Steroid hormones (17β-estradiol and hydrocortisone) upregulate hepatocyte nuclear factor (HNF)-3β and insulin-like growth factors I and II expression in the gonads of tilapia (Oreochromis mossambicus) in vitro

Huang, Wei‐Tung; Yu, Haocheng; Hsu, Chih Chien; Liao, Ching-Fong; Gong, Hong‐Yi; Lin, Cliff Ji-Fan; Wu, Jen‐Leih; Weng, Ching‐Feng

doi:10.1016/j.theriogenology.2007.07.016

Cited by 8 publications

(27 citation statements)

References 52 publications

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“…Some of these processes have been shown to be influenced by IGF signaling (Wood et al 2005, Huang et al 2007, Schlueter et al 2007, Reinecke 2010. The growth promoting effects of cortisol on embryonic cell proliferation and embryo growth in this study (Table 2), together with the changes in the expression of IGF system gene expressions in early embryos (Figs 5 and 6), are consistent with the proposition that cortisol activates the IGF system signaling pathway in early embryonic cells.…”

Section: K1supporting

confidence: 88%

“…IGF system activation may then contribute to the promotion of early embryonic cell proliferation and embryonic growth. In post-embryonic fish, cortisol-activated stimulation of the expression of IGF-and GH system-related genes has been described in Mozambique tilapia (Oreochromis mossambicus) ovarian follicles (Huang et al 2007), hepatocytes (Pierce et al 2011), and rainbow trout lymphocytes (Yada et al 2005). In mammalian embryos, the role of GR activation in cellular maturation and growth, most notably stem cell differentiation, is well established (Derfoul et al 2006, Phillips et al 2006, Mikami et al 2008, Stewart et al 2008.…”

Section: K1mentioning

confidence: 99%

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Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Li¹,

Leatherland²,

Vijayan³

et al. 2012

Journal of Endocrinology

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Increased in ovo cortisol content of rainbow trout oocytes from w3 . 5 to w5 . 0 ng.oocyte K1 before fertilization enhances the growth of embryos and juveniles and changes the long-term expression pattern of IGF-related genes. This study used embryos reared from oocytes enriched with cortisol and the glucocorticoid receptor (GR) antagonist, RU486, to determine whether the growth-promoting actions of cortisol involve GR protein activation and modulation of gr expression. Whole-mount in situ immunohistofluorescence studies of zygotes showed that enhanced oocyte cortisol increased the immunofluorescent GR signal and activated the relocation of GR from a general distribution throughout the cytoplasm to an accumulation in the perinuclear cytoplasm. In ovo cortisol treatment increased the number of embryonic cells within 48-h post-fertilization, and RU486 partially suppressed this cortisol stimulation of cell duplication. In addition, there was complex interplay between the expression of gr and igf system-related genes spatiotemporally in the different treatment groups, suggesting a role for GR in the regulation of the expression of development. Taken together, these findings indicate an essential role for GR in the regulation of epigenomic events in very early embryos that promoted the long-term growth effects of the embryos and juvenile fish. Moreover, the pretreatment of the oocyte with RU486 had a significant suppressive effect on the maternal mRNA transcript number of gr and igf system-related genes in oocytes and very early stage embryos, suggesting an action of antagonist on the stability of the maternal transcriptome.

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Section: K1supporting

confidence: 88%

Section: K1mentioning

confidence: 99%

Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Li¹,

Leatherland²,

Vijayan³

et al. 2012

Journal of Endocrinology

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“…The FOXA proteins were first identified in liver nuclear extracts as transcription factors essential for the regulation of hepatocyte-specific expression of several target genes (30). Genes for Foxa1 and Foxa2 expressed in the mouse endometrium and uterine glands (10) and E 2 have been shown to upregulate the expression of Foxa2 mRNA at concentrations of 0.1-10 nM (20). Therefore, our data suggest that estrogen may regulate the action of Foxa family transcription factors also in bovine uterus, which then regulates a great number of downstream targets as indicated by the observed enrichment of transcription factor binding sites.…”

Section: Discussionmentioning

confidence: 99%

“…Animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Obihiro University (no. [18][19][20][21][22][23][24][25]. Twelve Holstein cows (3-7 yr of age) that had been ovariectomized 40 -50 days before the beginning of the experiment were maintained in a free-stall barn throughout the experimental period and had free access to food and water.…”

