Steroid hormones regulate cAMP and cGMP production by porcine granulosa cells in vitro

Effect of four cGMP analogues with different mechanisms of action on hormone release by porcine ovarian granulosa cells in vitro

“…Our observations confirm results of previous reports (Sirotkin and Nitray, 1993;Sirotkin et al, , 1998Danisova et al, 1995;Sirotkin, 1996;Masuda et al, 1997) with respect to the production of progesterone and oxytocin by cultured porcine granulosa cells. Some differences in basal hormone output observed in diferent experiments may be due to variability in material used (animals, progressing of cell luteinization a.o.…”

“…Steroid hormones can inhibit both, nitric oxide and cGMP production in the uterus (Yallampali et al, 1994). Furthermore, production of nitric oxide or cGMP by cultured ovarian cells can be stimulated by LH-RH, LH and FSH (Nitray et al, 1992;McGee et al, 1997;Kaipia and Hsueh, 1997;Tabruae et al, 1997), GH, prolactin , oxytocin (OT, Sirotkin et al, 1995;Sirotkin, 1996;Sirotkin et al, 1996), but not vasopressin or vasotocin (Nitray et al, 1992;Sirotkin et al, 1995), by steroid hormones (Sirotkin and Nitray, 1993;Sirotkin et al, 1995), melatonin (Sirotkin, 1994), tumor necrosis factor alpha (Brunswig-Spickenheier and Mukhopadhyay, 1997), interleukin-1 (Kaipia and Hsueh, 1997), atrial natriuretic factor (Pandey et al, 1987), and calcium ionophore (Danisova et al, 1995). Furthermore, cGMP analogues or activators of endogenous cGMP production are able to activate progesterone (P) and estradiol secretion (LaPolt and Hong, 1995;Masuda et al, 1997) and suppress apoptosis in ovarian cells (Kaipia and Hsueh, 1997;McGee et al, 1997).…”

Effects of prolactin on estrogen, cAMP and oxytocin secretion by porcine granulosa cells in vitro

“…The second step may be a cAMP-induced luteinization of the cell including specific changes in steroidogenesis (inhibition of estrogen and activation of progesterone secretion) and the stimulation of oxytocin release. On the other hand, a feedback effect of oxytocin and steroid hormones (Sirotkin and Nitray, 1993) on cAMP release, as well as a reciprocal influence of steroids on oxytocin (Wathes, 1989;Sirotkin and Nitray, 1992) can also occur.…”

Neural Cell Behavior and Fuzzy Logic

“…Neuropeptide Y treatment and food deprivation stimulate the cyclic AMP system [1124,224] and the effect is dependent upon the activation of the NMDA glutamate receptors [717] in the hypothalamus [898,174,717]. Cyclic AMP participations were also found for galanin, somatostatin, steroid sex hormones [1146], insulin [761], etc.…”

“…In multicellular animals, cyclic AMP is very important for neural function, but, for sure, in this case it is not concerned with mating only, although cyclic AMP is activated by steroid sex hormones [1146] and cyclic AMP pathways determine sexual differentiation of the brain. An example of this divergence is the response of cyclic AMP system to GABA.…”