Steroid measurement with LC–MS/MS. Application examples in pediatrics

Steroid profiling for congenital adrenal hyperplasia by tandem mass spectrometry as a second-tier test reduces follow-up burdens in a tertiary care hospital: A retrospective and prospective evaluation

“…By providing the result of second-tier test without delay, it can reduce unnecessary follow-up tests. Furthermore, inclusion of additional steroid hormones in the profiling panel, such as 11-deoxycortisol, 11-dehydrocorticosterone and corticosterone, would provide better discrimination between the CAH subtypes [12,14]. Our prospective data proved the clinical effectiveness of the steroid profiling test.…”

Standardized LC–MS/MS based steroid hormone profile-analysis

“…The modified online SPE-HPLC-MS/MS assay developed by Rauh et al, allowing quantitative analysis of steroid hormones in 100 L serum [2][3][4], was used as a reference HPLC-MS/MS assay for interassay comparison with the SteroIDQ ® kit. 11 steroid hormones (aldosterone, androstenedione, corticosterone, cortisol, cortisone, 11-deoxycortisol, DHEA, DHEAS, 17OHP, progesterone, and testosterone) were covered in this method.…”

“…Standardization is needed to minimize analytical intra-and inter-laboratory variabilities (imprecision and inaccuracy) and therefore, to improve the quality of the quantitative results [1,[3][4][5][6][7]9,13,15,18,23].…”

Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry: A novel approach for clinical monitoring and doping control

“…GC-MS/MS is widely used due to its high sensitivity and selectivity, but the derivatization procedure is complicated and sometimes the derivative efficiency is low. LC-MS/MS with relative simpler pretreatment, good sensitivity and selectivity receives analysts' favor especially in the analysis of low levels of AAS in biological samples [24]. The traditional pretreatment procedures, such as liquid-liquid extraction and off-line solid phase extraction, are not only time-consuming but also solvent and labor cost.…”