Stimulation of Monocyte Procoagulant Activity by Adherence to Different Surfaces

Ginkel, C.J.W. van; Aken, W.G. van; Oh, Jaesung; Vreeken, J

doi:10.1111/j.1365-2141.1977.tb08761.x

Cited by 25 publications

(21 citation statements)

References 13 publications

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“…They suggested that, rather than express more TF, monocytes of patients with primary APS are primed by aPL to express more TF in the presence of a second stimulus. A major objection to this study is that adherence stimulates monocyte PCA (61). This may raise problems for testing differences in monocyte TF between patients and controls, since the PCA produced during separation and culture could mask the PCA that actually has these cells in blood circulation.…”

Section: Tf Pathway In Apsmentioning

confidence: 99%

The role of tissue factor in the antiphospholipid syndrome

Dobado‐Berrios

López-Pedrera

Velasco

et al. 2001

Arthritis & Rheumatism

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Section: Tf Pathway In Apsmentioning

confidence: 99%

The role of tissue factor in the antiphospholipid syndrome

Dobado‐Berrios

López-Pedrera

Velasco

et al. 2001

Arthritis & Rheumatism

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“…18,29 -31 However, culturing cells in suspension may represent a more natural state for peripheral blood monocytes and may influence cell susceptibility to a stimulus. [32][33][34] We therefore cultured monocytes from the same donor in 3 different ways: adhering to fibronectin-coated Petri dishes (Mo-FA; see Table 1A), adhering to plastic (Mo-A, unpublished data 2003), and in suspension on a Teflon membrane (Mo-S; see Table 1B). Although there were clear morphological differences between these differently cultured monocytes, none of the cultures expressed TF on stimulation by TF antigen (pg/10 6 cells) expression was measured by ELISA in cell lysates of (A) adherent monocytes (Mo-FA, nϭ11), (B) monocytes in suspension (Mo-S, nϭ4), or (C) macrophages (MDM, nϭ4) after 8 hours of stimulation with 100 g/mL CRP or 10 g/mL LPS.…”

Section: Influence Of Culture Conditionsmentioning

confidence: 99%

C-Reactive Protein Does Not Directly Induce Tissue Factor in Human Monocytes

Paffen

Vos

Bertina

2004

ATVB

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Objective-It is generally assumed that C-reactive protein (CRP) induces synthesis of tissue factor (TF) in monocytic cells, thereby potentially initiating intravascular blood coagulation. We aimed to elucidate the mechanism of CRP-induced TF expression in monocytes and monocyte-derived macrophages (MDMs) in vitro. Methods and Results-Monocytes were isolated from the blood of healthy donors and cultured with or without CRP or lipopolysaccharide (LPS) to study the time course of TF antigen and TF mRNA expression. Addition of 100 g/mL CRP did not result in a significant increase in TF antigen (range: 9 to 163 pg/10 6 cells, nϭ11) and TF mRNA (relative number of TF transcripts; N TF ϭ0.01 to 0.33), when compared with nonstimulated cells (TF antigen 7 to 46 pg/10 6 cells, N TF ϭ0.01 to 0.13). Variation of CRP concentration and exposure time did not affect the TF response. Similar results were obtained in monocytes cultured in suspension and in MDMs. In contrast, TF was strongly induced by 10 g/mL LPS (TF antigen 1125 to 6120 pg/10 6 cells, N TF ϭ5.94 to 23.43). Cultured monocytes did express FcR␥II, a putative CRP receptor, and addition of CRP induced a 7-fold increase in the production of monocyte chemoattractant protein-1 (MCP-1). Interestingly, CRP addition to peripheral blood mononuclear cells (PBMCs) did result in TF expression on monocytic cells. Conclusions-The

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“…The host response to infections, tumours or injury may be to activate the coagulation system within the circulation resulting in disseminated intravascular coagulation. Mononuclear phagocytes activate the blood coagulation process in response to bacterial endotoxin, immune complexes, complement components and other stimuli by synthesising and expressing tissue thromboplastin, which is the most potent activator of the extrinsic coagulation pathway (460,461). Moreover, it has been shown that mononuclear phagocytes from patients with meningococcal septicaemia (346).…”

Section: Blood Coagulationmentioning

confidence: 99%

Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions

Owen

Campbell

Stockley

1992

Journal of Leukocyte Biology

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Adherence of monocytes to extracellular matrix components is critical for their accumulation at sites of infection. To gain insight into the factors that regulate monocyte recruitment, we have studied monocyte adherence with regard to the regulatory effects of bacterial lipopolysaccharide (LPS) and the mechanisms involved; moreover, we have contrasted the phenotypes of adherent and nonadherent cells. Our results show that only a minor subpopulation of monocytes (20-25%) adhere spontaneously to fibronectin and that LPS stimulated a threefold increase in the proportion of adherent cells. Basal adherence and LPS-stimulated adherence of monocytes to fibronectin were substantially mediated by CD11/CD18 integrins. Further studies revealed that spontaneously adherent monocytes were 14-fold more actively phagocytic, released 1.6-fold more superoxide anion, and contained 20-fold more peroxidase activity than nonadherent cells, whereas LPS-adherent cells had an intermediate phenotype. These results indicate that LPS may enhance the accumulation of monocytes with an antimicrobial phenotype and thereby promote resolution of tissue infection.

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Stimulation of Monocyte Procoagulant Activity by Adherence to Different Surfaces

Cited by 25 publications

References 13 publications

The role of tissue factor in the antiphospholipid syndrome

The role of tissue factor in the antiphospholipid syndrome

C-Reactive Protein Does Not Directly Induce Tissue Factor in Human Monocytes

Monocyte adherence to fibronectin: role of CD11/CD18 integrins and relationship to other monocyte functions

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