Section: Methodsmentioning

confidence: 99%

Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium

Shimizu

Krebs

Bauersachs

et al. 2010

Physiological Genomics

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The aim of our study was to analyze endometrial gene expression profiles in ovariectomized cows treated with estradiol and/or progesterone by using microarray analysis. Clustering of differentially expressed genes allowed separation into distinct hormone response patterns. These patterns could be classified into independent and interdependent actions of the steroid hormones estrogen and progesterone. The use of ovariectomized cows and external administration of hormones identified a set of genes whose regulation depends on a progesterone priming effect. The progesterone-primed estrogen response comprises gene functions such as migration, cell differentiation, and cell adhesion and therefore may play a crucial role in tissue remodeling, as one of its key regulators in the endometrium, TGFB2, is among this group of progesterone-primed genes. Functional annotation analysis of the estrogen-responsive gene clusters shows a clear dominance of functions such as cell cycle, morphogenesis, and differentiation. The functional profile of the progesterone-responsive clusters is less clear but nevertheless shows some important fertility-related terms like luteinization, oocyte maturation, and catecholamine metabolism. We looked for putative regulators of the steroid hormone response in endometrium by searching for enriched transcription factor binding sites in the promoter regions of the genes with similar hormone response profile. This analysis identified transcription factors such as SP1, NFYA, FOXA2, IRF2, ESR1, and NOBOX as candidate regulators of gene expression in bovine endometrium treated with steroid hormones. Taken together, our data provide novel insights into the regulation of bovine endometrial physiology by steroid hormones.

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“…Principles of animal care published by the National Laboratory Animal Center, National Science Council, Taiwan, were followed (Huang et al 2007). …”

Section: Animalsmentioning

confidence: 99%

Expression and in vitro regulation of pituitary adenylate cyclase-activating polypeptide (pacap38) and its type I receptor (pac1-r) in the gonads of tilapia (Oreochromis mossambicus)

Huang¹,

Li²,

Wu³

et al. 2009

Reproduction

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Pituitary adenylate cyclase-activating polypeptide (PACAP), a pleiotropic neuropeptide, has diverse functions in mammals. However, studies of the expression and function of PACAP and its receptor in fish, particularly in the reproductive system, are still limited. In this report, semi-quantitative RT-PCR and immunohistochemical staining were performed to identify expression domains of commercially important tilapia (Oreochromis mossambicus). PACAP (tpacap 38 ) and its type I receptor (tpac 1 -r). Transcripts were detected in the brain, gallbladder, gill, heart, intestine, kidney, muscles, pancreas, spleen, stomach, testes, and ovaries, but not in the liver. Expression of tpacap 38 and tpac 1 -r mRNA in brain tissue was significantly higher in both sexes compared with other tissues. Addition of exogenous ovine PACAP 38 (0.25-5 nM), cAMP analog (dibutyryl-cAMP, 0.25-1.5 mM) or forskolin (adenylate cyclase activator, 1-10 mM) significantly upregulated tpacap 38 in the gonads via a dose-and time-dependent fashion. This effect reached a maximal level at 2 h after induction, and then decreased with prolonged culture for up to 4 or 8 h. Additionally, the expression levels of tpac 1 -r were not significantly affected by ovine PACAP 38 or dibutyryl-cAMP in either sex. Forskolin had a slightly inductive effect and its function could be suppressed with the addition of protein kinase A (PKA) inhibitor, H89 (10 mM), indicating involvement of the cAMP-PKA signaling pathway in the regulation of tpacap 38 . Expression of tpacap 38 and tpac 1 -r in the gonads of tilapia suggests that PACAP may mediate gonadotropin action via paracrine/autocrine mechanisms in this bony fish.

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Steroid hormones (17β-estradiol and hydrocortisone) upregulate hepatocyte nuclear factor (HNF)-3β and insulin-like growth factors I and II expression in the gonads of tilapia (Oreochromis mossambicus) in vitro

Cited by 8 publications

References 52 publications

Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Glucocorticoid receptor activation following elevated oocyte cortisol content is associated with zygote activation, early embryo cell division, and IGF system gene responses in rainbow trout

Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium

Expression and in vitro regulation of pituitary adenylate cyclase-activating polypeptide (pacap38) and its type I receptor (pac1-r) in the gonads of tilapia (Oreochromis mossambicus)

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