Stimulation of afferent fibres of the guinea‐pig ureter evokes potentials in inferior mesenteric ganglion neurones.

Amann, Rainer; Dray, A.; Hankins, Mark W.

doi:10.1113/jphysiol.1988.sp017220

Cited by 16 publications

(4 citation statements)

References 23 publications

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“…The SP/CGRP fibres observed in the chicken ureteric ganglia most likely represent primary afferent fibres, which are likely to modulate the activity of the ureteric neurones (Sann et al 1992;Hammer et al 1993;Sann et al 1997). A similar modulation by ureteric afferent nerve fibres has been described for sympathetic neurones in the guinea pig inferior mesenteric ganglion (Tsunoo et al 1982;Amann et al 1988).…”

Section: Innervation Of the Neuronessupporting

confidence: 55%

Neuronal subpopulations in autonomic ganglia associated with the chicken ureter: an immunohistochemical study

Sann

1998

Cell and Tissue Research

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The neurochemical coding of neurones located in ganglia of the nerve trunk accompanying the chicken ureter was analysed and quantified using NADPH-diaphorase reactivity and immunohistochemistry against tyrosine hydroxylase (TH), nitric oxide synthase (NOS), calbindin (CAL), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), somatostatin (SOM), substance P (SP) and calcitonin gene-related peptide (CGRP) in untreated or colchicine-treated preparation. Almost all neurones were either positive for TH (38%) or for SOM (60%). Only 4% of the neurones were both TH- and SOM-positive and 3% of the neurones exhibited neither TH nor SOM immunoreactivity. The relative numbers of NPY-, NOS-, CAL- and VIP-positive neurones were 57%, 28%, 14% and 7%, respectively. No SP- or CGRP-positive neurones were observed. All NADPH-diaphorase-positive neurones expressed NOS immunoreactivity. Only in some TH-positive neurones was NPY and/or NOS found. Four major subpopulations were found in the ureteric ganglia. The SOM-positive neurones were subdivided into SOM/NPY/NOS- (28% of all neurones), SOM/NPY- (18%) and SOM/CAL/NPY-positive neurones (14%). A subpopulation of these peptid- ergic neurones also contained VIP. About 35% of the neurones contained TH only. Neurones of all subpopulations (72% of the neurones), except most of the CAL-positive neurones, were encircled by dense plexus of varicose SP/CGRP-positive, presumably sensory nerve fibres. Dense plexus of VIP-positive fibres were observed around 89% of the neurones. The chemical coding of the neuronal subpopulations identified in the ganglia accompanying the chicken ureter resembled that observed in the ganglia of Remak's nerve but was remarkably different from that of the autonomic neurones described in mammalian species.

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Section: Innervation Of the Neuronessupporting

confidence: 55%

Neuronal subpopulations in autonomic ganglia associated with the chicken ureter: an immunohistochemical study

Sann

1998

Cell and Tissue Research

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“…10). Such responses have been described by others, particularly in the IMG where they can be evoked by colonic (Kreulen & Peters 1986), venous (Keef & Kreulen, 1986) or ureteric (Amann, Dray & Hankins, 1988) distension. We found slow responses small and difficult to elicit in coeliac neurones, although readily evoked and larger in five of six IMG neurones we tested (unpublished observations).…”

Section: Slow Responses To Repetitive Nerve Stimulimentioning

confidence: 99%

“…The importance of these small variable responses in coeliac neurones seems questionable because of the unphysiological voltages required to stimulate the axons from which they are derived. However, it has apparently proved relatively easy to evoke such responses in the IMG by natural stimuli (Keef & Kreulen, 1986;Kreulen & Peters, 1986;Amann et al 1988). Clearly an increase in cell resistance during the slow potential would potentiate the effectiveness of subthreshold ESPs in initiating action potentials (see Dun & Ma, 1984).…”

