Steroidal saponins with cytotoxic activities from the rhizomes of Anemarrhena asphodeloids Bge.

Zhang, Yu-Wei; Zhao, Yunfang; Wang, Yin-Ru; Shang, T M; Yang, Lei-Min; Huang, Xuefeng

doi:10.1016/j.phytol.2017.04.013

Cited by 6 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ZM 20141020) was deposited in Department of Natural Medicinal Chemistry, China Pharmaceutical University. TAIII was isolated from A. asphodeloides according to the protocols reported previously ( Wang et al, 2017 ; Zhang et al, 2017 ). TAIII (purity >98%) was dissolved in DMSO to 10 mM as a stock solution and stored at −20°C.…”

Section: Methodsmentioning

confidence: 99%

Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways

Zhang

Gao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Timosaponin AIII (TAIII), a steroidal saponin, exerts potent anti-tumor activity in various cancers, especially breast cancer. However, the concrete molecular mechanisms of TAIII against breast cancer are still unclear. Here, we find that TAIII triggers DNA damage, leads to G2/M arrest, and ultimately induces apoptosis in breast cancer both in vitro and in vivo. TAIII induced G2/M phase arrest and apoptosis in MDA-MB-231 and MCF7 cells accompanied with down-regulation of CyclinB1, Cdc2 and Cdc25C. Further data showed that the ATM/Chk2 and p38 pathways were activated representing by up-regulated levels of p-H2A.X and p-p38, which indicated an induction of DNA damage by TAIII, leading to cell cycle arrest and apoptosis. The effects of TAIII were further confirmed by employing inhibitors of ATM and p38 pathways. In vivo, TAIII suppressed the growth of subcutaneous xenograft tumor without obvious toxicity, which indicated by Ki67 and TUNEL analysis. Data also showed that TAIII stimulated the ATM/Chk2 and p38 MAPK pathways in vivo, which in consistent with the effects in vitro. Hence, our data demonstrate that TAIII triggers DNA damage and activates ATM/Chk2 and p38 MAPK pathways, and then induces G2/M phase arrest and apoptosis in breast cancer, which provide theoretical evidence for TAIII utilized as drug against breast cancer.

show abstract

Section: Methodsmentioning

confidence: 99%

Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways

Zhang

Gao

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Anemarrhenae rhizome, the rhizome of Anemarrhena asphodeloides Bunge ( Chinese Pharmacopoeia edition), has been extensively used for the clinical treatment of febrile diseases, diabetes, senile dementia and cancer (Fu, Li, Xue, Jiang, & Huang, ; Takeda et al, ; Yang, Liu, Hu, Lai, & Xia, ). Pharmacological studies have confirmed a panel of bioactivities such as anti‐inflammation, anti‐platelet aggregation, anti‐diabetic, anti‐depression, anti‐tumour and inhibitory effects on β‐amyloid (Khang, Phuong, & Ma, ; Zhang et al, ). A. asphodeloides mainly contains steroidal saponins, including timosaponin B‐II and timosaponin A‐III (Zhang et al, ).…”

Section: Introductionmentioning

confidence: 98%

A validated UPLC–MS/MS method for quantitative determination of a potent neuroprotective agent Sarsasapogenin‐AA13 in rat plasma: Application to pharmacokinetic studies

Pei

Zhao

et al. 2020

Biomedical Chromatography

View full text Add to dashboard Cite

Sarsasapogenin‐AA13(AA13), a sarsasapogenin derivative, exhibited good neuroprotective and anti‐inflammatory activities in vitro and therapeutic effects on learning and memory dysfunction in amyloid‐β‐injected mice. A sensitive UPLC–MS/MS method was developed and validated to quantitatively determine AA13 in rat plasma and was further applied to evaluate the pharmacokinetic behaviour of AA13 in rats that were administered AA13 intravenously and orally. This method was validated to exhibit excellent linearity in the concentration range of 1–1000 ng/mL. The lower limit of quantification was 1 ng/mL for AA13 in rat plasma. Intra‐day accuracy for AA13 was in the range of 90–114%, and inter‐day accuracy was in the range of 97–103 %. The relative standard deviation of intra‐day and inter‐day assay was less than 15%. After a single oral administration of AA13 at the dose of 25 mg/kg, Cmax of AA13 was 1266.4 ± 316.1 ng/mL. AUC0–48 h was 6928.5 ± 1990.1 h·ng/mL, and t1/2 was 10.2 ± 0.8 h. Under intravenous administration of AA13 at a dosage of 250 μg/kg, AUC0–48 h was 785.7 ± 103.3 h⋅ng/mL, and t1/2 was 20.8 ± 7.2 h. Based on the results, oral bioavailability (F %) of AA13 in rats at 25 mg/kg was 8.82 %.

show abstract

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

et al. 2020

View full text Add to dashboard Cite

Steroidal saponins are a group of glycosides widely distributed among monocotyledonous families. They exert a wide spectrum of biological effects including cytotoxic and antitumor properties which are the most studied. This review is an update of our previous paper-Saponins as cytotoxic agents (Podolak et al. in Phytochem Rev 9:425-474, 2010) and covers studies that were since published (2010-2018). In this paper we refer to steroidal saponins presenting results of cytotoxicity studies, mechanisms of action and structure-activity relationships.

show abstract

Steroidal saponins with cytotoxic activities from the rhizomes of Anemarrhena asphodeloids Bge.

Cited by 6 publications

References 11 publications

Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways

Timosaponin AIII Induces G2/M Arrest and Apoptosis in Breast Cancer by Activating the ATM/Chk2 and p38 MAPK Signaling Pathways

A validated UPLC–MS/MS method for quantitative determination of a potent neuroprotective agent Sarsasapogenin‐AA13 in rat plasma: Application to pharmacokinetic studies

Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins

Contact Info

Product

Resources

About