Stimulation of human osteoblast cells (MG63) proliferation using decanoic acid and isopropyl amine fractions of <i>Wattakaka volubilis</i> leaves

Venugopal, Elakkiya; Ramadoss, Govindarajan; Krishnan, Kannan; Eranezhath, Sujith; Bhattacharyya, Amitava; Selvakumar, R.

doi:10.1111/jphp.12801

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…(MG63) cells(Venugopal et al, 2017). The current study further validated that fatty acids can regulate the bone remodeling in FHN(Bani Hassan et al, 2019;Kishikawa et al, 2019).…”

supporting

confidence: 72%

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

Zhu

Wang

Luo

et al. 2023

Preprint

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Introduction Femur head necrosis (FHN) is a challengeable clinical disease with unclear underlying mechanism and a low rate of early diagnosis. Objectives To elucidate altered metabolism pathways in FHN and identify putative biomarkers for the detection of FHN. Methods we recruited 26 patients with femur head necrosis and 22 patients with femur neck fracture in this study. Cancellous bone tissues from the femoral heads were collected after the surgery and were analyzed using an untargeted metabolomics approach on the basis of gas chromatography-mass spectrometry (GC-MS). Results The resulting data were analyzed via uni- and multivariate statistical approaches, and we identified 38 metabolites distinctly changed in FHN group. Among them, 33 metabolites were upregulated and 5 were downregulated in FHN. The pathway analysis results showed that linoleic acid metabolism were the most relevant to FHN pathology. On the basis of metabolites network, L-lysine, L-glutamine and L-serine were deemed as the junctions of the whole metabolites. At last, we figured out that 9,12-octadecadienoic acid, inosine, L-proline and octadecanoic acid can be considered as the potential biomarkers of FHN. Conclusion Our study provided a new insight into the pathogenesis of FHN and identified 4 biomarkers in FHN. Linoleic acid metabolism could be considered as the core in FHN and 9,12-Octadecadienoic acid could be considered as the diagnostic marker.

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“…(MG63) cells(Venugopal et al, 2017). The current study further validated that fatty acids can regulate the bone remodeling in FHN(Bani Hassan et al, 2019;Kishikawa et al, 2019).…”

supporting

confidence: 72%

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

Zhu

Wang

Luo

et al. 2023

Preprint

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“…Furthermore, capric acid inhibits the micellar growth in vitro of Candida albicans due to a resulting 0.20 of its fractionated inhibitory concentration index for 70% growth inhibition, which is considered significant [90] . It potentially impacts the stimulation of the human osteoblast (MG63) by using decanoic acid fractions from Wattakaka volubilis leaves [91] . Finally, caproic acid can be utilized to increase gene delivery without affecting biocompatibility [92] .…”

Section: Virgin Coconut Oil As An Adjunctive Treatment For Various Ailments 431 Virgin Coconut Oil As An Adjunctive Treatment For Variousmentioning

confidence: 99%

“…[ 90,91] Hexanoic acid Caproic acid 6:0 Caproic acid can be utilized to increase gene delivery and enhance the delivery of protein.…”

Section: Systematic Name Common Name Carbon Skeleton Established Therapeutic Uses Referencesmentioning

confidence: 99%

The potential use of virgin coconut oil as an adjunctive treatment for COVID-19: A review

Dacasin¹,

Diagono²,

So³

et al. 2021

J Pharmacogn Phytochem

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“…The details of phytochemicals used in this study such as extraction, characterization and identification can be seen in our previous report [19]. Phytochemical incorporated gelatin nanofibers were prepared using the electrospinning process.…”

Section: Preparation Of Nanofibrous Scaffoldmentioning

confidence: 99%

“…One of the most important medicinal plants widely used in an orthopaedic application is Wattakaka volubilis. In our previous study, we reported that the active phytochemical compounds present in W. volubilis leaves can potentially be used to increase cellular proliferation and ECM production in bone and cartilage cells under optimized dosage [19].…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of phytochemical loaded electrospun gelatin nanofibers for application in bone and cartilage tissue engineering

et al. 2018

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Wattakaka volubilis, a medicinal plant, is known to exhibit various potential health benefits and has traditionally been used in Ayurveda for various medicinal applications. In the present study, phytochemicals hexadecanoic acid, octadecanoic acid and N,N-Diisopropyl(2,2,3,3,3-pentafluoropropyl)amine isolated from W. volubilis leaf extract were co-electrospun with gelatin nanofibers for meniscus and osteoblast cell attachment and proliferation. The electrospun nanofibers were characterized using suitable techniques such as a scanning electron microscope and Fourier transform infrared spectroscopy. The mechanical property of electrospun gelatin nanofibers and phytochemicals incorporated gelatin nanofibers were tensile tested. Both the control and phytochemical loaded nanofiber exhibited a similar stress–strain trend. The average diameter of the control and phytocompound loaded gelatin nanofiber was found to be 300 ± 5.5 nm and 483 ± 12 nm, respectively. The rate of biodegradation of the control and phytochemical loaded nanofiber was analyzed in a simulated body fluid. The cell attachment and proliferation were monitored using a fluorescence microscope after appropriate staining. The cell viability, DNA content, extracellular secretion confirmed that the phytocompound loaded gelatin nanofibers were non-toxic and enhanced the meniscus and osteoblast cell growth and proliferation. This phytocompound loaded gelatin matrix may be used as a potential scaffold for cartilage and bone tissue engineering applications.

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Stimulation of human osteoblast cells (MG63) proliferation using decanoic acid and isopropyl amine fractions of Wattakaka volubilis leaves

Cited by 7 publications

References 19 publications

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

Comparative metabolomic analysis of human cancellous bone reveals the critical role of linoleic acid metabolism in femur head necrosis based on GC-MS

The potential use of virgin coconut oil as an adjunctive treatment for COVID-19: A review

In vitro evaluation of phytochemical loaded electrospun gelatin nanofibers for application in bone and cartilage tissue engineering

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