embedded soft contact lens (SCL), and evaluate its function of sustaining delivery of drug in vitro and in vivo . Drug loading efficiency within PVA film and SCLs, drug release from SCLs in vitro , and the effects of parameters of SCLs and external environment on drug release in vitro were evaluated by ultraviolet–visible spectrophotometer at 312 nm. Safety of SCLs was evaluated in vitro by transformed human corneal epithelial cell. Safety in vivo was determined by optical coherence tomography and histology of anterior segment of rabbits. Drug release study in tear fluid and aqueous humor were measured by ultra-performance liquid chromatography. SCLs had smooth surface and were fit for experimental rabbits. Amount of PFD in PVA film and SCLs were 153.515 μg ± 12.508 and 127.438 μg ± 19.674, respectively, PFD in PVA film was significantly higher than SCLs ( p =.006) and closed to 150 μg (targeting amount of PFD to be loaded). Thickness of SCLs, molecular weight of PVA, and amount of PVA used in SCLs affected drug release in vitro significantly. Thickness of PVA film and amount of drug in SCLs had no effect on drug release rate in vitro . SCLs were safe in vitro and in vivo , PFD released from SCLs could be detected around 12 hours in tears and aqueous humor, and the concentration of drug was higher than eye drop at all detected time points while amount of PFD in SCLs was lower than eye drop. Drug loaded PVA film embedded SCLs may be a promising ocular drug delivery system.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Controllable release of pirfenidone by polyvinyl alcohol film embedded soft contact lenses in vitro and in vivo

Chong

et al. 2021

Drug Delivery

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“…The scores involve weighting and summing six components of directly observable changes of the eye's anterior segment, including the density and area of corneal opacification, severity of iritis, conjunctival redness, edema, and discharge. [31][32][33][34][35][36]…”

Section: Ocular Toxicology: the Draize Eye Testmentioning

confidence: 99%

Baicalein Protects Against Aspergillus fumigatus Keratitis by Reducing Fungal Load and Inhibiting TSLP-Induced Inflammatory Response

Zhu

Peng

Zhang

et al. 2021

Invest. Ophthalmol. Vis. Sci.

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Purpose To investigate the antifungal and anti-inflammatory effects of baicalein on Aspergillus fumigatus ( A. fumigatus ) keratitis and the underlying mechanisms. Methods The noncytotoxic antifungal concentration of baicalein was determined using CCK8, cell scratch assay, minimum inhibitory concentration, biofilm formation, scanning electron microscopy, propidium iodide uptake test and adherence assay in vitro and Draize test in vivo. In fungal keratitis (FK) mouse models, clinical score and plate count were used to evaluate FK severity, and myeloperoxidase assay and immunofluorescence staining were performed to examine neutrophil infiltration and activity. Real-time PCR, ELISA, and Western blot were performed to explore the anti-inflammatory activity of baicalein and the underlying mechanisms in vivo and in vitro. Results Baicalein at 0.25 mM (noncytotoxic) significantly inhibited A. fumigatus growth, biofilm formation, and adhesion in vitro. In A. fumigatus keratitis mice, baicalein mitigated FK severity, reduced fungal load, and inhibited neutrophil infiltration and activity. Baicalein not only suppressed mRNA and protein levels of proinflammatory factors IL-1β, IL-6, and TNF-α, but also inhibited the expression of thymic stromal lymphopoietin (TSLP) and TSLP receptor (TSLPR) in vivo and in vitro. In HCECs, mRNA and protein levels of IL-1β, IL-6, and TNF-α were significantly lower in the TSLP siRNA–treated group, while higher in the rTSLP-treated group than in the corresponding control. Baicalein treatment significantly inhibited rTSLP induced the expression of IL-1β, IL-6, and TNF-α. Conclusions Baicalein plays a protective role in mouse A. fumigatus keratitis by inhibiting fungal growth, biofilm formation, and adhesion, and suppressing inflammatory response via downregulation of the TSLP/TSLPR pathway.

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“…However, the results have shown that the amount of active substances that are resorbed and released are too small to have any clinical significance and to create a controlled drug release, the contact lenses need to be modified for the purpose (Bengani et al. 2020; Zhang et al. 2020).…”

Section: Introductionmentioning

confidence: 99%

“…To increase the bioavailability of ocular drugs, many attempts have been made to load existing contact lens types by soaking them in ophthalmic solutions. However, the results have shown that the amount of active substances that are resorbed and released are too small to have any clinical significance and to create a controlled drug release, the contact lenses need to be modified for the purpose (Bengani et al 2020;Zhang et al 2020). Most studies on the effects of ophthalmic solutions on contact lenses are performed in vitro.…”

Section: Introductionmentioning

confidence: 99%

Rupture and chemical accumulation in contact lenses with dexamethasone eye drop administration after congenital cataract surgery

Lima

Erdal

Adolfsson

et al. 2021

Acta Ophthalmologica

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Purpose To investigate whether contact lenses used after surgery for congenital cataracts act as a depot for dexamethasone, which would allow the prescribed amount of drops to be reduced, and to examine whether the preservative benzalkonium chloride accumulates in the contact lens matrix, which would suggest a need for more frequent replacements. Methods Contact lenses (n = 10) worn by infants treated with dexamethasone eye drops after congenital cataract surgery were analysed with scanning electron microscopy, UV‐vis, 1H‐NMR and LDI‐MS for chemical deposits and for changes on the contact lens surface. Unused lenses (n = 5) and lenses (n = 4) from patients with no eye drop treatment were analysed as reference. Results The treated contact lenses displayed ruptured surfaces in comparison with unused and reference lenses. Dexamethasone and BAK were not detected in any of the lenses. A polyethylene oxide component was found in the treated lenses, likely originating from the dexamethasone eye drops or the contact lens solution. Conclusion Dexamethasone and BAK do not accumulate in the contact lenses, and a depot effect of any clinical significance is unlikely. Therefore, the number of drops given after surgery should remain the same regardless of whether the child has contact lenses. The ruptured surface may both decrease the child’s comfort and increase the risk of microbial adhesion, and so it is recommended that contact lenses should be replaced once a month throughout the course of anti‐inflammatory eye drop treatment after surgery for congenital cataract.

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Steroid-eluting contact lenses for corneal and intraocular inflammation

Cited by 48 publications

References 74 publications

Controllable release of pirfenidone by polyvinyl alcohol film embedded soft contact lenses in vitro and in vivo

Controllable release of pirfenidone by polyvinyl alcohol film embedded soft contact lenses in vitro and in vivo

Baicalein Protects Against Aspergillus fumigatus Keratitis by Reducing Fungal Load and Inhibiting TSLP-Induced Inflammatory Response

Rupture and chemical accumulation in contact lenses with dexamethasone eye drop administration after congenital cataract surgery

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