Steroidal saponins from Raphia vinifera and their cytotoxic activity

Fru, Godloves; Sop, Rodrigue V. Tchangna; Mbaveng, Armelle T.; Ombito, Japheth O.; Fotso, Ghislain Wabo; Nguenang, Gaëlle S.; Kuete, Victor; Efferth, Thomas; Ngadjui, Bonaventure T.

doi:10.1016/j.steroids.2020.108724

Cited by 13 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those activities are due to different chemical compounds present in the plant extract. This result is in agreement with those of some authors [ 18 , 26 ], which have shown that the Raphia vinifera compounds have cytotoxicity activity against cancer cell lines. Previous research showed that saponins from Raphia vinifera (Progenin III) induced necroptosis, autophagy, and apoptosis in leukemia cells [ 26 ].…”

Section: Discussionsupporting

confidence: 94%

“…The NMR spectra and the chemical shifts of compounds 1–9 are provided in the Supplementary file ( S1 to S18 ). They are isolated from Raphia vinifera fruits collected in Bambili, Northwest Region of Cameroon on April 2016, and identified at the Cameroon National Herbarium (voucher number: 38374/HNC) as previously reported [ 18 ]. Doxorubicin 98.0% (Sigma-Aldrich) (Munich, Germany) comes from the Medical Center of the Johannes Gutenberg University (Mainz, Germany) and is dissolved in phosphate buffer saline (PBS; Invitrogen, Eggenstein, Germany) at 10 mM.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Botanical from the Fruits Mesocarp of Raphia vinifera Displays Antiproliferative Activity and Is Harmless as Evidenced by Toxicological Assessments

Nguenang

Mbaveng

Bonsou

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Raphia vinifera is widely used to treat several diseases including digestive disorders, dysentery, and genitourinary infections. In this study, the mineral contents, the cytotoxicity, and the toxicological effect of the crude CHCl3/MeOH extract (RVM) from the mesocarp of Raphia vinifera were evaluated. The mineral contents were evaluated using the method described by the Association of Official Analytical Chemists (AOAC). The cytotoxicity of both extract and chemical compounds from the plants was determined by a resazurin reduction assay (RRA). The toxicological studies were carried out using the experimental procedure of the Organization for Economic Cooperation and Development (OECD). After killing the rats, biochemical, histopathological, and hematological studies were performed. The result indicated that RVM is rich in zinc (6.52 mg/100 g of DM) and sodium (194.5 mg/100 g of DM). RVM had a cytotoxicity effect with IC50 values lower than 30 μg/mL in 18/18 cancer cell lines tested. These recorded IC50 values were between 12.35 µg/mL (toward CCRF-CEM leukemia cells) and 26.66 µg/mL (toward SKMel-505 BRAF wild-type melanoma cells). Raphvinin 4 displayed good cytotoxicity against MaMel-80aBRAF-V600E homozygous mutant with the IC50 of 10.42 μM. RVM was relatively nontoxic to rats, the median lethal dose (DL50) being above 5000 mg/kg body weight. However, during the oral administration period extending for 28 days, precautions should be taken due to the increase in urinary creatinine level and decrease in spleen weight in the male rats given the highest dose (1000 mg/kg) of extract. Conclusively, the extract of Raphia vinifera is weakly toxic in rats and could be further used in the development of anticancer phytomedicines.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Botanical from the Fruits Mesocarp of Raphia vinifera Displays Antiproliferative Activity and Is Harmless as Evidenced by Toxicological Assessments

Nguenang

Mbaveng

Bonsou

et al. 2022

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chi (Chi et al, 2020) isolated 4 saponins from Raphia vinifera, being toxic in average concentrations of 3.55 to 7.14 µM in cell lines sensitive to drugs CCRF-CEM, whereas in cell lines resistant to drugs, CEM/ADR5000 concentration mean ranged from 9.19 to 12.29 µM, showing an anticancer potential, since the cell lines tested are leukemic cells. The saponins present in this species induced a cytotoxic effect in a drug-resistant phenotype, showing a potential alternative in the treatment of cancer unresponsive to conventional drugs.…”

