Steroidal Saponins from Polygonatum cyrtonema

Ma, Ke; Huang, Xuefeng; Kong, Ling–Yi

doi:10.1007/s10600-013-0770-2

Cited by 23 publications

(7 citation statements)

References 9 publications

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“…Therefore, peaks 13, 14, and 17 were tentatively identified as (25 S )‐spirostan‐5‐en‐12‐one‐3‐ O ‐ β ‐D‐glucopyranosyl‐(1→2)‐ O ‐[ β ‐D‐glucopyranosyl‐(1→3)]‐ O ‐ β ‐D‐glucopyranosyl‐(1→4)‐ β ‐D‐galactopyranoside or its isomer, (25 S )‐spirostan‐5‐en‐12‐one‐3‐ O ‐ β ‐D‐glucopyranosyl‐(1→2)‐ O ‐[ β ‐D‐glucopyranosyl‐(1→3)]‐ O ‐ β ‐D‐glucopyranosyl‐(1→4)‐ β ‐D‐galactopyranoside or cyrtonemoside A, and cyrtonemoside A (Figure S7B) or its isomer, respectively. They were in accordance with previous literature data [3].…”

Section: Resultssupporting

confidence: 93%

“…The rhizome of PCH, also named ‘Huang Jing’, can nourish Yin and reinforce Qi, strengthening the spleen, lungs, and kidneys [2]. According to modern pharmacology and clinical studies, the potential health benefits of PCH may be attributed to its bioactive components such as steroidal saponins [3], polysaccharides [4], and flavonoids [5]. Steroidal saponins are characterized by a skeleton derived from oxidosqualene, consisting of a sugar moiety linked to a steroidal (27 carbon atoms) aglycone [6].…”

Section: Introductionmentioning

confidence: 99%

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Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

Liu

Feng

Sui

et al. 2023

J of Separation Science

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In this study, quartz sand with particularly sharp nanoscale edges acted like a nanoscale knife physically cut cells of Polygonatum cyrtonema Hua into nanosized particles and was synergized with natural deep eutectic solvent to extract steroidal saponins of Polygonatum cyrtonema Hua. The natural deep eutectic solvent (choline chloride‐lactic acid)‐synergistic quartz sand‐assisted extraction was optimized using response surface methodology. The steroidal saponins purified with AB‐8 macroporous resin were identified using ultra‐high‐performance liquid chromatography‐triple time of flight mass spectrometry. The results showed that the experimental total saponins content value (36.97 ± 0.12 mg dioscin equivalent/g dry weight) at optimal extraction conditions with a temperature of 68°C, a rotational speed of 20 400 rpm, shear time of 4.3 min, the liquid‐solid ratio of 38 ml/g, was close to the maximum possible theoretical value (36.64 mg dioscin equivalent/g dry weight). A total of 20 steroidal saponins were identified, among which the content of (25R)‐Kingianoside E was the highest (102.66 ± 3.47 mg/g). Furthermore, a new steroid saponin (3β,25S)‐26‐(β‐D‐glucopyranosyloxy)‐22‐hydroxyfurost‐5‐en‐3‐yl 4‐O‐β‐D‐glucopyranosyl‐β‐D‐galactopyranoside+Glc was found for the first time. These results revealed that natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction was an efficient and green method to extract a variety of steroidal saponins.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

Liu

Feng

Sui

et al. 2023

J of Separation Science

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“…According to the discrepant fragments at m / z 915.4616 and 1047.5086, compound # 56 exhibited NL of 162 Da from m / z 915 to 753, while compound # 62 lost Gal and Xyl successively based on the transition from m / z 1047 to 753. They were finally speculated to be (25 S )-pratioside D1 or its isomer and spirost-5-en-12-one-3-O- β - d -glucopyranosyl-(1→2)-[ β - d -xylopyranosyl-(1→3)]- β - d -glucopyranosyl-(1→4)- β - d -galactopyranoside or its isomer [ 42 , 43 ].…”

