Stimulation of local antibody production: parenteral or mucosal vaccination?

Jp, Bouvet; Decroix, Nipa; Pamonsinlapatham, Perayot

doi:10.1016/s1471-4906(02)02186-5

Cited by 57 publications

(46 citation statements)

References 51 publications

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“…In contrast to other vaccines having to rely on mucosal delivery to access the natural inductive pathway (29,35,39), B 4 T can induce high mucosal immune responses by parenteral administration. Several different, nonexclusive mechanisms have been proposed to explain the production of secretory antibodies after parenteral administration of the antigen, including direct diffusion of soluble or phagocytosed antigens to mucosa-associated lymphoid tissue or activation of antigen-presenting cells at draining lymph nodes, which then migrate to mucosa-associated lymphoid tissue (12). In any event, parenterally administered antigens capable of inducing strong mucosal immunity, such as our B 4 T dendrimer or a few other immunogens so far described (28,37,56), appear to be good candidates for future mucosal vaccines.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide

Cubillos

Torre

Jakab

et al. 2008

J Virol

102

101

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The successful use of a dendrimeric peptide to protect pigs against challenge with foot-and-mouth disease virus (FMDV), which causes the most devastating animal disease worldwide, is described. Animals were immunized intramuscularly with a peptide containing one copy of a FMDV T-cell epitope and branching out into four copies of a B-cell epitope. The four immunized pigs did not develop significant clinical signs upon FMDV challenge, neither systemic nor mucosal FMDV replication, nor was its transmission to contact control pigs observed. The dendrimeric construction specifically induced high titers of FMDV-neutralizing antibodies and activated FMDV-specific T cells. Interestingly, a potent anti-FMDV immunoglobulin A response (local and systemic) was observed, despite the parenteral administration of the peptide. On the other hand, peptideimmunized animals showed no antibodies specific of FMDV infection, which qualifies the peptide as a potential marker vaccine. Overall, the dendrimeric peptide used elicited an immune response comparable to that found for control FMDV-infected pigs that correlated with a solid protection against FMDV challenge. Dendrimeric designs of this type may hold substantial promise for peptide subunit vaccine development.

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Section: Discussionmentioning

confidence: 99%

Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide

Cubillos

Torre

Jakab

et al. 2008

J Virol

102

101

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“…This suggests that while parasite-specific serum IgG may have a role in natural resistance and parenterally induced protective immunity (14,39), such responses are not absolutely required for the expression of protection in the oral vaccine model. The mechanism of protection in this model has not been characterized yet, although stimulation of secretory IgA responses that neutralize the activity of parasite proteins seems likely (10,34) and locally secreted IgG (11) may also play a role. Oral immunization may also have primed cellular responses, leading to accelerated mucosal mastocytosis upon challenge infection; such responses are known to occur after secondary challenge in hamsters (5,30).…”

Section: Discussionmentioning

confidence: 99%

Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms

et al. 2004

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Hookworms remain major agents of global morbidity, and vaccination against these bloodfeeding parasites may be an attractive complement to conventional control methods. Here we describe the cloning of Ancylostoma ceylanicum excretory-secretory protein 2 (AceES-2), a novel immunoreactive protein produced by adult worms. Native AceES-2 was purified from excretory-secretory (ES) products by reverse-phase high-pressure liquid chromatography, subjected to amino-terminal sequencing, and cloned from adult worm RNA by using reverse transcription-PCR. The translated AceES-2 cDNA predicts that the mature protein consists of 102 amino acids and has a molecular mass of 11.66 kDa. Western immunoblot and enzyme-linked immunosorbent assay analyses demonstrated that recombinant AceES-2 (rAceES-2) reacted strongly with antibodies from A. ceylanicum-infected hamsters. Immunization of hamsters with native ES products adsorbed to alum induced antibodies that recognized rAceES-2, while rAceES-2-alum vaccination resulted in antibodies that reacted with a single protein band in ES products that closely approximated the size predicted for the native molecule. Infected hamsters that were passively immunized with hyperimmune rabbit anti-rAceES-2 serum exhibited more rapid and complete recovery from anemia than controls that received normal serum. Oral immunization with rAceES-2 was associated with significantly reduced anemia upon challenge, an outcome similar to the outcome observed in hamsters that were orally vaccinated with soluble hookworm extract (the latter animals were also resistant to weight loss). These data suggest that AceES-2 plays an important role in the hostparasite interaction and that vaccination against this protein may represent a useful strategy for controlling hookworm anemia.

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“…Small controlled doses of Ag can be precisely delivered by needle injection into the skin or muscle. Mucosally delivered vaccines, despite their being easy to administer, however, require much higher doses of Ag to effectively stimulate adaptive immunity and cannot be accurately controlled (12,35). Unfortunately, orally delivered vaccines do not induce good adaptive immune responses unless toxic adjuvants such as cholera toxin are coadministered (36,37).…”

Section: Cd11bmentioning

confidence: 99%

TLR-Induced Local Metabolism of Vitamin D3 Plays an Important Role in the Diversification of Adaptive Immune Responses

Enioutina

Bareyan

Daynes

2009

The Journal of Immunology

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The addition of monophosphoryl lipid A, a minimally toxic derivative of LPS, to nonmucosally administered vaccines induced both systemic and mucosal immune responses to coadministered Ags. This was dependent on an up-regulated expression of 1α-hydroxylase (CYP27B1, 1αOHase), the enzyme that converts 25-hydroxycholecalciferol, a circulating inactive metabolite of vitamin D3, into 1,25(OH)2D3 (calcitriol). In response to locally produced calcitriol, myeloid dendritic cells (DCs) migrated from cutaneous vaccination sites into multiple secondary lymphoid organs, including classical inductive sites of mucosal immunity, where they effectively stimulated B and T cell immune responses. The endogenous production of calcitriol by monophosphoryl lipid A-stimulated DCs appeared to be Toll-IL-1R domain-containing adapter-inducing IFN-β-dependent, mediated through a type 1 IFN-induced expression of 1αOHase. Responsiveness to calcitriol was essential to promote the trafficking of mobilized DCs to nondraining lymphoid organs. Collectively, these studies help to expand our understanding of the physiologically important roles played by locally metabolized vitamin D3 in the initiation and diversification of adaptive immune responses. The influences of locally produced calcitriol on the migration of activated DCs from sites of vaccination/infection into both draining and nondraining lymphoid organs create a condition whereby Ag-responsive B and T cells residing in multiple lymphoid organs are able to simultaneously engage in the induction of adaptive immune responses to peripherally administered Ags as if they were responding to an infection of peripheral or mucosal tissues they were designed to protect.

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Stimulation of local antibody production: parenteral or mucosal vaccination?

Cited by 57 publications

References 51 publications

Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide

Enhanced Mucosal Immunoglobulin A Response and Solid Protection against Foot-and-Mouth Disease Virus Challenge Induced by a Novel Dendrimeric Peptide

Purification and Molecular Cloning of and Immunization with Ancylostoma ceylanicum Excretory-Secretory Protein 2, an Immunoreactive Protein Produced by Adult Hookworms

TLR-Induced Local Metabolism of Vitamin D3 Plays an Important Role in the Diversification of Adaptive Immune Responses

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