Stimulated production of steroids in Inonotus obliquus by host factors from birch

Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines

“…Extraction yields of the IPTF and OPTF were 0.51 ± 0.04% and 0.78 ± 0.14% on a dry basis, respectively ( Table 1 ). The extraction yield of the OPTF in this study was quite similar to that of an ethyl acetate extract (0.72 ± 0.03%, dry basis) in a previous study [ 6 ]. The previous study also reported that the extraction yield of bark from I. obliquus was higher than that of its inside.…”

“…Total triterpenoids in the OPTF (554.5 ± 13.9 mg ursolic acid equivalent (UAE)/g extract) were significantly higher ( p < 0.05) than those in the IPTF (469.2 ± 10.2 mg UAE/g extract), even when calculated on the basis of the raw materials ( Table 1 ). Wang et al (2014) also reported that total triterpenoids in methanol extracts of birch core and bark of I. obliquus were 2.36 and 2.43 mg/g raw material, respectively [ 6 ].…”

“…Although clinical trials with I. obliquus have been scarce, the safety and efficacy of the mushroom may be evidenced with its traditional use for centuries [ 3 ]. Recently, the extracts from this mushroom have been known to have biological activities such as anti-cancer, anti-inflammatory, and anti-oxidant activities [ 4 , 5 , 6 ]. Previous studies have reported that triterpenoids in I. obliquus have a significant anti-cancer effect on various cancer cells [ 7 , 8 ].…”

Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation

“…Studies have found that the addition of betulin as a stimulating factor to the fermentation medium of Inonotus obliquus can significantly increase the levels of metabolites such as triterpenoids and steroids [17]. Some studies have found that adding birch extract to the culture medium by water extraction can significantly promote the mycelia growth of Inonotus obliquus and the steroid content [18,19,20]. The nutrients of birch extract, which mainly contains fatty acids, polyphenols, triterpenoids, betulin and betulinic acid were studied by HPLC and GC-MS [19,21].…”

“…Some studies have found that adding birch extract to the culture medium by water extraction can significantly promote the mycelia growth of Inonotus obliquus and the steroid content [18,19,20]. The nutrients of birch extract, which mainly contains fatty acids, polyphenols, triterpenoids, betulin and betulinic acid were studied by HPLC and GC-MS [19,21]. Moreover, it can be used as an inducer to induce the production of active polysaccharides, which provides a good direction for future research.…”