Stimulation of Macrophage Oxygen Free Radical Production and Lymphocyte Blastogenic Response by Immunization with Porins

Tabaraie, Bahman; Sharma, Bal Krishan; Sharma, Praveen; Sehgal, Rakesh; Ganguly, Nirmal Kumar

doi:10.1111/j.1348-0421.1994.tb01823.x

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…They have been investigated as possible adhesion molecules and virulence factors in host cell recognition. Association of porins from Salmonella with host cells triggers a variety of biological events that include induction of cell-mediated Arai 1989b, 1992) and humoral (Tabaraie et al 1994) immune responses and induction of cytokine gene expression (Galdiero et al 1998). More recently, Salmonella porins have been shown to activate cell-signaling pathways in human macrophages (Galdiero et al 2002(Galdiero et al , 2003.…”

Section: Introductionmentioning

confidence: 99%

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OmpD but not OmpC is involved in adherence ofSalmonella entericaserovar Typhimurium to human cells

Hara-Kaonga¹,

Pistole²

2004

Can. J. Microbiol.

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Conflicting reports exist regarding the role of porins OmpC and OmpD in infections due to Salmonella enterica serovar Typhimurium. This study investigated the role of these porins in bacterial adherence to human macrophages and intestinal epithelial cells. ompC and ompD mutant strains were created by transposon mutagenesis using P22-mediated transduction of Tn10 and Tn5 insertions, respectively, into wild-type strain 14028. Fluorescein-labeled wild-type and mutant bacteria were incubated with host cells at various bacteria to cell ratios for 1 h at 37 degrees C and analyzed by flow cytometry. The mean fluorescence intensity of cells with associated wild-type and mutant bacteria was used to estimate the number of bacteria bound per host cell. Adherence was also measured by fluorescence microscopy. Neither assay showed a significant difference in binding of the ompC mutant and wild-type strains to the human cells. In contrast, the ompD mutant exhibited lowered binding to both cell types. Our findings suggest that OmpD but not OmpC is involved in the recognition of Salmonella serovar Typhimurium by human macrophages and intestinal epithelial cells.

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Section: Introductionmentioning

confidence: 99%

“…The Salmonella porin OmpC (Singh et al 2000) and the porin-like OmpA (Singh et al 2003) function as immunodominant antigens when mice are immunized with live or heat-killed Salmonella serovar Typhimurium. Salmonella porins have been proposed as broad-spectrum (Tabaraie et al 1994) or serovar-specific (Matsui and Arai 1989a) vaccines.…”

Section: Introductionmentioning

confidence: 99%

OmpD but not OmpC is involved in adherence ofSalmonella entericaserovar Typhimurium to human cells

Hara-Kaonga¹,

Pistole²

2004

Can. J. Microbiol.

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“…Since the mid-1980s, a major OMP of Salmonella has been the subject of much investigation, and our earlier work has shown that porins afford protection against Salmonella infection [6][7][8][9]. Several workers have demonstrated porins to be important antigens, which elicit CMI responses through a delayed-type hypersensitivity reaction, interleukin-2 (IL-2) and interferon-g (IFN-g) production, and mitogenic effects on T and B cells [4,10].…”

Section: Introductionmentioning

confidence: 99%

Priming of T‐Cell Responses in Mice by Porins of Salmonella typhimurium

Gupta

1998

Scand J Immunol

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An imbalance in signals delivered to T cells via T-cell receptor and accessory molecules can lead to anergy, apoptosis, or both. In the present study we have demonstrated that Salmonella typhimurium infection in mice leads to a progressive loss of CD4 þ T helper (Th) cell population, abnormal T-cell death by apoptosis and loss of accessory molecules (B7 and intracellular adhesion molecule-1) on macrophages. Quantification of interleukin-2 (IL-2), interleukin-4 (IL-4) and interferon-g (IFN-g) secretion revealed a Th2-type of response in lymphocytes isolated from spleen. However, preimmunization of mice with porins resulted in an increased CD4þ Th cell population and accessory molecules on the surface of macrophages. Quantification of cytokines revealed a Th1-type of response. We conclude that preimmunization of mice with porins provides a microenvironment in which a well-balanced accessory molecule and cytokine network is established, which results in the prevention of cell death by apoptosis.

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Macrophage‐T cell interaction in murine salmonellosis: selective down‐regulation of ICAM‐1 and B7 molecules in infected macrophages and its probable role in cell‐mediated immunity

et al. 1996

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Vaccine development and understanding of cellular immune modulatory mechanisms in salmonella infections have been impeded due to the paucity of data on antigens capable of eliciting effective immune responses. The present study was done to evaluate the efficacy of five major purified salmonella antigens (porins, pili, flagella, outer membrane proteins and heat shock proteins) in modulating T cell-macrophage interactions which play a central role in resistance to and recovery from infection with several intracellular pathogens, including salmonella. The results showed that the T cells recovered 10 days post-immunization (D10 T cells) from mice immunized with porins and outer membrane proteins showed maximum proliferation in the presence of macrophages incubated with dead bacteria; however, this response was decreased when T cells were co-cultured with live Salmonella typhimurium-infected macrophages. Delayed-type hypersensitivity responses, as measured by increased footpad thickness at 24 h, though induced effectively by porins, pili and flagella, were completely abrogated when D10 T cells were pre-incubated with macrophages infected with live bacteria. The phagocytic and bactericidal ability of normal macrophages, when grown in presence of T cell supernatants, was not influenced by the immunizing agents, but T cell supernatants obtained from mice immunized with porins and heat-shock protein triggered increased bactericidal activity. Further, the expression of the co-stimulatory molecules ICAM-1 and B7 increased with increasing bacteria (dead):macrophage ratio, but this expression was down-regulated upon incubation with live bacteria.

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Stimulation of Macrophage Oxygen Free Radical Production and Lymphocyte Blastogenic Response by Immunization with Porins

Abstract: Abstract:Porins were prepared from smooth strain of Salmonella typhi 0-901 and chemotype rough mutant of S. typhimurium Ra-30. Porins could significantly stimulate the immune systems of mice.

Cited by 8 publications

References 20 publications

OmpD but not OmpC is involved in adherence ofSalmonella entericaserovar Typhimurium to human cells

OmpD but not OmpC is involved in adherence ofSalmonella entericaserovar Typhimurium to human cells

Priming of T‐Cell Responses in Mice by Porins of Salmonella typhimurium

Macrophage‐T cell interaction in murine salmonellosis: selective down‐regulation of ICAM‐1 and B7 molecules in infected macrophages and its probable role in cell‐mediated immunity

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