Stimulating effects of two plant growth-promoting bacteria, Enterobacter ludwigii Ez-185-17 and Raoultella terrigena Ez-555-6, on flax culture

Sarron, Elodie; Clément, Nathalie; Pawlicki-Jullian, Nathalie; Gaillard, Isabelle; Boitel-Conti, Michèle

doi:10.1063/1.5033380

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…There are a number of documented species of Enterobacter which have demonstrated multiple plant growth-promoting (PGP) traits. For instance, the P solubilizing and IAA producing Enterobacter ludwigii has been reported to improve the root surface area and the overall growth of flax culture [11]. Whereas in the growth of chickpea and pigeonpea, E. ludwigii particularly increased the iron, zinc, copper, manganese, and calcium uptake of the harvested grains [12].…”

Section: Introductionmentioning

confidence: 99%

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

et al. 2020

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Limited information is available that seed biopriming with plant growth-promoting Enterobacter spp. play a prominent role to enhance vegetative growth of plants. Contrary to Enterobacter cloacae, Enterobacter hormaechei is a less-studied counterpart despite its vast potential in plant growth-promotion mainly through the inorganic phosphorus (P) and potassium (K) solubilization abilities. To this end, 18 locally isolated bacterial pure cultures were screened and three strains showed high P-and K-solubilizing capabilities. Light microscopy, biochemical tests and 16S rRNA gene sequencing revealed that strains 15a1 and 40a were closely related to Enterobacter hormaechei while strain 38 was closely related to Enterobacter cloacae (Accession number: MN294583; MN294585; MN294584). All Enterobacter spp. shared common plant growth-promoting traits, namely nitrogen (N 2) fixation, indole-3-acetic acid production and siderophore production. The strains 38 and 40a were able to produce gibberellic acid, while only strain 38 was able to secrete exopolysaccharide on agar. Under in vitro germination assay of okra (Abelmoschus esculentus) seeds, Enterobacter spp. significantly improved overall germination parameters and vigor index (19.6%) of seedlings. The efficacy of root colonization of Enterobacter spp. on the pre-treated seedling root tips was confirmed using Scanning Electron Microscopy (SEM). The pot experiment of bioprimed seeds of okra seedling showed significant improvement of the plant growth (> 28%) which corresponded to the increase of P and K uptakes (> 89%) as compared to the uninoculated control plants. The leaf surface area and the SPAD chlorophyll index of bioprimed plants were increased by up to 29% and 9% respectively. This report revealed that the under-explored species of P-and K-solubilizing Enterobacter hormaechei sp. with multiple plant beneficial traits presents a great potential sustainable approach for enhancement of soil fertility and P and K uptakes of plants.

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Section: Introductionmentioning

confidence: 99%

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

et al. 2020

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“…strain P-36 was a better-performing auxin/IAA producer when cultivated in VY + Trp (4 mM) than in YES or LB media. In detail, the lowest auxin/IAA concentration was obtained in YES medium (31.4-123.9 mg IAA equ L −1 ), whichever fermentation duration was applied (24,48, and 72 h), while the highest levels occurred when cells were grown on VY medium amended with tryptophan (219.4-459.3 mg IAA equ L −1 ). Data presented in Figure 1 showed that in all tested conditions, while differences were observed in the auxin and biomass yield profiles, the auxin-specific productivity did not significantly change between 48 and 72 h. These data indicated that the auxin production was uncoupled by the biomass production and suggested that, in E. ludwigii strain P36, the production of this metabolite represented an energy-spilling reaction typical of a rate-yield tradeoff phenotype [28].…”

Section: Discussionmentioning

confidence: 93%

“…Several Enterobacter strains have proved so far to exhibit an ability to produce high auxin/IAA levels, which is one of the main direct mechanisms to promote plant growth [20][21][22][23][24][25][26][27]. Bacterial strains, growth medium formulation (e.g., carbon and nitrogen source, concentration of indole auxins precursor), and growing conditions (e.g., agitation speed, temperature, and pH) generally affected indole auxins production, and evidence showed that this is applicable for other strains affiliated with Enterobacteriales (Enterobacter cloacae/ludwigii), as well.…”

