Stevia rebaudiana Bertoni responses to salt stress and chitosan elicitor

Gerami, Mahyar; Majidian, Parastoo; Ghorbanpour, Akram; Alipour, Zeinab

doi:10.1007/s12298-020-00788-0

Cited by 45 publications

(29 citation statements)

References 42 publications

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“…Several studies have found that salinity treatments reduce chlorophyll concentrations in stevia (Janah et al 2021), pepper (Taffouo et al 2017, bean (Alzahrani et al 2019), and peppermint (Fathi et al 2020), which probably happens due to the decreasing of Mg 2+ uptake, an essential mineral element for chlorophyll biosynthesis (Hashem et al 2014). Similar results were found by Gerami et al (2020) in stevia plants subjected to salt stress. However, the use of AMF has proven to have a beneficial effect on chlorophyll contents in this study..…”

Section: Discussionsupporting

confidence: 88%

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Janah

Meddich

Elhasnaoui

et al. 2021

J Soil Sci Plant Nutr

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Salinization of soil is a major problem affecting many plant physiological processes. The use of arbuscular mycorrhizal fungi (AMF) plays a key role in improving the growth and the crops yield. The aim of this study was to evaluate the combined effects of salt stress and AMF on agro-physiological and biochemical responses of Stevia rebaudiana Bertoni. The experiment was carried out in a completely randomized design with ten replicates under the green house conditions. Stevia plants were inoculated with AMF consortium (MC) or with Rhizophagus irregularis (Ri) on the presence (80 mM of NaCl) or the absence (0 mM of NaCl) of salt stress. The results showed that plant height, dry biomass of shoots and roots parts, mycorrhizal frequency and intensity, relative water content, chlorophyll fluorescence, stomatal conductance, chlorophyll a, and total chlorophyll were significantly reduced by 29%, 72%, 36%, 84%, 65%, 24%, 17%, 60%, 28.50%, and 40.9% respectively under salt stress. These changes were associated with a significant increase in antioxidant enzymes (activity of polyphenol oxidase, peroxidase, superoxide dismutase, and catalase), electrolyte leakage, lipid peroxidation, and hydrogen peroxide contents. The application of AMF (MC and Ri) improved growth and physiological parameters through rising the activities of antioxidant enzymes and reduction of the oxidative damage caused by the salt stress. The better results were recorded with MC-inoculated plants. These findings suggested that AMF could be an effective strategy to alleviate the adverse effects of salt stress on Stevia rebaudiana.

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Section: Discussionsupporting

confidence: 88%

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Janah

Meddich

Elhasnaoui

et al. 2021

J Soil Sci Plant Nutr

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“…Oxidative stress is viewed as one of the most significant damaging factors in plant cells exposed to salt stress [ 17 , 18 ]. Plants grown in fields with high levels of salinity are affected by osmotic stress, and therefore, they encounter a great deal of negative effects at the morphological, physiological and biochemical levels [ 19 ]. It is difficult to detach systemic from cellular salinity tolerance mechanisms because of the structural complexity of the entire plant [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Azzam

