Steroid Metabolism in Theca Externa Cells from Preovulatory Follicles of Domestic Hen (Gallus domesticus)

Rodríguez, Enrique; Velázquez, P.N.; Juárez-Oropeza, Marco Antonio; Pedernera, E.

doi:10.1006/gcen.1996.0019

Cited by 22 publications

(6 citation statements)

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“…On the second day, the expression of CYP19A1 mRNA was significantly higher ( P <0.05) than at the other time points, and when combined with the high quantity of androgen synthesized on the first day, the results suggested that most of the androgen in theca externa cells was transformed into estrogen by the CYP19A1 protein on the second day. These steroid synthesis patterns and characteristics are consistent with previous reports [2,3,16,18,20], which indicate that the ability of theca cells to synthesize steroids in this culture model remained normal. After comprehensive analysis of theca cell density, viability, and assessment of their ability to synthesize steroids during d1–d7, we found that all parameters of cell density, viability, and expression of the CYP17A1/19A1 genes did not have a steady trend after seeding, which suggests that the latency period of theca cells should be between d0 and d1.…”

Section: Discussionsupporting

confidence: 92%

“…The viability of theca cells also remained high, which indicated that theca cells could maintain normal morphology and good activity in this cell culture model. The fact that the expression levels of CYP17A1/19A1 mRNA reached certain threshold values could reflect the steroid synthesis ability and viability of theca cells [3,18]. In our study, the expression of CYP17A1 mRNA was significantly higher on d1 ( P <0.05) than at other time points, which suggested that in vitro , androgen was synthesized in copious amounts from theca interna cells at the beginning of the culture.…”

Section: Discussionmentioning

confidence: 50%

“…Therefore, we cannot use RIA to authenticate avian theca cells. It has been reported that in hen follicular cells, the CYP17A1/19A1 protein exists specifically in theca cells (including theca interna cells and theca externa cells) on an organizational level [8,13,15,18]. Therefore, we used the CYP17A1/19A1 antibody to authenticate duck theca cells using the IF technique.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks

et al. 2017

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Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of the present study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence (IF) showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells. Furthermore, morphological differences were observed between the outlines of theca interna and externa cells and in their nuclei. Growth curve and CYP17A1/19A1 mRNA relative expression analyses suggested that the growth profile of theca interna cells may have been significantly different from that of theca externa cells in vitro. Theca interna cells experienced the logarithmic phase on d1–d2, the plateau phase on d2–d3, and the senescence phase after d3, while theca externa cells experienced the logarithmic phase on d1–d3, the plateau phase on d3–d5, and the senescence phase after d5. Taken together, these results suggested that we have successfully established a reliable theca cell culture model and further defined theca cell characteristics in vitro.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

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Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks

et al. 2017

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“…In the chicken ovary, the main source of progesterone is cells of the granulosa layer, whereas the main source of estradiol is cells of the theca layer [26,27]. The steroidogenic activity of a particular layer changes during the different physiological states of the ovary [28][29][30][31]. During maturation of yellow preovulatory follicles, the production of estrogens by the theca layer gradually decreases while synthesis of progesterone by the granulosa layer dramatically increases.…”

Section: Discussionmentioning

confidence: 99%

Comparative Changes in the Serum Concentrations of Inhibin-B, Prolactin, Gonadotropins and Steroid Hormones at Different Reproductive States in Domestic Turkey Hens