Section: Discussionmentioning

confidence: 99%

Production of Indole Auxins by Enterobacter sp. Strain P-36 under Submerged Conditions

Luziatelli

Melini

Bonini³

et al. 2021

Fermentation

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Bioactive compounds produced by plant growth-promoting bacteria through a fermentation process can be valuable for developing innovative second-generation plant biostimulants. The purpose of this study is to investigate the biotechnological potential of Enterobacter on the production of auxin—a hormone with multiple roles in plant growth and development. The experiments were carried in Erlenmeyer flasks and a 2-L fermenter under batch operating mode. The auxin production by Enterobacter sp. strain P-36 can be doubled by replacing casein with vegetable peptone in the culture medium. Cultivation of strain P36 in the benchtop fermenter indicates that by increasing the inoculum size 2-fold, it is possible to reduce the fermentation time from 72 (shake flask cultivation) to 24 h (bioreactor cultivation) and increase the auxin volumetric productivity from 6.4 to 17.2 mg [IAAequ]/L/h. Finally, an efficient storage procedure to preserve the bacterial auxin was developed. It is noteworthy that by sterilizing the clarified fermentation broth by filtration and storing the filtrated samples at +4 °C, the level of auxin remains unchanged for at least three months.

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“…Some Assobio indicator species may present biocontrol roles ( Collimonas fungivorans [ 66 , 67 ]), other less explored/known functions ( Suhomyces canberraensis [ 68 ], Flaviflexus ciconiae [ 69 ], Hygrocybe conica [ 70 ]), and a high number of plant growth promoting species ( Paraburkholderia graminis [ 71 , 72 ], Paraburkholderia phytofirmans [ 73 , 74 ], Sphingobacterium multivorum [ 75 ], Oidiodendron maius [ 76 ], Enterobacter sp. [ 77 , 78 ], Talaromyces islandicus [ 79 ]). These plant-growth promoting microbes are the main group of indicator species found in Assobio terroir.…”

Section: Discussionmentioning

confidence: 99%

“…Enterobacter spp. are able to promote plant growth by aiding nitrogen fixation [ 77 ], IAA production [ 78 ] and by heavy metal removal from soil through siderophore production [ 77 ]. Talaromyces islandicus displays phosphorus solubilization activity improving maise growth and phosphorus uptake [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

A New Perspective for Vineyard Terroir Identity: Looking for Microbial Indicator Species by Long Read Nanopore Sequencing

et al. 2023

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Grapevine is one of the most important fruit crops worldwide, being Portugal one of the top wine producers. It is well established that wine sensory characteristics from a particular region are defined by the physiological responses of the grapevine to its environment and thus, the concept of terroir in viticulture was established. Among all the factors that contribute to terroir definition, soil microorganisms play a major role from nutrient recycling to a drastic influence on plant fitness (growth and protection) and of course wine production. Soil microbiome from four different terroirs in Quinta dos Murças vineyard was analysed through long-read Oxford Nanopore sequencing. We have developed an analytical pipeline that allows the identification of function, ecologies, and indicator species based on long read sequencing data. The Douro vineyard was used as a case study, and we were able to establish microbiome signatures of each terroir.

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Stimulating effects of two plant growth-promoting bacteria, Enterobacter ludwigii Ez-185-17 and Raoultella terrigena Ez-555-6, on flax culture

Cited by 5 publications

References 36 publications

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

Production of Indole Auxins by Enterobacter sp. Strain P-36 under Submerged Conditions

A New Perspective for Vineyard Terroir Identity: Looking for Microbial Indicator Species by Long Read Nanopore Sequencing

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