Stimulating fungal cell wall integrity by exogenous β-glucanase to improve the production of fungal natural products

Discovery of a Fungal P450 with an Unusual Two-Step Mechanism for Constructing a Bicyclo[3.2.2]nonane Skeleton

“…To identify the enzyme responsible for constructing the bicyclo[3.2.2]­nonane structure in the biosynthesis of 1 , potential candidates P450 monooxygenase BTG5 and Baeyer–Villiger monooxygenase BTG16 were selected after analyzing other biosynthetic pathways of heterodimeric bisanthraquinones and related xanthones linked by a single C–C bond. − ,,,, They were then deleted in the Δ CTB1 strain, which will eliminate the influence of the dominant compound cercosporin in the Cercospora sp. JUN001 according to previous studies (Figure S2), ,, of which the intermediates were purified and analyzed. Compared with the metabolic profile of the Δ CTB1 strain at different wavelengths (254 and 330 nm), 11 was obtained from the Δ CTB1 /Δ BTG16 strain (Figure ), suggesting that BTG16 is responsible for the last step of the epoxidation of 11 to afford the final product 1 .…”

“…Although several P450s have the ability to catalyze two-step reactions for the biosynthesis of NPs, 45−48 [37][38][39]41,42,54,55 They were then deleted in the ΔCTB1 strain, which will eliminate the influence of the dominant compound cercosporin in the Cercospora sp. JUN001 according to previous studies (Figure S2), 36,56,57 of which the intermediates were purified and analyzed. Compared with the metabolic profile of the ΔCTB1 strain at different wavelengths (254 and 330 nm), 11 was obtained from the ΔCTB1/ΔBTG16 strain (Figure 2), suggesting that BTG16 is responsible for the last step of the epoxidation of 11 to afford the final product 1.…”