Steroids from <i>Atactodea striata</i> and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines

Abstract

Marine environment is known as a source of potential steroids with multiple biological activities, one of which is an anticancer agent. Atactodea striata are one of the seashells distributed in Indonesia located in the Kei Islands, Southeast Maluku. During the course of our continuing search for biologically active substances from Indonesia seashells, seven steroids have been isolated from the n-hexane fraction of A. striata and they were identified as 7β-hydroxy-sitosterol (1), campesterol (2), β-sitosterol (3), cholesterol (4), 5α,8α-epidioxycholest-6-en-3-β-ol (5), 7-keto-cholesterol (6), and 7α-hydroxy-cholesterol (7). The structure was identified by spectroscopic methods including 2D NMR techniques, FTIR, HRTOFMS, and chemical shift comparison with previously reported spectral data. Compounds 1-7 were evaluated for their cytotoxic effects against MCF-7 breast cancer cells and showed weak or no anticancer activity.

Steroid Compounds of Manihot Esculenta Crantz Var. Sao Pedro Petro (Tuber) and Their Cytotoxic Effects on Melanoma Cancer Cells (B16-F10)

“…The 13 C and DEPT 135° NMR spectrum of compound 1 showed the presence of 6 methyl carbons sp 3 Compound 1 agreed well with data from the literature [27], identified as β-sitosterol and can be seen in Figure 1.…”

“…The 1 H-NMR (CDCl3 500 MHz) spectrum of compound 2 showed the presence of 6 methyl protons sp 3 ), 1 oxygenated methine carbon at δc 71.9 (C-3), 1 olefinic methine carbon at δc 121.8 (C-6), 2 quarternary carbon sp 3 at δc 36.6 (C-10), 42.3 (C-13) and 1 carbon olefinic quarter at δc 140.8 (C-5). Compound 2 agreed well with data from the literature [27], identified as campesterol and this compound can be seen in Figure 2.…”

“…There were 2 sp3 quarternary carbons at δc 36.6 (C-10) and 42.4 (C-13), along with 1 olefinic quartet carbon at δc 140.8 (C-5). Compound 3 agreed well with data from the literature [27], identified as cholesterol and this compound can be seen in Figure 3. A comparison of the results of the 3 compounds is summarized in Table 1.…”