Stimulation of glycogen degradation by prostaglandin E2 in primary cultured rat hepatocytes

α-Lipoic Acid Prevents the Induction of iNOS Gene Expression Through Destabilization of Its mRNA in Proinflammatory Cytokine-Stimulated Hepatocytes

“…Hepatocytes were isolated from male Wistar strain rats (200-220 g; Charles River, Tokyo, Japan) by collagenase (Wako Pure Chemicals, Osaka, Japan) perfusion [17,18]. Isolated hepatocytes were suspended in culture medium at 6 9 10 5 cells/ml, seeded into 35-mm plastic dishes (2 ml/ dish; Falcon Plastic, Oxnard, CA, USA) and cultured at 37°C in a CO 2 incubator under a humidified atmosphere of 5% CO 2 in air.…”

Up-regulation of IL-1 receptor through PI3K/Akt is essential for the induction of iNOS gene expression in hepatocytes

“…Hepatocytes were isolated from male Wistar strain rats (200-250 g) by collagenase perfusion and were cultured [27]. After 7 h, the medium was replaced by Williams' medium E (WE medium) without serum and hormones, and cells were cultured overnight and used for experiments.…”

Hypoxia and Heat Inhibit Inducible Nitric Oxide Synthase Gene Expression by Different Mechanisms in Rat Hepatocytes

“…Hepatocytes were isolated from male Wistar strain rats (200-250 g) by collagenase perfusion as described previously. 34 All animal experiments were approved by the Animal Care Committee of Kansai Medical University. The isolated hepatocytes were suspended in culture medium at 5.5 to 6.0 ϫ 10 5 cells/mL, seeded onto plastic dishes (2 mL/dish, 35 ϫ 10 mm; 9 cm 2 , Falcon Plastic, Oxnard, CA) and then cultured as monolayers in a CO 2 incubator (under a humidified atmosphere of 5% CO 2 in air) at 37°C.…”