Section: Slow Responses To Repetitive Nerve Stimulimentioning

confidence: 99%

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

McLachlan

Meckler

1989

The Journal of Physiology

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SUMMARY1. Intracellular recordings from sympathetic neurones in the isolated coeliac ganglion of guinea-pigs have been used to define the synaptic input to three subtypes of neurone, classified on the basis of their discharge during maintained depolarizing current as phasic neurones, neurones with prolonged after-hyperpolarizations (LAH), and tonic neurones.2. The three classes of neurone were distributed characteristically in different parts of the ganglion.3. Passive membrane properties differed between the three neurone types. Mean input resistance was highest in phasic neurones and was inversely related to the size of the prolonged calcium-activated potassium conductance in LAH neurones. Mean input time constant was highest in tonic neurones, because of significantly higher cell capacitance.4. Phasic and LAH neurones usually received one suprathreshold ('strong') as well as several subthreshold excitatory synaptic potentials (ESPs) from the ipsilateral splanchnic nerve. In general, the amplitude and number of splanchnic inputs were greater, and the occurrence of two strong inputs more common, in phasic than in LAH neurones. The input to tonic neurones was small and usually subthreshold, even with supramaximal splanchnic stimulation. In a few (mostly tonic) neurones lying close to the midline, small ESPs were evoked by contralateral splanchnic stimulation.5. Antidromic action potentials were evoked in more than half of all neurones by high voltage coeliac nerve stimulation. In addition, multiple small subthreshold ESPs were recorded in virtually all tonic neurones (99 %) on coeliac nerve stimulation. In contrast, coeliac stimulation rarely evoked a few very small ESPs in LAH neurones (9%), but no synaptic response in phasic neurones.6. In about half of the tonic neurones tested (but no phasic or LAH neurones), small ESPs were evoked by stimulation of the intermesenteric nerve.7. Slow depolarization elicited by repetitive activation of splanchnic and coeliac nerve trunks, at voltages supramaximal for the fast cholinergic responses, were * Present address: Department of Physiology and Pharmacology, University of Queensland, St Lucia, Queensland 4067, Australia. E. M. McLACHLAN AND R. L. MECKLERrecorded from about half of both phasic and tonic neurones, but only one of twentyfour LAH neurones. These responses commonly faded during subsequent trials, so that it was difficult to characterize them.8. The data indicate that the three broad groups of coeliac neurone, classified on the basis of their voltage-and calcium-dependent potassium conductances, receive different patterns of synaptic input. The differences may be related to the three major functions of vasoconstriction, motility and mucosal secretion in the small intestine.

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“…The pressure rise results in distension of the proximal ureter and renal pelvis, but it is not clear how this leads to neuronal activity so that pain messages might be transmitted to the brain. Local inflammation caused by the calculus releases inflammatory mediators, such as bradykinin or neurokinin A, which may sensitise afferent neurons via specific receptors [ 2 – 4 ]. However, the clear on-off link between pressure and nerve activity in animal studies and pressure and pain in human studies strongly indicate that the release of inflammatory mediators is not the principal mechanosensory transduction mechanism [ 1 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

ATP release from the human ureter on distension and P2X3 receptor expression on suburothelial sensory nerves

Calvert

Thompson

Burnstock

2008

Purinergic Signalling

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It is not clear how the increase in intraluminal pressure behind an obstructing ureteric calculus causes an increase in action potential frequency in ureteric sensory nerves so the pain messages are transmitted to the brain. It has been proposed that ureteric distension causes urothelial release of ATP, which activates purinoceptors on suburothelial nociceptive sensory nerves. The purpose of this study was to determine whether distension of the human ureter results in the release of ATP and whether the nociceptive P2 receptor, P2X 3 , is expressed on suburothelial sensory nerves in the human ureter. Human ureter segments were perfused with Krebs solution and intermittently distended to a range of pressures. Samples of perfusate were collected throughout and the ATP concentration ([ATP]) was determined using a luciferin-luciferase assay. Sections of ureter were stained using antibodies against P2X 3 and capsaicin receptors (TRPV1).[ATP] rose to more than 10 times baseline levels after distension beyond a threshold of 25-30 cmH 2 O. Immunofluorescence studies on consecutive frozen sections showed that suburothelial nerves stained positively for P2X 3 and capsaicin receptors, with no staining in controls. These findings are consistent with the hypothesis that purinergic signalling is involved in human ureteric mechanosensory transduction, leading to nociception.

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Stimulation of afferent fibres of the guinea‐pig ureter evokes potentials in inferior mesenteric ganglion neurones.

Cited by 16 publications

References 23 publications

Neuronal subpopulations in autonomic ganglia associated with the chicken ureter: an immunohistochemical study

Neuronal subpopulations in autonomic ganglia associated with the chicken ureter: an immunohistochemical study

Characteristics of synaptic input to three classes of sympathetic neurone in the coeliac ganglion of the guinea‐pig.

ATP release from the human ureter on distension and P2X3 receptor expression on suburothelial sensory nerves

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