Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

Vitorino, Inês; Klimek, Dominika; Całusińska, Magdalena; Lobo-da-Cunha, Alexandre; Vasconcelos, Vı́tor; Lage, Olga Maria

doi:10.3390/microorganisms10112151

Cited by 6 publications

(6 citation statements)

References 92 publications

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“…The biological material analyzed in this study included twenty-three planctomycetal strains belonging to our culture collection (Laboratory for Microbial Ecophysiology of the University of Porto, Portugal-LEMUP). These strains were previously isolated from different samples collected in marine and estuarine environments in various regions of Portugal (Bondoso, et al , 2015Godinho et al 2021;Lage and Bondoso 2011;Vitorino, et al 2020Vitorino, et al , 2021bVitorino, et al , 2022bVitorino, et al , 2022cVitorino, et al , 2023Vitorino, et al , 2022d. They are all affiliated to the class Planctomycetia within the phylum Planctomycetota.…”

Section: Biological Materialsmentioning

confidence: 99%

“…Coloured branches were used to differentiate each family: green for Lacipirellulaceae, orange for Pirellulaceae and blue for Planctomycetaceae. *data on the antibacterial effects of strain ICT_E10.1 T were taken from previous study (Vitorino, et al 2022b) for the production of bioactive molecules by these strains. Particularly, the extract of R. margarita ICM_H10 T inhibited the growth of all Enterococcus spp.…”

Section: Antimicrobial Activities Of Marine and Estuarine Planctomyce...mentioning

confidence: 99%

“…The vast majority of estimated bacterial diversity is still uncultured in laboratory (Barer and Harwood 1999;Rinke, et al 2013), which heavily restricts what could be screened for biotechnological purposes. Nonetheless, recent cultivation efforts from diverse research groups have allowed to bring into axenic culture many strains (including novel species) from underexplored and evolutionarily deep-branching groups such as the Planctomycetota (Almeida, et al 2022, Gaurav, et al 2021, Kaushik, et al 2020, Kulichevskaya, et al 2020a, Kulichevskaya, et al 2022, Kulichevskaya, et al 2020b, Kumar, et al 2020a, Kumar, et al 2020b, Kumar, et al 2021a, Vitorino, et al 2020, Vitorino et al 2021b, Vitorino, et al 2022b, Vitorino, et al 2022c, Vitorino, et al 2022d. The bacterial phylum Planctomycetes (now renamed Planctomycetota (Oren and Garrity 2021)) belongs to the super phylum Planctomycetota-Chlamydiota-Verrucomicrobiota (PVC) (Rivas-Marín and Devos 2018;Wagner and Horn 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Most Planctomycetota have large genomes with many genes of unknown function and a comparatively high number of genes coding large proteins (Faria, et al 2018;Lage and Bondoso 2014;Wiegand, et al 2018), features that are shared with other well-known producers of bioactive compounds. Genome mining in many strains also demonstrated their rich content of putatively new biosynthetic gene clusters (BGCs) from several structural types (such as nonribosomal peptide synthases (NRPS) polyketide synthases (PKS), ribosomally synthesized and post-translationally modified peptides (RiPPs), lanthipeptides and bacteriocins), which are often linked to the production of bioactive secondary metabolites (Belova, et al 2020;Graca, et al 2016;Jeske, et al 2013;Kallscheuer and Jogler 2021;Vitorino, et al 2022bVitorino, et al , 2022d. Additionally, the capability of some Planctomycetota to produce antimicrobial and cytotoxic molecules was already confirmed through in vitro bioactivity screenings, including the description of the first (and the only one up to date) antimicrobial compounds from planctomycetal origin (Belova, et al 2020;Calisto, et al 2019;Graca, et al 2016;Jeske, et al 2016;Kallscheuer, et al 2020;).…”

Section: Introductionmentioning

confidence: 99%

“…1. Novel recently described species (Godinho et al 2021;Vitorino, et al 2020Vitorino, et al , 2021aVitorino, et al , 2022aVitorino, et al , 2022bVitorino, et al , 2022cVitorino, et al , 2023 were also included in this study. Metabolites from Planctomycetota cultured in oligotrophic medium were extracted with organic solvents and followed by antimicrobial and antifungal assays, in a first stage, against two different types of bacteria (the Gram-negative Escherichia coli and the Gram-positive Staphylococcus aureus) and three different fungi (the yeast Candida albicans and the filamentous fungi Trichophyton rubrum and Aspergillus fumigatus).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

