Stimulation of in Vitro Granulocyte‐Macrophage Colony Formation by Mouse Heart Conditioned Medium

Byrne, Peter V.; Heit, W; Kubanek, B

doi:10.1111/j.1365-2141.1978.tb03657.x

Cited by 14 publications

(3 citation statements)

References 13 publications

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“…CFU-C were assayed according to the method of Bradley & Metcalf (1966) as modified by Iscove (1972). MNC 105 were cultured in 1 ml a-medium containing 0.8% methylcellulose, 20% horse serum and 20% mouse heartconditioned medium (Byrne et at., 1978).…”

Section: Methodsmentioning

confidence: 99%

Effects of cisplatin on different haemopoietic progenitor cells in mice

Nowrousian¹,

Schmidt²

1982

Br J Cancer

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Summary.-The effects of Cisplatin on marrow haemopoietic progenitor cells, WBC and RBC were measured and compared in F1 (CBA x C57BL) female mice. Dose/ survival curves of Cisplatin for CFU-S, CFU-C and BFU-E were found to be simply exponential, indicating that the effect of the drug has no cell-cycle dependency. BFU-E also appeared significantly (P <0001) more sensitive to Cisplatin than CFU-S and CFU-C. After a single dose of 12 mg/kg of Cisplatin, WBC, MNC and CFU-E were seen to be markedly less reduced and to recover much earlier than CFU-C, and particularly BFU-E and CFU-S. Results suggest that the drug is more toxic for earlier haemopoietic progenitor cells than for the more mature cells, and that the latter are not reliable parameters for complete haemopoietic recovery in mice after treatment with this agent. In the animals treated, there was also a subsequent significant decrease of the RBC count, accompanied by a marked increase of the marrow CFU-E concentration. Possible underlying mechanisms (e.g. alterations of RBC after exposure to Cisplatin) were discussed.

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Section: Methodsmentioning

confidence: 99%

Effects of cisplatin on different haemopoietic progenitor cells in mice

Nowrousian¹,

Schmidt²

1982

Br J Cancer

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“…This indicates that this MGI-1M differed antigenically from MGI-1G and MGI-2. The functional specificities of MGI-1M and MGI-1G may be associated with a preferential activity on specific normal myeloid progenitors in the heterogeneous progenitor population in the bone marrow (Haskill et al, 1970;Byrne et al, 1978).…”

Section: Discussionmentioning

confidence: 99%

Separation of different molecular forms of macrophage‐ and granulocyte‐inducing proteins for normal and leukemic myeloid cells

Lotem

Lipton

Sachs

1980

Intl Journal of Cancer

105

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It is shown that serum of mice treated with endotoxin (ES) contains three separable and functionally distinct forms of macrophage- and granulocyte-inducing (MGI) proteins. One form (MGI-1M) induced the formation of macrophage colonies from normal bone-marrow cells and showed on gel filtration an apparent molecular weight of 300,000; a second form (MGI-1G) induced the formation of granulocyte colonies from normal bone-marrow cells and had an apparent molecular weight of 45-100,000; and the third form (MGI-2) induced the normal differentiation of MGI+D+ myeloid leukemic cells to macrophages and granulocytes and had an apparent molecular weight of 28,000. Studies on the time course of the decrease of these three activities in ES have indicated that MGI-2 was more readily inactivated in vivo than MGI-1M and MGI-1G. The MGI-1M in ES isolated after gel filtration was completely neutralized by an antiserum to MGI-1 from mouse L-cells, whereas the isolated MGI-1G and MGI-2 were not affected by this antiserum. Gel filtration under dissociating conditions (6 M guanidinium chloride) resulted in a reduction of the apparent molecular weights of MGI-1M from 300,000 to 42,000, and of MGI-1G from 45-100,000 to 28,000, while it produced no change in the 28,000 apparent molecular weight of MGI-2. Similar studies with conditioned medium produced in vitro from mouse lung and peritoneal macrophages showed that in these conditioned media, MGI-1 (both G and M) in the native form had an apparent molecular weight of 41,000 and MGI-2 of 24,000, and that both MGI-1 and 2 had an apparent molecular weight of 24,000 under dissociating conditions. The results indicate that MGI-1 exists in serum in vivo and in these conditioned media as aggregated proteins, whereas MGI-2 does not, and that macrophages and lung tissue are not the only source of the MGI proteins found in ES. It is suggested that all three forms of MGI activity are derived from one precursor protein; that only the MGI-2 form assayed on leukemic cells should be used for treatment based on the induction of normal cell differentiation in myeloid leukemia; and that MGI-2 may serve as a survey mechanism for inducing differentiation in myeloid leukemic cells that have lost their responsiveness to the MGI-1 molecules that control the viability, proliferation and differentiation of normal myeloblasts.

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“…Cells at a concentration of 0.2 x 1Q6Mml1 were cultured in a-medium containing 5% v/v newborn calf serum and 15% donor horse serum (Gibco). Mouse heart conditioned medium was used as a source of colony stimulating factor (Byrne et al, 1978).…”

Section: Human Cfugm Assaymentioning

confidence: 99%

Effect of adriamycin on CFUGM at plasma concentrations found following therapeutic infusions

Bailey‐Wood¹,

Dallimore²,

Whittaker³

1984

Br J Cancer

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Summary The effect of adriamycin on human and mouse CFUGM was examined at concentrations and times suggested by plasma clearance data derived from the results of a number of published studies. Our results suggest that the high concentrations of drug present in the plasma for short periods of time following infusion are only weakly cytotoxic towards the CFUGM when incubated for similar times. In contrast, there was a considerably greater cytotoxic effect when the drug was examined at low concentrations for periods similar to those described for the terminal phase of adriamycin clearance. The principal metabolite, adriamycinol, was poorly cytotoxic.

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Stimulation of in Vitro Granulocyte‐Macrophage Colony Formation by Mouse Heart Conditioned Medium

Cited by 14 publications

References 13 publications

Effects of cisplatin on different haemopoietic progenitor cells in mice

Effects of cisplatin on different haemopoietic progenitor cells in mice

Separation of different molecular forms of macrophage‐ and granulocyte‐inducing proteins for normal and leukemic myeloid cells

Effect of adriamycin on CFUGM at plasma concentrations found following therapeutic infusions

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