Stimulation of diesel degradation and biosurfactant production by aminoglycosides in a novel oil-degrading bacterium Pseudomonas luteola PRO23

Abstract

Bioremediation is promising technology for dealing with oil hydrocarbons contamination. In this research growth kinetics and oil biodegradation efficiency of Pseudomonas luteola PRO23, isolated from crude oil-contaminated soil samples, were investigated under different concentrations (5, 10 and 20 g/L) of light and heavy crude oil. More efficient biodegradation and more rapid adaptation and cell growth were obtained in conditions with light oil. The 5 to 10 g/L upgrade of light oil concentration stimulated the microbial growth and the biodegradation efficiency. Further upgrade of light oil concentration and the upgrade of heavy oil concentration both inhibited the microbial growth, as well as biodegradation process. Aminoglycosides stimulated biosurfactant production in P. luteola in the range of sub-inhibitory concentrations (0.3125, 0.625 ?g/mL). Aminoglycosides also induced biofilm formation. The production of biosurfactants was the most intense during lag phase and continues until stationary phase. Aminoglycosides also induced changes in P. luteola growth kinetics. In the presence of aminoglycosides this strain degraded 82% of diesel for 96 h. These results indicated that Pseudomonas luteola PRO23 potentially can be used in bioremediation of crude oil-contaminated environments and that aminoglycosides could stimulate this process. [Projekat Ministarstva nauke Republike Srbije, br. TR31080]

Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles

“…Pseudomonas luteola has previously been described [ 33 ]. Pseudomonas aeruginosa strongly degrades hydrocarbons [ 34 ] as does Pseudomonas stutzeri [ 35 ]. Pantoea calida has not previously been found to degrade hydrocarbons.…”

Study of bacterial interactions in reconstituted hydrocarbon-degrading bacterial consortia from a local collection, for the bioremediation of weathered oily-soils

“…The biodegradability of classes of TPH organics is variable. The most susceptible to biodegradation are n -alkanes and isoprenoid aliphatic alkanes [ 24 ]. Monoaromatic steroids, diasteranes, 25-norhopanes, C 21 -C 22 steranes, tricyclic terpanes, oleanane, gammacerane and diahopanes are the most resistant [ 25 ].…”

Bioreactors for remediation of hydrocarbons in rivers and lagoons of San Luis Potosí

“…after four days of the culture, using the Pseudomonas luteola bacteria stimulated by 338 aminoglycosides in a SFB (Atanasković {\it et al}., 2016); the degradation was more than 339 that obtained in our research in a similar timeframe of five days, where the initial 340 concentration was reduced to 4,020 $\pm$ 170 mg L$^{-1}$. because in the riser zone, where air-flow circulation in ALB begins, air bubbles have a larger diameter than air bubbles of the downcomer zone where only arrive small bubble size, causing a difference of flow between the riser zone and the downcomer zone, generating a lower volumetric mass transfer coefficient (kLa) in the downcomer zone with respect to the riser zone (Zheng et al, 2018).…”