Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus

Kumar, Pravesh; Bhat, Irfan Ahmad; Rather, Mohd Ashraf; Gireesh‐Babu, P.; Kumar, Kundan; Purayil, Suresh Babu Padinhate; Sharma, Rupam

doi:10.1016/j.ijbiomac.2017.06.104

Cited by 29 publications

(7 citation statements)

References 44 publications

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“…Likewise, in the present study, we found the instant hormonal surge in A and B treatments which triggered the release of inducing hormones for reproduction in C. magur . However, many researchers have reported that nonadelivery of hormones, drugs, etc, upregulated the reproductive hormones as well as increased the spermatozoa in fishes ( 5 – 7 , 38 ). Similarly, in the present study nanoconjugated nonapeptides and naked nonapeptides were developed and delivered in C. magur.…”

Section: Discussionmentioning

confidence: 99%

Teleost Nonapeptides, Isotocin and Vasotocin Administration Released the Milt by Abdominal Massage in Male Catfish, Clarias magur

et al. 2022

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In the present work the nonapeptides i.e., isotocin and vasotocin alone or in a combination were tested in C. magur to evaluate their effect on stripping by abdominal massage. Also, we used chitosan-carbon nanotube nanocomposites to conjugate the nonapetides isotocin (abbreviated as COOH-SWCNTCSPeP) and isotocin and vasotocin (COOH-SWCNTCSPePs) with the aim of sustaining the effect for a longer duration. The conjugation of nonapeptides with nanocomposites was confirmed by Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), Thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS). Two experiments were conducted to study the effect of naked (without nanoparticles) and conjugated nonapeptides on the milt release by stripping. Both the experiments consisted of eight treatments which included four naked groups two nanoconjugated groups and two controls. Both naked and nonconjugated formulations were successful in stripping the male catfish. The mRNA expression of selected reproductive genes was analysed to decipher the effect of nanopeptides at the molecular level. Nonapeptide treatment either naked or nanoconjugated, resulted in the upregulation of the transcript level of genes. Histological analysis revealed the concentration of spermatozoa was more in peptide injected groups than in the controls. The synergistic effects of nonapeptides and Ovatide had a positive impact on GSI. Thus, the present formulations were successful in stripping the male catfish to obtain the milt with significant reproductive success. Even though the naked groups perform better but the number of males required to fertilize the eggs in nanoconjuagted groups was smaller making it worth using for the delivery of nonapeptides.

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Section: Discussionmentioning

confidence: 99%

Teleost Nonapeptides, Isotocin and Vasotocin Administration Released the Milt by Abdominal Massage in Male Catfish, Clarias magur

et al. 2022

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“…In addition, the majority of nanoparticles raise ROS levels, particularly superoxide, which accelerates the oxidation of molecules like proteins or even DNA (19) and that causes a decline in the number of Leydig cells, which in turn affects the production of testosterone. According to certain research, nanoparticles can affect the expression of the Steroidogenic Acute Regulatory protein (StAR) (20) . This protein is a transmit protein that controls the transfer of cholesterol into the inner mitochondrial membrane and improves the steroid hormones' synthesis.…”

Section: Discussionmentioning

confidence: 99%

Physiological Effect of Green Synthesized Silver Nanoparticles Agnps on Albino Rat Testis

Jasem

Abas

2022

The Egyptian Journal of Hospital Medicine

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Background:The evidence has shown that Silver nanoparticles (AgNPs) are increasingly used in various fields, including medical, food, health care, consumer, and industrial purposes due to their distinctive physical and chemical properties. Despite the advantages of NPs, a number of problems have raised the possibility of toxicity in both people and animals. Objective: The primary aim of the current study is to assess the impact of AgNPs synthesized by green method from plant extract Borago officinalis on reproductive system. Material and methods: AgNPs synthesized by using Borago officinalis flower extracts as reducing agents/stabilizers which be a friendly technique to environment. A total of 40 adult male rats are taken and divided to 4 equal groups: Group 1 treated with distilled water, Group 2 received orally only plant extract (5mg/kg), and Groups 3 and 4 injected through intraperitoneal cavity with AgNPs 30 and 50 mg/kg respectively. We measured oxidative parameters of malondialdehyde (MDA) levels and blood testosterone hormone levels after 35 days. Results: Our results demonstrated that AgNPs rise significantly the MDA value in the animal groups that treated with different concentrations of AgNPs in comparison to the control groups which treated only with Borage extract. Also, a nonsignificant decrease of testosterone levels in animals treated with Borage extract in contrast to control group which have a significant decrease in testosterone which treated with different concentrations of AgNPs. Conclusion:The Borage flower crude extract are nontoxic, where AgNPs (even if it was produced from plant extract) have a negative impact on male reproductive system by alteration the testicular functions especially the testosterone.

