Steroidal saponins from the roots of Trillium erectum (Beth root)

Hayes, Peter C.; Lehmann, R.; Penman, Kerry G.; Kitching, William; Voss, James J. De

doi:10.1016/j.phytochem.2008.10.019

Cited by 55 publications

(64 citation statements)

References 22 publications

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“…Protein expression was determined by western blot analysis. Briefly, Rh1 cells (2x10 5 ) were seeded in six-well plates for 12 h and then treated with the indicated concentrations of TTB2 for 24 h. Following separation by SDS-PAGE, the proteins were transferred to polyvinylidene difluoride membranes and subjected to immunoblotting with antibodies against p-ERK, ERK, p-Akt [serine (Ser)473], Akt and tubulin at 4˚C overnight. Subsequent to washing with 5% skimmed milk in tris-buffered saline and Tween 20 (TBST) buffer (5 mM Tris-HCl, pH 7.6, 136 mM NaCl and 0.05% Tween-20), the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and visualized using the enhanced chemiluminescence system (Pierce, Rockford, IL, USA).…”

Section: Plant Materials and Extraction And Isolation Of Ttb2mentioning

confidence: 99%

“…Trillium tschonoskii Maxim, also known as 'a pearl on head', is predominantly distributed in mid-Western China and has been used in traditional remedies for the treatment of headache, hypertension, neurasthenia, giddiness and cancer, as well as for the removal of carbuncles and the amelioration of pain, for ≥1,000 years (3,4). Previous studies have shown that a number of bioactive components, such as steroidal saponins and glycosides, can be found in species of the Trillium genus, including T. erectum (5,6), T. kamtschaticum (7,8) and T. tschonoskii Maxim (9). Saponins are identified by their structures which contain a steroidal or triterpenoid aglycone and one or more sugar chains (10).…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Huang

Zou

2015

Experimental and Therapeutic Medicine

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Abstract. The steroidal saponin TTB2 can be isolated from the n-BuOH extracts of Trillium tschonoskii Maxim. The aim of the present study was to observe whether this saponin exerted any cytotoxic effects on malignant sarcoma cells, and to further investigate the possible underlying molecular mechanisms. The cell viability, cell cycle arrest and phosphorylation of certain important signal molecules in the sarcoma cell line were investigated. It was found that TTB2 inhibited the growth of the Ewing sarcoma cell line and arrested cells in the G 2 /M and S phases of the cell cycle in a dose-and time-dependent manner. Furthermore, the phosphorylation of extracellular signal-regulated kinase was inhibited by TTB2. In conclusion, the results showed that the saponin TTB2 isolated from T. tschonoskii Maxim exerts anticancer effects and may be a potential candidate for the development of anticancer drugs for use in the treatment of cancer.

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Section: Plant Materials and Extraction And Isolation Of Ttb2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Huang

Zou

2015

Experimental and Therapeutic Medicine

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“…[15] Steroidal saponins are common constituents of a variety of commercially important herbal medicines. [16,17] They consist of a steroidal nucleus, commonly an oxidised cholesterol derivative, with a number of sugar moieties at varying positions. The presence of a hydroxyl moiety at C-16 and a carbonyl at C-22 yields the hemiacetal found in furostanol saponins, and an additional free hydroxyl at C-26 results in the formation of a bicyclic acetal characteristic of spirostanol saponins; these two motifs are the most common structures found in steroidal saponins.…”

Section: Introductionmentioning

confidence: 99%

The Truth about False Unicorn (Chamaelirium luteum): Total Synthesis of 23R,24S‐Chiograsterol B Defines the Structure and Stereochemistry of the Major Saponins from this Medicinal Herb

Matovic

Stuthe

Challinor

et al. 2011

Chemistry A European J

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Chamaelirium luteum is used in traditional medicine systems and commercial botanical dietary supplements for the treatment of female reproductive health problems. Despite the wide use of this herb, only very limited phytochemical characterisation is available. Our investigation of C. luteum roots led to the isolation of two new steroidal saponins 1 and 2 that contain an unusual aglycone 3. The absolute configurations of these molecules were unable to be determined spectroscopically and thus the total synthesis of 3 was undertaken and achieved in 16 steps and 1.6 % overall yield from pregnenolone. The key step in the synthesis was the stereoselective installation of the side chain at C-17 and C-20, which employed anion-accelerated oxy-Cope methodology. The relative configuration of aglycone 3 was determined by X-ray crystallography of an advanced synthetic intermediate. The absolute configuration was based upon that of the pregnenolone-derived steroidal skeleton and determined to be 23R,24S.

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“…Some T. erectum extracts exhibited a moderate activity against HSV-1 and steroidal saponins and glycosides were isolated from this plant and could explain activity (Hayes et al, 2009;. Khan et al (2005) reported that these compounds showed anti-HSV activity.…”

Section: Discussionmentioning

confidence: 96%

Évaluation de l'activité antivirale de cinq espèces végétales de la forêt boréale contre les virus influenza de type A et l'herpès simplex de type 1 /

Côté¹

2011

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RÉSUMÉL'action directe des extraits sur les virus a également été testée. Finalement, la cytotoxicité des extraits a été déterminée sur des fibroblastes de peau humaine en culture. IllLes résultats ont montré que la plupart des extraits possédaient une activité modérée contre Y influenza. La plante ayant obtenue la meilleure activité est Cornus canadensis avec une inhibition de 16 à 96% (selon le moment de l'infection) à une concentration de 100|Lig/ml. D'autre part, cette plante a également été trouvée fortement active contre le virus de l'herpès simplex de type 1. Les extraits de feuilles ont non seulement une activité antivirale directe sur le virus (IC50 =11 |ig/ml) mais inhibent aussi sa replication (IC50 = 44 |ug/ml). L'ensemble des résultats obtenus in vitro confirme l'activité antivirale contre le virus de 1'influenza de plusieurs plantes utilisées en médecine traditionnelle amérindienne et montre pour la première fois une activité contre le virus de l'herpès simplex type 1. Park and Fowler, 1989;Turner and Efrat, 1982) Décoction : toux, rhume et fièvre 18 Traitements et résistances Description des plantesLes cinq plantes sélectionnées pour ce projet sont présentes dans la forêt boréale québécoise. Une description sommaire des plantes est présentée ici suivi d'une photo de chacune de ces plantes (voir figure 5) (Marie-Victorin, 1995). UAchillea millefolium, ou For inhibition of HSV-1 replication, G canadensis extracts exhibited also the higher activity with IC50 values ranging from 44 to 69 |ig/ml. Finally, water/ethanol decoction of A. millefolium leaf was found the most active extract to inhibit directly HSV-1 with an IC50 29 value of 4.8 ± 0.6 |ig/ml. Altogether, the results presented in this work support antiviral activity of some plants used in Native American traditional medicine. 30

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Steroidal saponins from the roots of Trillium erectum (Beth root)

Cited by 55 publications

References 22 publications

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

The Truth about False Unicorn (Chamaelirium luteum): Total Synthesis of 23R,24S‐Chiograsterol B Defines the Structure and Stereochemistry of the Major Saponins from this Medicinal Herb

Évaluation de l'activité antivirale de cinq espèces végétales de la forêt boréale contre les virus influenza de type A et l'herpès simplex de type 1 /

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