Steroid Hormone Regulation of Sexual Reproduction in Achlya

Expression and Inheritance of Sexual Preference and Selfing Potential inPhytophthora infestans

“…The rate of local oscillations in the concentrations of the sex hormones or their receptors may also influence the propensity of isolates to simultaneously form the male and female structures required for selfing to occur. The structure of the sex hormones of Phytophthora are unknown, but may be distinct from those of Achlya (Timberlake and Orr, 1984) since the Achlya substances do not induce sexual differentiation within single cultures of P. infestans or alter the sexual preference of strains in mating cultures (unpublished results). The structural determination of the Phytophthora sex hormones could be assisted by the GUS technique employed in this study, which could be used as a bioassay to assist in their purification.…”

Steroid hormone regulation of the Achlya ambisexualis 85-kilodalton heat shock protein, a component of the Achlya steroid receptor complex.

“…Taken together, these data suggest that similar or identical 85-kDa proteins are independently regulated by the steroid hormone antheridiol and by heat shock and that this protein is part of the Achlya steroid receptor complex. Our results demonstrate that the association of hsp9O family proteins with steroid receptors observed in mammals and birds extends also to the eucaryotic microbes and suggest that this association may have evolved early in steroid-responsive systems.The water mold Achlya ambisexualis is a eucaryotic, filamentous fungus in which sexual reproduction is regulated by well-characterized steroid hormones (4,22,54). There are at least two mating types in A. ambisexualis, and these are usually referred to as male and female.…”

“…The water mold Achlya ambisexualis is a eucaryotic, filamentous fungus in which sexual reproduction is regulated by well-characterized steroid hormones (4,22,54). There are at least two mating types in A. ambisexualis, and these are usually referred to as male and female.…”

Regulation of hsp90 and hsp70 Genes during Antheridiol-Induced Hyphal Branching in the OomyceteAchlya ambisexualis

“…When antheridiol is added to vegetative mycelia of A. ambisexualis E87, a strain that forms antheridia in crosses with most other A. ambisexualis strains, the hyphae undergo antheridial branching (reviewed in Barksdale, 1969;Timberlake and Orr, 1984). The synthesis of several proteins in the mycelium is increased during hormoneinduced branching Silver, 1986b, 1987;Brunt et al, 1990).…”