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“…In order to investigate the potential mechanisms of RF exposure effect on primary Leydig cells, the expression of genes involved in testosterone synthesis ( Star, Cyp11a1, Hsd-3β ) was examined, and all were found to be altered. Steroidogenic acute regulatory protein (StAR) is able to mediate the production of steroid hormones which is initiated by translocation of cholesterol through the mitochondrial membrane from the outer to the inner 34. Cholesterol was metabolized into pregnenolone by cytochrome P450 Family 11 subfamily A member 1 (CYP11A1), and the hormonally and developmentally regulated expression of CYP11A1 is mainly driven by a variety of trans-acting factors 35.…”

Section: Discussionmentioning

confidence: 99%

<p>CeO<sub>2</sub>NPs relieve radiofrequency radiation, improve testosterone synthesis, and clock gene expression in Leydig cells by enhancing antioxidation</p>

Qin

Shen

Cao

et al. 2019

IJN

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Introduction: The ratio of Ce 3+ /Ce 4+ in their structure confers unique functions on cerium oxide nanoparticles (CeO 2 NPs) containing rare earth elements in scavenging free radicals and protecting against oxidative damage. The potential of CeO 2 NPs to protect testosterone synthesis in primary mouse Leydig cells during exposure to 1,800 MHz radiofrequency (RF) radiation was examined in vitro. Methods: Leydig cells were treated with different concentrations of CeO 2 NPs to identify the optimum concentration for cell proliferation. The cells were pretreated with the optimum dose of CeO 2 NPs for 24 hrs and then exposed to 1,800 MHz RF at a power density of 200.27 µW/cm 2 (specific absorption rate (SAR), 0.116 W/kg) for 1 hr, 2 hrs, or 4 hrs. The medium was used to measure the testosterone concentration. The cells were collected to determine the antioxidant indices (catalase [CAT], malondialdehyde [MDA], and total antioxidant capacity [T-AOC]), and the mRNA expression of the testosterone synthase genes ( Star, Cyp11a1 , and Hsd-3β ) and clock genes ( Clock, Bmal1 , and Rorα ). Results: Our preliminary result showed that 128 μg/mL CeO 2 NPs was the optimum dose for cell proliferation. Cells exposed to RF alone showed reduced levels of testosterone, T-AOC, and CAT activities, increased MDA content, and the downregulated genes expression of Star, Cyp11a1, Hsd-3β, Clock, Bmal1 , and Rorα . Pretreatment of the cells with 128 μg/mL CeO 2 NPs for 24 hrs followed by RF exposure significantly increased testosterone synthesis, upregulated the expression of the testosterone synthase and clock genes, and increased the resistance to oxidative damage in Leydig cells compared with those in cells exposed to RF alone. Conclusion: Exposure to 1,800 MHz RF had adverse effects on testosterone synthesis, antioxidant levels, and clock gene expression in primary Leydig cells. Pretreatment with CeO 2 NPs prevented the adverse effects on testosterone synthesis induced by RF exposure by regulating their antioxidant capacity and clock gene expression in vitro. Further studies of the mechanism underlying the protective function of CeO 2 NPs against RF in the male reproductive system are required.

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Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus

Cited by 29 publications

References 44 publications

Teleost Nonapeptides, Isotocin and Vasotocin Administration Released the Milt by Abdominal Massage in Male Catfish, Clarias magur

Teleost Nonapeptides, Isotocin and Vasotocin Administration Released the Milt by Abdominal Massage in Male Catfish, Clarias magur

Physiological Effect of Green Synthesized Silver Nanoparticles Agnps on Albino Rat Testis

<p>CeO<sub>2</sub>NPs relieve radiofrequency radiation, improve testosterone synthesis, and clock gene expression in Leydig cells by enhancing antioxidation</p>

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