Stimulation of butyrate production through the metabolic interaction among lactic acid bacteria, <i>Lactobacillus acidophilus</i>, and lactic acid‐utilizing bacteria, <i>Megasphaera elsdenii</i>, in porcine cecal digesta

Tsukahara, Takamitsu; Hashizume, Kenta; Koyama, Hideki; Ushida, Kazunari

doi:10.1111/j.1740-0929.2006.00372.x

Cited by 46 publications

(45 citation statements)

References 39 publications

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“…Butyrate represents the most preferential energy source of colonocytes [69], resulting in the stimulation of epithelial cell proliferation and mucus secretion [70]. Therefore, the supplementation of B. subtilis and B. licheniformis may contribute to an improved gut health of pigs.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Kaewtapee

Burbach

Tomforde

et al. 2017

J Animal Sci Biotechnol

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Background Bacillus spp. seem to be an alternative to antimicrobial growth promoters for improving animals’ health and performance. However, there is little information on the effect of Bacillus spp. in combination with different dietary crude protein (CP) levels on the ileal digestibility and microbiota composition. Therefore, the objective of this study was to determine the effect of Bacillus spp. supplementation to low- (LP) and high-protein diets (HP) on ileal CP and amino acid (AA) digestibility and intestinal microbiota composition.MethodsEight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a row-column design with 8 pigs and 3 periods of 16 d each. The assay diets were based on wheat-barley-soybean meal with two protein levels: LP (14% CP, as-fed) and HP diet (18% CP, as-fed). The LP and HP diets were supplemented with or without Bacillus spp. at a level of 0.04% (as-fed). The apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA was determined. Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR. Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS.ResultsThe supplementation with Bacillus spp. did not affect both AID and SID of CP and AA in growing pigs. Moreover, there was no difference in AID of CP and AA between HP and LP diets, but SID of cystine, glutamic acid, glycine, and proline was lower (P < 0.05) in pigs fed the HP diets. The HP diets increased abundance of Bifidobacterium spp. and Lactobacillus spp., (P < 0.05) and by amplicon sequencing the latter was identified as predominant genus in microbiota from HP with Bacillus spp., whereas dietary supplementation of Bacillus spp. increased (P < 0.05) abundance of Roseburia spp..ConclusionsThe HP diet increased abundance of Lactobacillus spp. and Bifidobacterium spp.. The supplementation of Bacillus spp. resulted in a higher abundance of healthy gut associated bacteria without affecting ileal CP and AA digestibility, whereas LP diet may reduce the flow of undigested protein to the large intestine of pigs.

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Section: Discussionmentioning

confidence: 99%

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Kaewtapee

Burbach

Tomforde

et al. 2017

J Animal Sci Biotechnol

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“…Each individual was designated as an experimental block so that the time lags of dissection and sample preparation would not bias the results [22]. When significant differences were detected, the post-hoc Tukey-Kramer multiple comparison test was used.…”

Section: Methodsmentioning

confidence: 99%

Oral Administration of EC-12 Increases the Baseline Gene Expression of Antiviral Cytokine Genes, IFN-γ and TNF-α, in Splenocytes and Mesenteric Lymph Node Cells of Weaning Piglets

Tsuruta

Inoüe

Tsushima

et al. 2013

Bioscience of Microbiota, Food and Health

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Weaning piglets are continuously exposed to various viruses. The antiviral effects of lactic acid bacteria (LAB) have been confirmed mainly in humans and mice, while few studies have been conducted in livestock. In this study, we evaluated the effect of oral administration of Enterococcus faecalis strain EC-12 (EC-12) on the gene expressions of antiviral cytokines in weaning piglets. Piglets were allocated to the EC-12-administered group (E group) and the no-treatment control group (C group). The small intestinal tissue, the mesenteric lymph node (MLN) cells and the splenocytes were collected from the piglets. The tissue and cells were co-cultured with a live vaccine of porcine reproductive respiratory syndrome virus, porcine epidemic diarrhea virus or EC-12. After the incubation, the gene expressions of IFN-γ and TNF-α in the tissue and cells were evaluated. The gene expressions of IFN-γ in the MLN cells and TNF-α in the splenocytes were significantly higher in the E group than in the C group. However, the increase in the gene expression of antiviral cytokines was observed independently of the antigen treatments. The results of the present study suggest that oral administration of EC-12 did not increase the response of immune cells to specific viral antigens but increased the baseline gene expression of antiviral cytokines.

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“…2003). A lactate‐utilizing butyrate producer, Megasphaera elsdenii , increased butyrate production in combination with lactic acid bacteria (Tsukahara et al. 2006).…”

Section: Introductionmentioning

confidence: 99%

Oral administration of Lactobacillus plantarum Lq80 and Megasphaera elsdenii iNP‐001 induces efficient recovery from mucosal atrophy in the small and the large intestines of weaning piglets

Yoshida

Tsukahara

Ushida

2009

Animal Science Journal

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Weaning causes atrophy of intestinal mucosa and a drop of IgA protection in piglets which increases vulnerability to pathogenic infections. Probiotic lactobacilli may support recovery from such weaning stresses. Butyrate-produce bacteria may support the growth of colonic mucosa. Megasphaera elsdenii, a lactate-utilizing butyrate producer, may help butyrate production particularly when combined with lactobacilli. Weaned piglets (Experiment 1: 20 days old, Experiment 2: 28 days old) were orally dosed once a day with either (L) 10(10) (cell/dose) L. plantarum Lq80, or (LM) 10(10) (cell/dose) Lq80 with 10(9) (cell/dose) M. elsdenii iNP-001. Lq80 was contained in capsules resistant to gastric digestion. M. elsdenii was contained in capsules resistant to gastric and intestinal digestion. An untreated control (C) was also prepared. After 2 weeks of administration, L. plantarum enhanced the recovery from the villous atrophy in both experiments. The rectal and colonic IgA tended to be higher in L and LM than in C in Experiment 1. Colonic butyrate was higher in LM than in the others in Experiment 1. The thickness of the colonic mucosa was greater in LM than in the others in Experiment 1. In early weaned piglets, the effects of L. plantarum and M. elsdenii were clear.

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Stimulation of butyrate production through the metabolic interaction among lactic acid bacteria, Lactobacillus acidophilus, and lactic acid‐utilizing bacteria, Megasphaera elsdenii, in porcine cecal digesta

Cited by 46 publications

References 39 publications

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Oral Administration of EC-12 Increases the Baseline Gene Expression of Antiviral Cytokine Genes, IFN-γ and TNF-α, in Splenocytes and Mesenteric Lymph Node Cells of Weaning Piglets

Oral administration of Lactobacillus plantarum Lq80 and Megasphaera elsdenii iNP‐001 induces efficient recovery from mucosal atrophy in the small and the large intestines of weaning piglets

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