Section: Resultsmentioning

confidence: 99%

Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole–Linear Ion Trap Mass Spectrometry

et al. 2022

Molecules

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Wenxin granule (WXG) is a popular traditional Chinese medicine (TCM) preparation for the treatment of arrhythmia disease. Potent analytical technologies are needed to elucidate its chemical composition and assess the quality differences among multibatch samples. In this work, both a multicomponent characterization and quantitative assay of WXG were conducted using two liquid chromatography–mass spectrometry (LC-MS) approaches. An ultra-high performance liquid chromatography–ion mobility quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) approach combined with intelligent peak annotation workflows was developed to characterize the multicomponents of WXG. A hybrid scan approach enabling alternative data-independent and data-dependent acquisitions was established. We characterized 205 components, including 92 ginsenosides, 53 steroidal saponins, 14 alkaloids, and 46 others. Moreover, an optimized scheduled multiple reaction monitoring (sMRM) method was elaborated, targeting 24 compounds of WXG via ultra-high performance liquid chromatography–triple quadrupole linear ion trap mass spectrometry (UHPLC/QTrap-MS), which was validated based on its selectivity, precision, stability, repeatability, linearity, sensitivity, recovery, and matrix effect. By applying this method to 27 batches of WXG samples, the content variations of multiple markers from Notoginseng Radix et Rhizoma (21) and Codonopsis Radix (3) were depicted. Conclusively, we achieved the comprehensive multicomponent characterization and holistic quality assessment of WXG by targeting the non-volatile components.

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“…2. A total of 65 active components for AD treatment were identified using DS [Table SI (22,23,(45)(46)(47)(48)(49)(50)(51)(52)].…”

Section: Identification Of the Main Active Components Of Erjing Pillmentioning

confidence: 99%

Evidence‑based complementary and alternative medicine bioinformatics approach through network pharmacology and molecular docking to determine the molecular mechanisms of Erjing pill in Alzheimer's disease

et al. 2021

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Erjing pill, a Traditional Chinese Medicine (TCM) formulation composed of Polygonatum sibiricum and Lycium chinense, has an important role in the treatment of Alzheimer's disease (AD). However, the underlying mechanisms of the action of Erjing pill in AD have remained elusive. In the present study, the key ingredients of Erjing pill were investigated and the active components and their mechanisms of action on AD were analyzed based on networks pharmacology. By using the TCM and TCM Systems Pharmacology and databases, the components of Erjing pill were screened and the data were captured using Discovery Studio. The SwissTarget webserver database was used to predict the potential protein targets of Erjing pill components for pathologies related to AD. The data were further analyzed with the disease targets of AD based on analysis of the Online Mendelian Inheritance in Man, DiGSeE and Therapeutic Target Database. Subsequent analysis of mechanistic pathways of the screened components and protein targets allowed us to construct a network by using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, which revealed potential molecular mechanisms of Erjing pill against AD. Finally, the protective effect of three active components on neurons was verified using an in vitro injury model of PC12 cells induced by Aβ 25-35 . The results indicated that 65 bioactive components of Erjing pill, including lauric acid and zederone, and 6 targets, including acetylcholinesterase, butylcholinesterase and amyloid protein precursor, were closely associated with the prevention and treatment of AD. The molecular components of Erjing pill were indicated to be involved in various biological signaling processes, mainly in synaptic signal transmission, transsynaptic signal transmission and chemical synaptic transmission. Furthermore, related pathways targeted by Erjing pill in AD included the regulation of neuroactive ligand-receptor interactions, the PI3K-Akt signaling pathway, serotoninergic synapses, calcium signaling pathways and dopaminergic synapses. A cell viability assay indicated that the compounds (polygonatine A, polygonatine C and 4' ,5-dihydroxyflavone) assessed were able to significantly improve the survival rate and increase the Ca 2+ level in a PC12 cell model of AD induced by amyloid-β 25-35 . The present study revealed that the mechanisms of action of Erjing pill to prevent and treat AD included a multicompound, multitarget and multipathway regulatory network.

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Steroidal Saponins from Polygonatum cyrtonema

Cited by 23 publications

References 9 publications

Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

Extraction and identification of steroidal saponins from Polygonatum cyrtonema Hua using natural deep eutectic solvent‐synergistic quartz sand‐assisted extraction method

Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole–Linear Ion Trap Mass Spectrometry

Evidence‑based complementary and alternative medicine bioinformatics approach through network pharmacology and molecular docking to determine the molecular mechanisms of Erjing pill in Alzheimer's disease

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