Stimulation of Canine Gastric Mucus Secretion With Intraarterial Acetylcholine Chloride

Zalewsky, Charlotte A.; Moody, Frank G.; Allen, M.J.; Davis, E.K.

doi:10.1016/s0016-5085(83)80073-0

Cited by 18 publications

(4 citation statements)

References 17 publications

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“…Characterization of the in vitro model. The ACh analog carbachol was employed in initial experiments because cholinomimetics have been found to stimulate secretion from secretory cells (6,37,46). Carbachol (3 µM) reproducibly evoked large dilations of Brunner's gland acinar lumen (see Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The secretory action of cholinergic agonists has been observed in a wide variety of secretory cells, including columnar and goblet cells (6,37) and gastric surface mucus cells (46), and a number of cholinergic neural pathways mediating this action have been implicated. Within the gastrointestinal tract, the cholinergic regulation of secretory responses can involve intrinsic and/or extrinsic pathways (6).…”

Section: G481mentioning

confidence: 99%

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A novel in vitro model of Brunner's gland secretion in the guinea pig duodenum

Moore¹,

Morris

Vanner³

2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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A novel in vitro model that combined functional and morphological techniques was employed to directly examine pathways regulating Brunner's gland secretion in isolation from epithelium. In vitro submucosal preparations were dissected from guinea pig duodenum. A videomicroscopy technique was used to measure changes in luminal diameter of glandular acini as an index of activation of secretion. Carbachol elicited concentration-dependent dilations of the lumen (EC(50) = 2 microM) by activating muscarinic receptors on acinar cells. Ultrastructural and histological analyses demonstrated that dilation was accompanied by single and compound exocytosis of mucin-containing granules and the accumulation of mucoid material within the lumen. Inflammatory mediators (histamine, PGE(1), PGE(2)) and intestinal hormones (CCK, gastrin, vasoactive intestinal polypeptide, secretin) also stimulated glandular secretion, whereas activation of submucosal secretomotor neurons by 5-hydroxytryptamine did not. This study directly demonstrates that multiple hormonal, inflammatory, and neurocrine agents activate Brunner's glands, whereas many have dissimilar effects on the epithelium. This suggests that Brunner's glands are regulated by pathways that act both in parallel to and in isolation from those controlling epithelial secretion.

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Section: Resultsmentioning

confidence: 99%

Section: G481mentioning

confidence: 99%

A novel in vitro model of Brunner's gland secretion in the guinea pig duodenum

Moore¹,

Morris

Vanner³

2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Acetylcholine induced a higher level of mucin secretion when applied to the serosa compared to luminal application(83), depleting goblet cells of mucin within 60 mins compared to untreated controls(82). Acetylcholine may stimulate innervated mucus cells or increase hydrostatic pressure to induce mucin release(84). When chloride (Cl − ) secretion was inhibited after acetylcholine treatment, liquid secretion was blocked up to 70%, and there was an accumulation of mucin in excised porcine bronchial gland ducts(85).…”

Section: Strengthening the Mucus Barriermentioning

confidence: 99%

Engineering the Mucus Barrier

Carlson

Lock

2018

Annu. Rev. Biomed. Eng.

114

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Mucus selectively controls the transport of molecules, particulate matter, and microorganisms to the underlying epithelial layer. It may be desirable to weaken the mucus barrier to enable effective delivery of drug carriers. Alternatively, the mucus barrier can be strengthened to prevent epithelial interaction with pathogenic microbes or other exogenous materials. The dynamic mucus layer can undergo changes in structure (e.g., pore size) and/or composition (e.g., protein concentrations, mucin glycosylation) in response to stimuli that occur naturally or are purposely administered, thus altering its barrier function. This review outlines mechanisms by which mucus provides a selective barrier and methods to engineer the mucus layer from the perspective of strengthening or weakening its barrier properties. In addition, we discuss strategic design of drug carriers and dosing formulation properties for efficient delivery across the mucus barrier.

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“…One method for assessing mucus secretion is to measure changes in the thickness of the mucous gel layer resulting from exposure of the stomach to secretary agents MS 7726 A. C. KEATES AND P. J. HANSON in vivo (Bickel & Kauffman, 1981; McQueen, Hutton, Allen & Garner, 1983). Such work showed that changes in mucous gel thickness could be dissociated from changes in soluble mucus release, and that prostaglandins, cholinergic stimulation and secretin could increase the thickness of the mucous gel layer (McQueen et al 1983; Zalewsky, Moody, Allen & Davis, 1983; McQueen, Allen & Garner, 1984;Allen, Carroll & Hirst, 1986). Recently, the effects of prostaglandins on mucus secretion were investigated by using pieces of rabbit gastric mucosa maintained in vitro by organ culture, but no data were provided for the actions of other potential secretagogues (Seidler, Knafla, Kownatzki & Sewing, 1988).…”

Section: Introductionmentioning

confidence: 99%

Regulation of mucus secretion by cells isolated from the rat gastric mucosa.

Keates

Hanson

1990

The Journal of Physiology

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SUMMARY1. A suspension of cells, containing about 30 % mucous cells, was isolated from the rat fundic mucosa, and was pre-incubated with D-[6-311]glucosamine. 3H-labelled material subsequently released into the medium was separated by Fast Protein Liquid Chromatography on a Superose 6 column.2. A sharp peak of labelled high molecular weight material eluted from the column close to the void volume. This material was identified as mucous glycoprotein by its similar chromatographic behaviour to partially purified rat gastric mucous glycoprotein, by its resistance to complete degradation by papain and by its behaviour on treatment with dithiothreitol. On a caesium chloride density gradient the labelled material was virtually all located between densities of 1t35 and 1-53 g/ml, with the main peak at 1-40 g/ml.3. A broad peak of lower molecular weight material was also eluted from the column. The release of this unidentified material did not seem to be closely associated with the release of mucous glycoprotein from the cells.4. Release of mucous glycoprotein was stimulated by secretin (half-maximally effective concentration 2-a nM, 84% stimulation above basal release at 100 nM), and by isoprenaline (half-maximally effective concentration 34 nm, 33 % stimulation at 1 tM). Carbachol (0-5 mM) produced a significant (18-29%) stimulation of mucus secretion, but gastrin (100 nM), histamine (0-5 mM) and epidermal growth factor (200 nM) were without effect.5. The preparation should prove useful in the identification of the agents which regulate gastric mucus secretion.

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Stimulation of Canine Gastric Mucus Secretion With Intraarterial Acetylcholine Chloride

Cited by 18 publications

References 17 publications

A novel in vitro model of Brunner's gland secretion in the guinea pig duodenum

A novel in vitro model of Brunner's gland secretion in the guinea pig duodenum

Engineering the Mucus Barrier

Regulation of mucus secretion by cells isolated from the rat gastric mucosa.

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