Stimulated Chondrogenesis via Chondrocytes Co-culturing

Sayed, Karym El; Zreiqat, Hala

doi:10.4172/2153-0777.s2-003

Cited by 3 publications

(15 citation statements)

References 116 publications

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Section: Introductionmentioning

confidence: 99%

“…Damage to joint cartilage is a challenge in orthopedic medicine because of the limited self-repair potential of this tissue [1]. While cell-based therapies, such as Autologous Chondrocyte doi: 10.7243/2050-1218-5-1 Implantation (ACI), has shown some success for repairing cartilage defects [1], recent efforts have focused on the development of tissue engineered cartilage. Although several different strategies have been proposed to engineer cartilaginous tissue, these approaches rely on a large population of chondrocytes to develop sufficient quantities of tissue in vitro [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

“…Although several different strategies have been proposed to engineer cartilaginous tissue, these approaches rely on a large population of chondrocytes to develop sufficient quantities of tissue in vitro [2][3][4][5]. However, as only a limited number of chondrocytes can be isolated from a cartilage biopsy and these cells tend to dedifferentiate under routine cell expansion [1,6], there is a need for other cell sources in cartilage tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

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Direct and indirect co-culture of bone marrow stem cells and adipose-derived stem cells with chondrocytes in 3D scaffold-free culture

Chiu¹,

Bianco²,

Giardini-Rosa³

et al. 2016

J Regen Med Tissue Eng

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Background: The use of chondrocytes for cartilage tissue engineering is hampered by the limited number of chondrocytes that can be harvested and potential dedifferentiation during cell expansion. While stem cells is a promising approach to create a large population of differentiated cells, multiple growth factors are typically required for differentiation. Alternatively, co-culturing of stem cells with mature chondrocytes can induce differentiation. However, it is not clear which stem cell population and co-culturing method best supports chondrogenesis. While co-culture of stem cells with chondrocytes has been extensively shown to improve chondrogenesis in general, results from previous reports were convoluted by the use of 2D culture or scaffold materials, resulting in discrepancies with the comparison between direct and indirect cocultures. Methods: The purpose of this study was to investigate the extent of chondrogenic differentiation of direct and indirect co-culture of bone marrow stem cells (BMSCs) or adipose-derived stem cells (ASCs) with mature chondrocytes in 3D scaffold-free culture. For direct co-culture, cell pellets were created by centrifugation, consisting of chondrocytes alone or different proportions of stem cells to chondrocytes. For indirect co-culture, cell pellets of chondrocytes or stem cells were created individually and cultured with a separation by a trans-well membrane. Chondrogenic differentiation potential was assessed by quantification of DNA, GAG and collagen contents, as well as collagen I, collagen II and Safranin-O staining. Statistical significance was analyzed using one-way ANOVA with Tukey's post-hoc tests. Results: Direct co-culture of chondrocytes with BMSCs resulted in superior chondrogenesis compared to all other co-culture methods. Cultures with the ratio of 3:1 BMSCs to chondrocytes stained positive for chondrogenic markers and displayed a uniform deposition of cartilaginous extracellular matrix. In addition, the extent of matrix deposition in direct BMSC co-cultures were comparable to growth factor differentiated BMSCs. Conclusions: Thus, BMSCs appear to be superior to ASCs in their differentiation capacity during coculture and a direct co-culture with chondrocytes (3:1 ratio) may be a feasible strategy for cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Direct and indirect co-culture of bone marrow stem cells and adipose-derived stem cells with chondrocytes in 3D scaffold-free culture

Chiu¹,

Bianco²,

Giardini-Rosa³

et al. 2016

J Regen Med Tissue Eng

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“…On the other hand, surface chondrocytes of articular cartilage and mesenchymal stem cells during the cartilage development show cell-to-cell contacts. [137] Co-culture intends to restore direct or indirect interaction between two cell populations in the same functional properties. [137] The mechanisms by which cells interact with each other is not clear but literature reports point towards a combination of cell-to-cell and soluble mediators signalization.…”

Section: Mechanical Loading Stimulationmentioning

confidence: 99%

“…[137] Co-culture intends to restore direct or indirect interaction between two cell populations in the same functional properties. [137] The mechanisms by which cells interact with each other is not clear but literature reports point towards a combination of cell-to-cell and soluble mediators signalization. Cell-to-cell contact formation mechanisms remain unknown but different membrane proteins such as gap junction proteins connexins can affect chondrocyte differentiation.…”

Section: Mechanical Loading Stimulationmentioning

confidence: 99%

Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants

Rochina¹

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3.2.Characterization and validation of ceramic-polymer composite scaffolds 3.3. Development and evaluation of polymer based composite scaffolds: Development of composite scaffolds series 3.3.1. Morphology and microstructure VI 3.3.2. Crystallinity and ceramic content 3.3.3. Mechanical properties 3.3.4. Discussion 3.3.5 Conclusions 3.4. Development and evaluation of polymer based composite scaffolds: PCL/PLLA composite scaffolds degradation study 3.4.1. Morphology and physico-chemical properties 3.4.2 Morphology and microstructure evolution 3.4.3. Weight loss evolution 3.4.4. Mechanical properties 3.4.5. Discussion 3.4.6 Conclusions 3.5. Annex: In vitro and in vivo scaffold evaluation as potential spinal fusion strip 3.5.1. Discussion 3.6. Chapter discussion 4. Macroporous PCL constructs for cartilage tissue engineering: Results and discussion.

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Polymer-Assisted Cartilage and Tendon Repair

Schulze‐Tanzil

2014

Mechanical Engineering Series

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Stimulated Chondrogenesis via Chondrocytes Co-culturing

Cited by 3 publications

References 116 publications

Direct and indirect co-culture of bone marrow stem cells and adipose-derived stem cells with chondrocytes in 3D scaffold-free culture

Direct and indirect co-culture of bone marrow stem cells and adipose-derived stem cells with chondrocytes in 3D scaffold-free culture

Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants

Polymer-Assisted Cartilage and Tendon Repair

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