Stimulation of human peripheral blood lymphocytes by bioactive peptides derived from bovine milk proteins

Kayser, Holger; Meisel, Hans

doi:10.1016/0014-5793(96)00207-4

Cited by 175 publications

(127 citation statements)

References 12 publications

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“…While cell proliferation is promoted by peptides from fish (Ravallec Ple et al 2001;Ravallec Ple et al 2000), soybean (Franek et al 2000), wheat (Franek et al 2000), and milk (Kayser and Meisel 1996), cell growth inhibition by peptides derived from soybean (Kops et al 1997) and milk (Ganjam et al 1997) has been described. Peptides derived from wheat have been found to inhibit (Giovannini et al 2000;Silano and De Vincenzi 1999) and promote (Rivabene et al 1999;Rocca et al 1983) cell death.…”

Section: Discussionmentioning

confidence: 99%

Chickpea protein hydrolysate as a substitute for serum in cell culture

et al. 2008

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The growth of mammalian cells in vitro requires the use of rich culture media that are prepared by combining serum with specific nutrient formulations. Serum, the most expensive component of culture media, provides a complex mixture of growth factors and nutrients. Protein hydrolysates that can support in vitro cell growth and eliminate or reduce the need to use serum have been obtained from different sources. Here we describe the use of two food grade proteases to produce a chickpea protein hydrolysate that has been added to cell culture medium in order to determine whether it can be used as a substitute for serum. Medium containing the hydrolysate has been tested using two human cells lines: the monocytic THP-1 cell line which grows in suspension, and the epithelial Caco-2 cell line which grows as a monolayer. The chickpea protein hydrolysate was a good substitute for serum in the first case, but did not allow growth of Caco-2 cells. Supplementation of culture media with this inexpensive and safe hydrolysate would greatly reduce the cost of cell culture.

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Section: Discussionmentioning

confidence: 99%

Chickpea protein hydrolysate as a substitute for serum in cell culture

et al. 2008

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“…β-Casomorphin-7 derived from β-casein was used as a possible immunostimulating substrate, since it is a ligand of opioid receptors that have also been found on the surface of human T-lymphocytes (33)(34)(35).…”

Section: Immunomodulatory Peptidesmentioning

confidence: 99%

“…Dipeptide (Tyr-Gly), which corresponds to a partial sequence in the primary structure of bovine κ-casein (residues 38-39), was used for immunotherapy of human immunodefi ciency virus infection (35,38).…”

Section: Immunomodulatory Peptidesmentioning

confidence: 99%

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Summer

Formaggioni

Franceschi

et al. 2017

Food Technol. Biotechnol.

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SummaryItalian hard cooked types of cheese, like Parmigiano Reggiano and Grana Padano, are characterised by positive nutritional qualities. In fact, they contain substances that have particular biological activities, and therefore they can be fully considered, according to the defi nition given by the European Unit, as 'functional' foods. This short review concisely describes these components and the benefi cial eff ects related to their activities. The description of the biologically active components has been organised in the following paragraphs: protein and peptides, fat and lipids, carbohydrates and prebiotics, probiotic bacteria, vitamins, mineral salts, and components of dairy products active in disease prevention. In particular, several known bioactive peptides were found in Parmigiano Reggiano cheese samples: for example, phosphopeptides, which are known for their mineral-binding capacity and vehiculation activity, peptides with immunomodulatory activity, and angiotensin-converting enzyme-inhibitory peptides with anti-hypertensive eff ects. Among lipids, the role of conjugated linoleic acid and other fatt y acids present in these cheese types was taken into consideration. The presence of oligosaccharides with prebiotic properties and probiotic bacteria was also described. Finally, particular emphasis was given to highly available calcium and its impact on bone health.

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“…Proliferation changes were measured as described in [17] using a colorimetric enzyme immunoassay (Boehringer Mannheim, Germany, kit 1647229) based on incorporation of 5-bromo-2'-deoxyuridine (BrdU) during DNA synthesis within 17 h (Caco-2) or 24 h (PBL, HL-60). Results were read out on an ELISA spectrophotometer (Anthos HT-2) at 450 nm (reference wavelength: 620 nm).…”

Section: Measurement Of Proliferation and Apoptosismentioning

confidence: 99%

Apoptosis induced by modified ribonucleosides in human cell culture systems

et al. 1998

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The in vitro modulation of apoptosis and cell proliferation by modified in comparison with non-modified ribonucleosides was investigated for the first time using peripheral blood lymphoeytes, HL-60 cells and Caco-2 cells as human cell culture models. Modulating effects of several ribonucleosides were found in the range of 10-~-10 s mol/i. The following ribonuclensides induced significant apoptosis of HL-60 cells: adenosine, N6-dimethyladenosine, N~-(2-isopentenyl)-adenosine, N2-dimethylguanosine. A significant apoptotic effect on PBL was found with N6-dimethyladenosine and N°-(2 -isopentenyl)-adenosine. N6-Dimethyladenosine, N6-(2-isopentenyl)-adenosine and guanosine had a pronounced inhibitory effect on Caco-2 cell apoptosis. Regarding the known function of ribonucleosides as pathobiochemical marker molecules for cancer, the possibility of a selective apoptotic effect against malignant cells is discussed.

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Stimulation of human peripheral blood lymphocytes by bioactive peptides derived from bovine milk proteins

Cited by 175 publications

References 12 publications

Chickpea protein hydrolysate as a substitute for serum in cell culture

Chickpea protein hydrolysate as a substitute for serum in cell culture

Cheese as Functional Food: The Example of Parmigiano Reggiano and Grana Padano

Apoptosis induced by modified ribonucleosides in human cell culture systems

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