Steroid receptors in blood vessels of the rhesus macaque endometrium: a review

Brenner, Robert; Slayden, Ov D.

doi:10.1679/aohc.67.411

Cited by 33 publications

(27 citation statements)

References 35 publications

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“…15 In this study, HEECs' tube formation was negatively affected when HEECs had grown together with stromal cells and exposed to mifepristone, indicating similar occurrence as Brenner and Slayden saw in nonhuman primates. 29 The HEECs exposed to 100 mmol/L mifepristone showed heavily reduced proliferation and viability rates. This concentration was therefore considered as cytotoxic and was not further investigated.…”

Section: Discussionmentioning

confidence: 95%

Mifepristone-Exposured Human Endometrial Endothelial Cells In Vitro

et al. 2014

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The antiprogestin mifepristone has been used for more than 20 years as a medical alternative for early pregnancy termination. After mifepristone administration, significant changes have been observed in the endometrial vessels, with cell injury and cell death in capillary endothelial cells. In this study, the effect of mifepristone on human endometrial endothelial cells (HEECs) in vitro was evaluated using proliferation and viability assays, quantitative polymerase chain reaction of markers important for the regulation of angiogenesis, and by tube formation assay. There were no detectable effects of mifepristone on HEECs messenger RNA expression of the studied markers. Exposure to mifepristone did not alter tube formation. However, mifepristone exposure to HEECs cocultured with endometrial stromal cells significantly reduced the activity in the tube formation assay compared with mifepristone exposure of HEECs in monoculture. This implies that mifepristone causes changes in HEEC-associated angiogenic activity and that this effect is mediated through stromal cells via paracrine mechanisms.

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Section: Discussionmentioning

confidence: 95%

Mifepristone-Exposured Human Endometrial Endothelial Cells In Vitro

et al. 2014

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“…Vascular proliferation in the spiral arteries, which is normally elevated in the luteal phase due to high serum P [20] was characteristically high in animals treated with the blank IUD, but was dramatically inhibited in animals treated with AP-IUDs (Fig. 8). 3.3.2.…”

Section: Effects On Endometrial Histology and Steroid Receptor Immunomentioning

confidence: 97%

Antiprogestin-releasing intrauterine devices: a novel approach to endometrial contraception

et al. 2007

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Intrauterine devices (IUDs) that release progestins are highly effective contraceptives, but they induce breakthrough bleeding that some women find unacceptable. Because progesterone (P) antagonists (AP) are known to suppress the endometrium, induce amenorrhea, and inhibit fertility, AP IUDs may provide an effective contraceptive that also controls endometrial bleeding. Here we assessed the effects of empty (blank) vs AP-releasing (ZK 230 211) IUDs on bleeding patterns and endometrial growth in ovariectomized, artificially cycled macaques. The AP IUDs (but not the blank controls) induced extended, frank menstruation when inserted during the late luteal phase, an indication of local AP action. Over time, endometrial glandular and arterial proliferation were inhibited, steroid receptors were elevated, spiral arteries showed degenerative changes, progesterone withdrawal bleeding was prevented and estradiol-dependent proliferation was suppressed by the AP IUDs. In sum, AP IUDs suppressed the effects of P on endometrial progestational development and blocked the effects of estradiol on endometrial proliferation as previously shown for systemic treatment with APs. Therefore, AP IUDs may provide novel contraceptive devices with minimal breakthrough bleeding.

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“…A distinctive feature of endometrial endothelial cells of the marmoset was the preferential staining for ERb over PR or ERa at all stages of the cycle and during pregnancy. This phenomenon has been described in women (Critchley et al 2001, Lecce et al 2001) and macaques (Brenner & Slayden 2004), and has been confirmed by studies on human microvascular endometrial endothelial cell (HEEC) cultures (Krikun et al 2005). This suggests a role for oestrogen directly upon endometrial Oestrogen and progesterone receptors in marmoset endothelial cells influencing angiogenesis and vascular permeability.…”

Section: Discussionmentioning

confidence: 56%

Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy

Silvestri

Fraser

2007

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Marmosets are widely used, but detailed studies on localisation of endometrial oestrogen receptors a and b (ERa and ERb), and the progesterone receptor (PR) are lacking. These receptors were localised and semi-quantitatively analysed throughout the ovulatory cycle, weeks 2, 3 and 4 of pregnancy and after treatment with GnRH antagonist, vascular endothelial growth factor (VEGF) Trap or ovariectomy. The PR in epithelial cells increased markedly between the mid-and late proliferative phases before declining in the mid-secretory phase and pregnancy. PR in stromal cells was present throughout the cycle and levels were maintained in pregnancy. ERa was present at the mid-proliferative phase and increased in glands at the late proliferative and early secretory phases, before declining at the late secretory phase and week 4 of pregnancy. Stromal ERa showed a similar trend, but decreased earlier, by the mid-secretory phase. ERb was highly expressed in epithelial cells throughout the cycle and in pregnancy. In stroma, increases in ERb expression were observed at the late proliferative phase with the staining index decreasing by half as the secretory phase progressed and in pregnancy. GnRH antagonist, VEGF Trap or ovariectomy caused significant reductions in PR and ERb expression, but not in ERa when compared with the late proliferative phase of the normal cycle. Endothelial cells expressed ERb, but not ERa or PR. It is concluded that the steroid receptor profile in the marmoset endometrium is generally similar to the human and should provide a useful model for studies on hormonal manipulation of the endometrium.

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Steroid receptors in blood vessels of the rhesus macaque endometrium: a review

Cited by 33 publications

References 35 publications

Mifepristone-Exposured Human Endometrial Endothelial Cells In Vitro

Mifepristone-Exposured Human Endometrial Endothelial Cells In Vitro

Antiprogestin-releasing intrauterine devices: a novel approach to endometrial contraception

Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy

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