Stimulated parametric fluorescence microspectroscopy

Label-Free and Low-Invasive Observations of Living Cells with Stimulated Parametric Emission Microscopy

“…The twophoton excited fluorescence (TPEF,) the second-harmonic and third-harmonic generations, and coherent anti-Stokes Raman scattering (CARS) are typical of the nonlinear-optical techniques. I will present a novel technique based on the four wave mixing (FWM) process [1,2] and its applications to living cells. This method is called the stimulated parametric emission (SPE) microscopy and a typical optical setup is shown in Fig.…”