Section: Cytotoxicity and Anticancermentioning

confidence: 99%

Emerging source of bioactive compounds from Arecaceae family: a systematic review

Silva

Miranda

Amorim

et al. 2021

RSD

View full text Add to dashboard Cite

The use of plants for medicinal purposes is performed empirically by traditional knowledge with the use of preparations that seek to extract their active principles, and are considered to be fundamental to human health. In this context, the aim of the present study was to of this study was to carry out a systematic review of the biological activities of the Arecaceae family distributed throughout Brazil. This research was carried out through a comprehensive search using the following databases, Scopus, Portal Periódicos Capes, PubMed, Google Scholar and Science Direct, using the following descriptors: “Arecaceae” and “Biological properties of the family Arecaeae”, checked at <www.theplantlist.org/> to check synonyms. It was possible to identify numerous biological activities in the arecaceae family, among the most recurring ones, the antioxidant, antimicrobial and anti-inflammatory activity. These activities are justified by the presence of fatty acids, phenolic compounds, alkaloids and terpenes. Studies routinely report lauric acid as a major in the plants of this family, which makes it a potential compound to cure or assist in the treatment of various diseases.

show abstract

“…The group of compounds mostly identified in that fraction are steroid compounds. Steroid compounds have been widely studied to have cytotoxic activity [27][28][29] . Gamma-sitosterol is a steroid compound that is mostly found in the n-hexane fraction.…”

Section: Molecular Dockingmentioning

confidence: 99%

Sterculia foetida Leaf Fraction Against Matrix Metalloproteinase-9 Protein and 4T1 Breast Cancer Cells: In-Vitro and In-Silico Studies

2021

TJNPR

View full text Add to dashboard Cite

expression of MMP-9 can change due to genetic variations that affect the effectiveness of breast cancer treatment. 9 There has been many studies proving that chemical compounds in plants have cytotoxic activity. This is due to variations in the structures of the chemical compounds and the mechanisms for causing different cytotoxic effects. 10 This has led many researchers to conduct research to explore plants and chemical compounds that have anticancer effects. 11 S. foetida is a medicinal plant that has many pharmacological activities. 12 Mujumdar et al. 13 reported that the leaves of S. foetida contain luteolin, α-sitosterol, scutellarein, taraxerol, noctacosanol, and procyanidin, which have analgesic and antiinflammatory activity. Rajasekharreddy and Rani 14 reported finding cyclopropene fatty acid compounds, such as (2n-octylcycloprop-1enyl)-octanoic acid (I) from S. foetida seeds which have antibacterial, antiviral, and cytotoxic activities. In this study, we explored S. foetida leaves fractions of n-hexane, chloroform, ethyl acetate n-butanol, and insoluble n-butanol as a possible source of compounds that have cytotoxic activities. The in vitro inhibitory activities of these fractions against MMP-9 protein and 4T1 cancer cells were further studied. The compounds contained in the most active fraction were identified using Gas Chromatography-Mass Spectroscopy (GC-MS). The interaction of the compounds in the most active fraction against a protein target, MMP-9 involved in cancer pathogenesis was analyzed through molecular docking techniques.

show abstract

Steroidal saponins from Raphia vinifera and their cytotoxic activity

Cited by 13 publications

References 23 publications

Botanical from the Fruits Mesocarp of Raphia vinifera Displays Antiproliferative Activity and Is Harmless as Evidenced by Toxicological Assessments

Botanical from the Fruits Mesocarp of Raphia vinifera Displays Antiproliferative Activity and Is Harmless as Evidenced by Toxicological Assessments

Emerging source of bioactive compounds from Arecaceae family: a systematic review

Sterculia foetida Leaf Fraction Against Matrix Metalloproteinase-9 Protein and 4T1 Breast Cancer Cells: In-Vitro and In-Silico Studies

Contact Info

Product

Resources

About