Stimulating Effect of Salicylic Acid in the In Vitro and In Vivo Culture of Vanilla (Vanilla planifolia Jacks.)

Abstract

Vanilla planifolia Jacks., is the only commercially cultivated orchid for its compound vanillin. This crop has had serious production limitations due to its susceptibility to Fusarium oxysporum f. sp. vanilla (Fov). Salicylic acid (SA) regulates growth and development processes and induces resistance in plants. Our study evaluated its effect on the growth and development of V. planifolia seedlings grown in vitro and in vivo. A completely randomized design was used with seedlings obtained from nodal segments (2-3 cm long) grown in MS medium with different concentrations of SA (0, 0.25, 5, and 7.5 μmol). An experimental design with four SA concentration treatments (0, 0.25, 0.5, and 1 mmol) and 12 replications was used to evaluate the effects of SA on greenhousegrown plantlets. During four weeks, the number of shoots, length, thickness, number of leaves, number and length roots, chlorophyll and carotene content for both conditions were observed. We found the 7.5 μmol and 1 mmol SA stimulated the growth and development of vanilla plantlets grown both in vivo and in vitro. SA does not affect the content of photosynthetic pigments under any growth conditions.

Complex of Glycyrrhizic and Salicylic Acids: A New Root Length Growing Means for Grapes to Grow from the Apical Meristem

“…Since the complex has not become popular and has not reached the market yet, comparing its effects on grape varieties with other literature is limited to separate components. Salicylic acid has been established to promote in vitro plant growth in Vanilla planifolia Jacks 23 . It was found to support in vitro growth of Hibiscus moscheutos under salt stress 24 .…”