Khalil

Hanafy

Saleh

et al. 2009

Journal of Reproduction and Development

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Abstract. The present study was undertaken to compare the changes in circulating levels of inhibin-B, prolactin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol-17β, progesterone and testosterone during the different reproductive states of turkey hens. Blood samples were collected during different reproductive states, at laying, incubating and out of lay. Inhibin-B was measured by ELISA, while other hormones were determined by Chemiluminescent Microparticle Immunoassay (CMIA). The results revealed highly significant differences among the hen's states for all serum hormone concentrations. The highest levels of inhibin-B and prolactin were observed in incubating hens, while the lowest values were observed in laying hens. In contrast, the highest levels of FSH, LH, estradiol-17β, progesterone and testosterone were found in the laying group, while the lowest values were found in the incubating group. The progesterone level was higher in the laying group compared with the other groups. These results clearly demonstrate that negative correlation was found between both the inhibin-B and prolactin levels and the gonadotropin and steroid hormone concentrations during the different reproductive states of the turkey hens. In addition, the results suggest that inhibin-B may be involved in control of FSH and LH secretion. . The existence of bioactive inhibin in testis preparations [3,4] and ovarian granulosa cells [5,6] has been reported. The effect of induction of ovarian regression and removal of ovarian follicles on plasma inhibin indicate that the ovary is the major source of inhibin in the hen [7,8]. Prolactin secretion from the avian anterior pituitary gland is principally maintained by tonic stimulation from hypothalamic prolactinreleasing factors [9]. Prolactin secretion markedly changes during the reproductive cycle of the turkey hen. The hyperprolactinemia associated with incubation behavior (broodiness) induces ovarian regression, resulting in a substantial loss of egg production in commercial breeder flocks [10]. Administration of exogenous prolactin suppresses plasma gonadotropins necessary for egg production in domestic fowl [11]. However, there is additional evidence that suppression of gonadotropin secretion in incubating birds also involves a mechanism independent of increased prolactin secretion [12,13]. In turkey hens, the changes in the concentrations of inhibin-B, prolactin, gonadotropins and progesterone have been poorly documented. Therefore, in the present study, we investigated the changes in inhibin-B, prolactin, FSH, luteinizing hormone (LH), estradiol-17β, progesterone and testosterone hormones during the different reproductive states. Materials and Methods Animals and experimental designDomestic turkey hens (local Egyptian strain) were used in the present study. The turkeys were in the first year of production. The hens received a stimulatory photoperiod of 14 h of light:10 h of dark throughout the experimental period and were maintained in floor pens with trap nests. They...

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“…Blood samples were processed as in the previous experiment until assayed for estradiol and testosterone. The following organs were collected and frozen at −80°C until assayed for AA : the entire brain, portions of the pectoral muscle and of the thigh muscle, abdominal fat, heart, kidney, adrenals, small intestine, oviduct, and the ovarian follicle 3 of SHAM females (the 3 rd one in the laying sequence, which is particularly rich in aromatase; (Rodriguez-Maldonado et al, 1996)) that was drained of its yolk.…”

Section: Methodsmentioning

confidence: 99%

Non-ovarian aromatization is required to activate female sexual motivation in testosterone-treated ovariectomized quail

Bournonville

Balthazart

Ball

et al. 2016

Hormones and Behavior

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Although aromatase is expressed in both male and female brains, its functional significance in females remains poorly understood. In female quail, sexual receptivity is activated by estrogens. However it is not known whether sexual motivation is similarly estrogen-dependent and whether estrogens locally produced in the brain contribute to these behavioral responses. Four main experiments were designed to address these questions. In experiment 1 chronic treatment of females with the anti-estrogen tamoxifen decreased their receptivity, confirming that this response is under the control of estrogens. In experiment 2 chronic treatment with tamoxifen significantly decreased sexual motivation as treated females no longer approached a sexual partner. In experiment 3 (a) ovariectomy (OVX) induced a significant decrease of time spent near the male and a significantly decreased receptivity compared to gonadally intact females, (b) treatment with testosterone (OVX+T) partially restored these responses and (c) this effect of T was prevented when estradiol synthesis was inhibited by the potent aromatase inhibitor Vorozole (OVX+T+VOR). Serum estradiol concentration was significantly higher in OVX+T than in OVX or OVX+T+VOR females. Together these data demonstrate that treatment of OVX females with T increases sexual motivation and that these effects are mediated at least in part by non-gonadal aromatization of the androgen. Finally, assays of aromatase activity on brain and peripheral tissues (Experiment 4) strongly suggest that brain aromatization contributes to behavioral effects observed here following T treatment but alternative sources of estrogens (e.g. liver) should also be considered.

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Steroid Metabolism in Theca Externa Cells from Preovulatory Follicles of Domestic Hen (Gallus domesticus)

Cited by 22 publications

References 0 publications

Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks

Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks

Comparative Changes in the Serum Concentrations of Inhibin-B, Prolactin, Gonadotropins and Steroid Hormones at Different Reproductive States in Domestic Turkey Hens

Non-ovarian aromatization is required to activate female sexual motivation in testosterone-treated ovariectomized quail

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