Stictamycin, an Aromatic Polyketide Antibiotic Isolated from a New Zealand Lichen-Sourced Streptomyces Species

Promoter engineering of natural product biosynthetic gene clusters in actinomycetes: concepts and applications

“…438-3, which produced a new aromatic polyketide named stictamycin ( 1 in Chart 1) in the heterologous host S. albus Del 14. 103 No positive hit rate was reported in this study.…”

Transformation-associated recombination (TAR) cloning and its applications for gene function; genome architecture and evolution; biotechnology and biomedicine

“…More recent examples of TAR application is capture of large BGCs with high G+C content, including 98 kb tylosin (tyl), 128 kb daptomycin (dpt), and 127 kb salinomycin (sal) with their further heterologous expression in Streptomyces coelicolor M1146 to produce tylosins in the resulting recombinant strains [104 ] and 127 kb stictamycin (sal), an aromatic polyketide antibiotic isolated from a New Zealand Lichen-Sourced Streptomyces species with activity against Staphylococcus aureus that is the most pathogenic (it is typically causes skin infections and sometimes pneumonia, endocarditis, and osteomyelitis) [112]. Another group reported a yeast-based platform that exploits TAR cloning for capture, expression, and analysis of a BGC encoding a nonribosomal peptide eponemycin, a novel antibiotic, and TAR capture of TMC-86A that belongs to a family of peptide natural products to clarify the biosynthesis of these important proteasome inhibitors [113].…”