Al-Taweel

Abdel-Aziz³

et al. 2021

Plants

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Stevia rebaudiana Bertoni is a little bush, which is cultivated on a large scale in many countries for medicinal purposes and used as a natural sweetener in food products. The present work aims to conduct a protocol for stevia propagation in vitro to produce and introduce Stevia rebaudiana plants as a new sweetener crop to Egyptian agriculture. To efficiently maximize its propagation, it is important to study the influence of stress factors on the growth and development of Stevia rebaudiana grown in vitro. Two stevia varieties were investigated (Sugar High A3 and Spanti) against salt stress. Leaves were used as the source of explants for callus initiation, regeneration, multiplication and rooting. Some stress-related traits, i.e., photosynthetic pigments, proline contents, and enzyme activity for peroxidase (POD), polyphenol oxidase (PPO), and malate dehydrogenase (MDH) were studied. Murashig and Skoog (MS) medium was supplemented with four NaCl concentrations: 500, 1000, 2000, and 3000 mgL−1, while a salt-free medium was used as the control. The data revealed that salinity negatively affected all studied characters: the number of surviving calli, regeneration%, shoot length, the number of multiple shoots, number of leaf plantlets−1, number of root plantlets−1, and root length. The data also revealed that Sugar High A3 is more tolerant than Spanti. The total chlorophyll content decreased gradually with increasing NaCl concentration. However, the opposite was true for proline content. Isozyme’s fractionation exhibited high levels of variability among the two varieties. Various biochemical parameters associated with salt tolerance were detected in POD. Namely, POD4, POD6, POD 9 at an Rf of 0.34, 0.57, and 0.91 in the Sugar High A3 variety under high salt concentration conditions, as well as POD 10 at an Rf of 0.98 in both varieties under high salt concentrations. In addition, the overexpression of POD 5 and POD 10 at Rf 0.52 and 0.83 was found in both varieties at high NaCl concentrations. Biochemical parameters associated with salt tolerance were detected in PPO (PPO1, PPO2 and PPO4 at an Rf of 0.38, 0.42 and 0.62 in the Sugar High A3 variety under high salt concentrations) and MDH (MDH 3 at an Rf of 0.40 in both varieties at high salt concentrations). Therefore, these could be considered as important biochemical markers associated with salt tolerance and could be applied in stevia breeding programs (marker-assisted selection). This investigation recommends stevia variety Sugar High A3 to be cultivated under salt conditions.

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“…Based on the previous study, the application of chitosan 0.4 g/L reportedly did not have a significant effect on sunflower (Helianthus annuus L.) [41] and sage (Salvia officinalis L.) [44]. However, the application of chitosan 0.4 g/L and 0.6 g/L can increase the growth parameter in basil (Ocimum basilicum) [45] and stevia (Stevia rebaudiana Bertoni) [46] significantly. That study shows that the application of chitosan 0.4 g/L and 0.6 g/L still has a significant effect on plant growth.…”

Section: Leaves Numbermentioning

confidence: 95%

Chitosan application for maintaining the growth of lettuce (Lactuca sativa) under drought condition

Nurliana

Fachriza

Hemelda

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Drought stress is one of the most common abiotic stress in agriculture. The use of antitranspirant agents to reduce the effect of drought stress on crops has been considered as a potential method. The application of chitosan as an antitranspirant agent has been reported to be effective in several crops. This study was aimed to analyze the effect of chitosan foliar application for maintaining the growth of lettuce under drought conditions. Three concentrations of chitosan (0.2 g/L; 0.4 g/L; 0.6 g/L) were tested in this study. The parameter observed were the height of the lettuce, number of leaves, leaf length and the width, root length, as well as fresh and dry weight. The drought condition without chitosan application reduced the growth of lettuce in all parameters. Chitosan application at 0.2 g/L was able to reduce the effect of drought stress and maintain the growth of lettuce. However, higher chitosan concentration applications (0.4 g/L and 0.6 g/L) were observed to reduce the growth of lettuce. Based on this study, chitosan was reported to reduce the effect of drought stress in lettuce. As chitosan affects the transpiration process by stomatal closure, a higher concentration of chitosan may also affect the photosynthesis process leading to growth inhibition.

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Stevia rebaudiana Bertoni responses to salt stress and chitosan elicitor

Cited by 45 publications

References 42 publications

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Arbuscular Mycorrhizal Fungi Mitigates Salt Stress Toxicity in Stevia rebaudiana Bertoni Through the Modulation of Physiological and Biochemical Responses

Salinity Effects on Gene Expression, Morphological, and Physio-Biochemical Responses of Stevia rebaudiana Bertoni In Vitro

Chitosan application for maintaining the growth of lettuce (Lactuca sativa) under drought condition

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