Stimulation of lymphocytes from subacute sclerosing panencephalitis patients by defined measles virus antigens

Ilonen, Jorma; Reunanen, Mauri; Herva, Elja; Ziola, Barry; Salmi, A.

doi:10.1016/0008-8749(80)90253-1

Cited by 20 publications

(9 citation statements)

References 34 publications

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“…These two studies and a study employing a model protein (15) show that T cells showing cross-reactivity for internal virus proteins can, through cognate effects, influence immune responses during a subsequent virus infection not only to the protein carrying the cross-reactive epitopes (the viral internal antigen), but also to other B and T cell non-cross-reactive epitopes (the viral surface antigens). That a similar mechanism might operate for Paramyxoviridae viruses was further suggested by the finding (B. Ziola, unpublished results) that immunizing cyclophosphamidetreated mice with trypsin-derivatized MV ribonucleoprotein (11,40) mixed in dimethyl dioctadecyl ammonium bromide gave rise to T cells that recognized RSV and MPV, but not vesicular stomatitis virus and influenza A virus, in a delayed-type hypersensitivity assay.…”

Section: Discussionmentioning

confidence: 88%

Prior Infection by Respiratory Syncytial Virus or Parainfluenza Viruses Augments Virus-Specific IgG Responses Induced by the Measles/Mumps/Rubella Vaccine

Ziola

Karvonen²,

Stewart³

1994

Viral Immunology

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We found previously that immunizing cyclophosphamide-treated mice with one Paramyxoviridae virus mixed with dimethyl dioctadecyl ammonium bromide induces T cells which apparently also recognize other Paramyxoviridae viruses. This finding and the fact that respiratory syncytial virus (RSV) and parainfluenza viruses (PIVs) infect children early in life led us to ask if prior RSV or PIV infections influence the antibody response to measles and mumps vaccine viruses. Detection of virus-specific IgG in serum specimens collected randomly or at defined times after measles/mumps/rubella (MMR) vaccination was done with solid-phase enzyme immunoassays. The antibody-binding data obtained were converted to serum antibody titers by an immunoassay curve-fitting computer program. Prior infection by RSV and PIVs correlated with an augmented IgG response not only to measles and mumps virus, but also to rubella virus. Furthermore, the augmentation was greater for responders below the median response. These data show that common early childhood viral infections can influence immunity induced by the MMR vaccine.

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Section: Discussionmentioning

confidence: 88%

Prior Infection by Respiratory Syncytial Virus or Parainfluenza Viruses Augments Virus-Specific IgG Responses Induced by the Measles/Mumps/Rubella Vaccine

Ziola

Karvonen²,

Stewart³

1994

Viral Immunology

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“…12). We have earlier demonstrated the T cell specificity of this measles lymphoblast transformation assay in a patient with subacute sclerosing panencephalitis with an abnormally strong response to different measles antigens [15],…”

Section: Discussionmentioning

confidence: 92%

Lymphocyte Blast Transformation Responses in Measles Infection

et al. 1980

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Lymphocyte blast transformation responses were studied in eight patients with natural measles infection. A specific response to purified measles virus antigen and a weaker response to crude measles virus antigen were found after infection. The response to purified measles antigen appeared 1-3 weeks after the beginning of the rash, when the great number of proliferating mononuclear cells found in the peripheral blood during the rash was declining. In the first weeks after onset of the rash also leucocyte and lymphocyte numbers were decreased, and specific responses to purified tuberculin (PPD) and to rubella and mumps virus antigens were suppressed. In mitogen stimulation tests there was no significant suppression of phytohaemagglutinin and concanavalin responses in this small series, but the response to pokeweed mitogen was decreased. The responses to antigens other than measles virus antigens recovered in parallel with the increase of the measles-specific response.

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“…Virus was purified from culture supernatants by the potassium tartrate gradient method described by Anttonen et al (1980) and nucleocapsid from the infected cell pellet essentially according to the CsC1 gradient method of Ilonen et al (1979) but with the addition of a third gradient composed of 1.2 ml aliquots of 20~, 25%, 30%, 35~ and 40~ CsCl in phosphate-buffered saline (PBS). Following centrifugation at 4 °C in an SW27.1 rotor at 24000 r.p.m, for 16 h, the nucleocapsid bands were removed, diluted with PBS and pelleted in an SW27.…”

Section: Cells and Virusmentioning

confidence: 99%

Immunoassays for Measles Virus Nucleocapsid Antigen: Effect of Antigen-Antibody Complexes

Salmi

Lund

1984

Journal of General Virology

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SUMMARYAn inhibition radioimmunoassay and a two-site immunoradiometric (four-layer) assay for the quantification of measles virus nucleocapsid antigen were established and their sensitivities compared. Both assays exhibited threshold sensitivities of from 1 to 10 ng nucleocapsid antigen per ml. Concentrations of reagents used in the assays were shown to be an important factor determining the sensitivities of the assays. Purified mumps or parainfluenza 2 virus nucleocapsids did not compete with measles virus nucleocapsids in the inhibition assay but some degree of cross-reactivity with canine distemper virus nucleocapsid was observed. Pretreatment of virus-infected cells with detergent had a significant effect on the amount of antigen detectable by the assays. SDS greatly decreased the reactivity of measles virus nucleocapsid but Triton X-100 and, to a larger degree, sodium deoxycholate released antigen from the cells in quantities greater than that detected when no detergent pretreatment was employed. Complexing of antibodies to measles virus nucleocapsids in vitro decreaseddramatically the threshold sensitivity of the nucleocapsid assays. The antigen-antibody complexes could be disrupted and the components separated but the conditions employed destroyed 90% of the antigenic reactivity of the nucleocapsid and also had a deleterious effect on the antibody. Nucleocapsid antigen was readily detected in brains from hamsters with acute measles encephalitis. The assays, however, were not sensitive enough for routine detection of antigen in brains from hamsters with chronic measles encephalitis.

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Stimulation of lymphocytes from subacute sclerosing panencephalitis patients by defined measles virus antigens

Cited by 20 publications

References 34 publications

Prior Infection by Respiratory Syncytial Virus or Parainfluenza Viruses Augments Virus-Specific IgG Responses Induced by the Measles/Mumps/Rubella Vaccine

Prior Infection by Respiratory Syncytial Virus or Parainfluenza Viruses Augments Virus-Specific IgG Responses Induced by the Measles/Mumps/Rubella Vaccine

Lymphocyte Blast Transformation Responses in Measles Infection

Immunoassays for Measles Virus Nucleocapsid Antigen: Effect of Antigen-Antibody Complexes

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