sti35, a stress-responsive gene in Fusarium spp

Abstract

A stress-induced mRNA was identified in the phytopathogenic fungus Fusarium oxysporum f. sp. cucumerinum. Treatment of the fungus with ethanol resulted in the induction of a major mRNA species encoding a protein of approximate Mr 37,000. A full-length cDNA clone of the induced message was obtained. RNA blot analysis indicated that the mRNA was induced by various other stresses, including treatment with copper(II) chloride and heat (37 degrees C). However, it was not greatly induced by treatment with phaseollinisoflavan, an antifungal isoflavonoid produced by Phaseolus vulgaris (French bean). In contrast, phaseollinisoflavan induced the homologous mRNA in the related bean pathogen Fusarium solani f. sp. phaseoli. A genomic clone of the F. solani f. sp. phaseoli gene was obtained, and both this and the cDNA clone from F. oxysporum f. sp. cucumerinum were sequenced. The latter indicated an open reading frame of 320 codons encoding a 34,556-dalton polypeptide. The corresponding reading frame in F. solani f. sp. phaseoli was 324 codons, 89% identical to the F. oxysporum f. sp. cucumerium sequence, and was interrupted by a short intron. The gene was designated sti35 (stress-inducible mRNA). Although computer homology searches were negative, the cloned gene was observed to cross-hybridize to DNAs of other filamentous fungi, Saccharomyces cerevisiae, and soybean. Thus, sti35 appears to be a common gene among a variety of eucaryotes.

Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance

“…Initially, the main feature of this gene was its homology with the sti35 gene of Fusarium, which is highly induced after stress such as ethanol treatment and heat shock (Choi et al, 1990). Later studies revealed that the THI4 gene is in fact involved in thiamine biosynthesis, in the pathway responsible for production of the thiazole moiety, and its expression is induced by depletion of thiamine (Praekelt et al, 1994).…”

Investigation of stored wheat mycoflora, reporting theFusarium cf.langsethiae in three provinces of Iran during 2007

“…Fungal DNA was extracted using standard protocols, according to Choi et al (1990) and Rezaie et al (2000) with slight modifications. Briefly, the harvested mycelial mass was flash-frozen in liquid nitrogen and ground to a fine powder in a porcelain mortar.…”

Thi1, a thiamine biosynthetic gene inArabidopsis thaliana, complements bacterial defects in DNA repair

“…Screening the database for relat- ed sequences revealed a high homology between the plant gene and three fungal proteins. Two of these are stress-inducible proteins from two species of Fusarium (57.8% identity and 71.2% similarity, excluding the 55 amino acids at the N-terminus of thil, which have no homology to the other gene products, see below) [7]. The other two are genes required for thiamine biosynthesis: the THI4 protein from S. cerevisiae (51.7% identity and 65.4% similarity) [33,34], and the nmt2 gene product from S. pombe (55.1% identity and 67.8% similarity) [26] both of which have been shown to be required for the biosynthesis of the thiazole precursor of thiamine.…”