Steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium

Fahrbach, Michael; Kuever, Jan; Remesch, Markko; Huber, Birgit; Kämpfer, Peter; Dott, Wolfgang; Hollender, Juliane

doi:10.1099/ijs.0.65342-0

Cited by 185 publications

(90 citation statements)

References 49 publications

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“…This observation is reflected by the inability of Sdo. denitrificans to grow on cyclohexanol (Fahrbach et al, 2008). We observed a temporal increase in atcA PCR products during the denitrifying incubation of sewage samples with testosterone.…”

Section: Discussionmentioning

confidence: 69%

“…denitrificans is its narrow substrate spectrum, which is restricted to a few steroids (natural estrogens and androgens), fatty acids (C2-C7), and glutamate (Fahrbach et al, 2008). Moreover, it cannot grow on complex media composed mainly of peptides and carbohydrates.…”

Section: Discussionmentioning

confidence: 99%

“…Second, Sdo. denitrificans has a narrow substrate spectrum and cannot utilize carbohydrates, monoaromatic compounds and long-chain fatty acids (Fahrbach et al, 2008). Third, this bacterium depends on the supply of various vitamins (for example, cobalamin, pyridoxamine and thiamine) for growth (DSMZ Medium 1116).…”

Section: Dcpip-based Spectrophotometric Assaysmentioning

confidence: 99%

“…So far, two anaerobic species (Steroidobacter (Sdo.) denitrificans and Sterolibacterium denitrificans) capable of growing on androgens have been reported (Tarlera and Denner, 2003;Fahrbach et al, 2008). We recently elucidated the anaerobic catabolic pathway (2,3-seco pathway) of testosterone by using Sdo.…”

Section: Introductionmentioning

confidence: 99%

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Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

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Steroid hormones, such as androgens, are common surface-water contaminants. However, literature on the ecophysiological relevance of steroid-degrading organisms in the environment, particularly in anoxic ecosystems, is extremely limited. We previously reported that Steroidobacter denitrificans anaerobically degrades androgens through the 2,3-seco pathway. In this study, the genome of Sdo. denitrificans was completely sequenced. Transcriptomic data revealed gene clusters that were distinctly expressed during anaerobic growth on testosterone. We isolated and characterized the bifunctional 1-testosterone hydratase/dehydrogenase, which is essential for anaerobic degradation of steroid A-ring. Because of apparent substrate preference of this molybdoenzyme, corresponding genes, along with the signature metabolites of the 2,3-seco pathway, were used as biomarkers to investigate androgen biodegradation in the largest sewage treatment plant in Taipei, Taiwan. Androgen metabolite analysis indicated that denitrifying bacteria in anoxic sewage use the 2,3-seco pathway to degrade androgens. Metagenomic analysis and PCR-based functional assays showed androgen degradation in anoxic sewage by Thauera spp. through the action of 1-testosterone hydratase/dehydrogenase. Our integrative 'omics' approach can be used for culture-independent investigations of the microbial degradation of structurally complex compounds where isotopelabeled substrates are not easily available.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Dcpip-based Spectrophotometric Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

et al. 2016

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“…S2-86, Steroidobacter sp. (Fahrbach et al, 2008), Geobacter sp. KB-11 (Lovley et al, 1993), Phenylobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Effect of reclaimed water effluent on bacterial community structure in the Typha angustifolia L. rhizosphere soil of urbanized riverside wetland, China

Huang

Xiong

Liu

et al. 2017

Journal of Environmental Sciences

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In order to evaluate the impact of reclaimed water on the ecology of bacterial communities in the Typha angustifolia L. rhizosphere soil, bacterial community structure was investigated using a combination of terminal restriction fragment length polymorphism and 16S rRNA gene clone library. The results revealed significant spatial variation of bacterial communities along the river from upstream and downstream. For example, a higher relative abundance of γ-Proteobacteria, Firmicutes, Chloroflexi and a lower proportion of β-Proteobacteria and ε-Proteobacteria was detected at the downstream site compared to the upstream site. Additionally, with an increase of the reclaimed water interference intensity, the rhizosphere bacterial community showed a decrease in taxon richness, evenness and diversity. The relative abundance of bacteria closely related to the resistant of heavy-metal was markedly increased, while the bacteria related for carbon/nitrogen/phosphorus/sulfur cycling wasn't strikingly changed. Besides that, the pathogenic bacteria markedly increased in the downstream rhizosphere soil since reclaimed water supplement, while the possible plant growth-promoting rhizobacteria obviously reduced in the downstream sediment.Together these data suggest cause and effect between reclaimed water input into the wetland, shift in bacterial communities through habitat change, and alteration of capacity for biogeochemical cycling of contaminants.

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Sex Control in Tilapias

Baroiller

D'Cotta

2018

Sex Control in Aquaculture

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Steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium

Cited by 185 publications

References 49 publications

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment

Effect of reclaimed water effluent on bacterial community structure in the Typha angustifolia L. rhizosphere soil of urbanized riverside wetland, China

Sex Control in Tilapias

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