Vitorino,

Pinto,

Martín

et al. 2024

Antonie van Leeuwenhoek

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An appealing strategy for finding novel bioactive molecules in Nature consists in exploring underrepresented and -studied microorganisms. Here, we investigated the antimicrobial and tumoral anti-proliferative bioactivities of twenty-three marine and estuarine bacteria of the fascinating phylum Planctomycetota. This was achieved through extraction of compounds produced by the Planctomycetota cultured in oligotrophic medium followed by an antimicrobial screening against ten relevant human pathogens including Gram-positive and Gram-negative bacteria, and fungi. Cytotoxic effects of the extracts were also evaluated against five tumoral cell lines. Moderate to potent activities were obtained against Enterococcus faecalis, methicillin-sensitive and methicillin-resistant Staphylococcus aureus and vancomycin-sensitive and vancomycin-resistant Enterococcus faecium. Anti-fungal effects were observed against Trichophyton rubrum, Candida albicans and Aspergillus fumigatus. The highest cytotoxic effects were observed against human breast, pancreas and melanoma tumoral cell lines. Novipirellula caenicola and Rhodopirellula spp. strains displayed the widest spectrum of bioactivities while Rubinisphaera margarita ICM_H10T affected all Gram-positive bacteria tested. LC-HRMS analysis of the extracts did not reveal the presence of any known bioactive natural product, suggesting that the observed activities are most likely caused by novel molecules, that need identification. In summary, we expanded the scope of planctomycetal species investigated for bioactivities and demonstrated that various strains are promising sources of novel bioactive compounds, which reenforces the potential biotechnological prospects offered by Planctomycetota.

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Section: Biological Materialsmentioning

confidence: 99%

Section: Antimicrobial Activities Of Marine and Estuarine Planctomyce...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

Vitorino,

Pinto,

Martín

et al. 2024

Antonie van Leeuwenhoek

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Adaptive traits of Planctomycetota bacteria to thrive in macroalgal habitats and establish mutually beneficial relationship with macroalgae

Gao,

Xiao,

Wang

et al. 2024

Limnol Oceanogr Letters

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Bacteria and macroalgae share an inseparable relationship, jointly influencing coastal ecosystems. Within macroalgae habitats, Planctomycetota, a group of bacteria notoriously challenging to cultivate, often dominate. However, the mechanisms facilitating their persistence in this environment remain unclear. Here, we successfully isolated a novel Planctomycetota bacterium, Stieleria sp. HD01, from the surface of kelp. We demonstrated that HD01 possesses a robust ability to metabolize fucoidan, which constitutes half of the kelp‐derived organic carbon and exhibits resistance to attack by most microorganisms. Moreover, HD01 can utilize a broad spectrum of other organics, indicating its metabolic versatility and competitive prowess within algal environments. Additionally, HD01 can secrete antagonistic substances against other bacteria, form biofilms, and employ superoxide dismutase and catalase to resist oxidative stress, further consolidating its ecological fitness. Comparative metagenomics analysis suggested that Planctomycetota may have a mutually beneficial relationship with kelp.

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Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

Godinho,

Klimek,

Jackiewicz

et al. 2023

Antonie van Leeuwenhoek

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A bacterial strain was isolated from a brackish water sample of Tagus river, Alcochete, Portugal and was designated TO1_6T. It forms light pink colonies on M13 medium supplemented with N-acetylglucosamine. Cells are pear-shaped to spherical, form rosettes and divide by budding. Strain TO1_6T presents a mesophilic and neutrophilic profile, with optimum growth at 20 to 25 °C and pH 7.0 to 7.5, and vitamin supplementation is not required to promote its growth. The genome of the novel isolate is 7.77 Mbp in size and has a DNA G + C content of 56.3%. Based on its 16S rRNA gene sequence, this strain is affiliated with the phylum Planctomycetota. Further taxonomic characterization using additional phylogenetic markers, namely rpoB gene sequence (encoding the β-subunit of the DNA-dependent RNA polymerase), as well as Percentage of conserved proteins, average nucleotide identity and average amino acid identity, suggest the affiliation of strain TO1_6T to the genus Stieleria, a recently described taxon in the family Pirellulaceae, order Pirellulales and class Planctomycetia. Based on the genotypic, phylogenetic and physiological characterization, we here describe a new species represented by the type strain TO1_6T (= CECT 30432T, = LMG 32465T), for which the name Stieleria tagensis sp. nov. is proposed.

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Stieleria sedimenti sp. nov., a Novel Member of the Family Pirellulaceae with Antimicrobial Activity Isolated in Portugal from Brackish Sediments

Cited by 6 publications

References 92 publications

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

Uncovering the biotechnological capacity of marine and brackish water Planctomycetota

Adaptive traits of Planctomycetota bacteria to thrive in macroalgal habitats and establish mutually beneficial relationship with macroalgae

Stieleria tagensis sp. nov., a novel member of the phylum Planctomycetota isolated from Tagus River in Portugal

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