Stimulation of cellular RNA synthesis in mouse-kidney cell cultures infected with SV40 virus

May, Pierre; E, May; Borde, J

doi:10.1016/0014-4827(76)90175-0

Cited by 38 publications

(24 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using an in vitro transcription system, we have reconstituted this process using recombinant large T antigen and purified Pol I transcription factors. In vitro, recombinant large T antigen stimulated RNA Pol I transcription up to fivefold, which is similar to what has been observed in vivo (this work; May et al 1976;Pockl and Wintersburger 1980). These results suggest that the in vitro system faithfully reproduces the transcriptional activation function of large T antigen and therefore is an important tool for the identification and biochemical analysis of the cellular proteins that mediate this process.…”

Section: Discussionsupporting

confidence: 87%

“…Identical results were obtained using recombinant large T antigen pufflied by either conventional or affinity chromatography. These results established that large T antigen, when added to HeLa nuclear extract, stimulated RNA Pol I transcription to levels comparable to the ones that have been reported in vivo (this work; May et al 1976).…”

Section: T Antigen Can Stimulate Rrna Synthesis In a Defined Rna Pol supporting

confidence: 89%

“…Early studies showed that infection of mammalian cells with SV40 induced rRNA synthesis (May et al 1976;Pockl and Wintersburger 1980). Further analysis indicated that the product of the viral gene A, the large tumor antigen (large T antigen), was directly involved in this process (Ide et al 1977;Soprano et al 1981;Learned et al 1983).…”

mentioning

confidence: 99%

See 2 more Smart Citations

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

Zhai¹,

Tuan²,

Comai³

1997

Genes Dev.

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 87%

Section: T Antigen Can Stimulate Rrna Synthesis In a Defined Rna Pol supporting

confidence: 89%

See 1 more Smart Citation

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

Zhai¹,

Tuan²,

Comai³

1997

Genes Dev.

View full text Add to dashboard Cite

“…For example, the synthesis of rRNA is sensitive to a variety of physiological signals such as response to nutrient starvation, regulation by cell cycle, the state of cell proliferation, and invasion by viral infection (6,9,17,24,26,27,31). In addition, an interesting and unexpected property of rRNA transcription is the inability of the transcriptional machinery of one species to initiate transcription from the promoter of divergent species.…”

mentioning

confidence: 99%

Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis.

Haltiner¹,

Smale²,

Tjian³

1986

Mol. Cell. Biol.

126

View full text Add to dashboard Cite

A cell-free RNA polymerase I transcription system was used to evaluate the transcription efficiency of 21 linker scanning mutations that span the human rRNA gene promoter. Our analysis revealed the presence of two major control elements, designated the core and upstream elements, that affect the level of transcription initiation. The core element extends from -45 to +18 relative to the RNA start site, and transcription is severely affected (up to 100-fold) by linker scanning mutations in this region. Linker scanning and deletion mutations in the upstream element, located between nucleotides -156 and -107, cause a three-to fivefold reduction in transcription. Under certain reaction conditions, such as the presence of a high ratio of protein to template or supplementation of the reaction with partially purified protein fractions, sequences upstream of the core element can have an even greater effect (20-to 50-fold) on RNA polymerase I transcription. Primer extension analysis showed that RNA synthesized from all of these mutant templates is initiated at the correct in vivo start site. To examine the functional relationship between the core and the upstream region, mutant promoters were constructed that alter the orientation, distance, or multiplicity of these control elements relative to each other. The upstream control element appears to function in only one orientation, and its position relative to the core is constrained within a fairly narrow region. Moreover, multiple core elements in close proximity to each other have an inhibitory effect on transcription.The cellular machinery responsible for RNA synthesis is designed to respond to a variety of specific signals so that genes can be transcribed and expressed in a controlled fashion. For example, the synthesis of rRNA is sensitive to a variety of physiological signals such as response to nutrient starvation, regulation by cell cycle, the state of cell proliferation, and invasion by viral infection (6,9,17,24,26,27,31). In addition, an interesting and unexpected property of rRNA transcription is the inability of the transcriptional machinery of one species to initiate transcription from the promoter of divergent species. Thus, whereas both sets of rRNA genes can be transcribed in an interspecific rodent cell hybrid, the genes from only one species are expressed in rodent-human cell hybrids (22,32). This species specificity is mimicked in vitro in that extracts from one species are capable of transcribing only those rDNA templates that are derived from the same or closely related species (5,12,14). The limited homology in the DNA sequence surrounding the transcription start site of mammalian rRNA genes suggests that species-specific promoter recognition is likely to involve distinctive rRNA promoter elements.Studies with fractionated enzyme components derived from mammalian cell extracts have allowed the identification of at least two distinct activities that are necesary to reconstitute accurate and efficient initiation of transcription from the human ribosomal...

show abstract

“…Sci. U.S.A., in press), and induces host cell DNA and RNA syntheses (6,15,27,28,39,42,45,55,56,64). T antigen also possesses a "helper function" that enables adenovirus to grow more efficiently in simian cells (13,21,31,49).…”

mentioning

confidence: 99%

Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.

Manos¹,

Gluzman²

1984

Mol. Cell. Biol.

View full text Add to dashboard Cite

The large T antigen of simian virus 40 (SV40) is a multifunctional protein that is essential in both the virus lytic cycle and the oncogenic transformation of cells by SV40. To investigate the role of the numerous biochemical and physiological activities of T antigen in the lytic and transformation processes, we have studied DNA replication-deficient, transformation-competent large T-antigen mutants. Here we describe the genetic and biochemical analyses of two such mutants, C2/SV40 and C11/SV40. The mutants were isolated by rescuing the integrated SV40 DNA from C2 and Cll cells (CV-1 cell lines transformed with UVirradiated SV40). The mutant viral early regions were cloned into the plasmid vector pK1 to generate pC2 and pC1l. The mutations that are responsible for the deficiency in viral DNA replication were localized by marker rescue. Subsequent DNA sequencing revealed point mutations that predict amino acid substitutions in the carboxyl third of the protein in both mutants. The pC2 mutation predicts the change of Lys --Arg at amino acid 516. pC1l has two mutations, one predicting a change of Pro -* Ser at residue 522, and another predicting a Pro --Arg change at amino acid 549. The two Cll mutations were separated from each other to form two distinct viral genomes in pCllA and pC11B. pC2, pCll, pC11A, and pCllB are able to transform both primary and established rodent cell cultures. The Cll and C1lA T antigens are defective in ATPase activity, suggesting that wild-type levels of ATPase activity are not necessary for the oncogenic transformation of cells by T antigen.The multifunctional large T antigen of simian virus 40 (SV40) is essential in both the lytic cycle of the virus and the oncogenic transformation of cells by SV40. T antigen, encoded by the SV40 A gene, is expressed early in the lytic pathway and functions in numerous processes throughout the infectious cycle. It is required for the initiation of viral DNA replication (59), autoregulates early viral RNA synthesis (1, 51, 52, 61), stimulates late viral transcription (30;

show abstract

Stimulation of cellular RNA synthesis in mouse-kidney cell cultures infected with SV40 virus

Cited by 38 publications

References 3 publications

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

Two distinct promoter elements in the human rRNA gene identified by linker scanning mutagenesis.

Simian virus 40 large T-antigen point mutants that are defective in viral DNA replication but competent in oncogenic transformation.

Contact Info

Product

Resources

About