Stimulation of neurite outgrowth in PC12D cells by ELF magnetic field and suppression by melatonin

Takatsuki, Hideyo; Yoshikoshi, Akio; Sakanishi, Akio

doi:10.1016/s0927-7765(02)00024-3

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…McFarlane et al showed that exposure to a 50 Hz AC EMF (4.35-8.25 µT) for 23 h during cell differentiation enhanced the NGF-induced neurite length in PC12 cells, whereas a slightly higher EMF (8.25-15.8 µT) did not [74]. A study by Takatsuki et al showed that an AC 60 Hz EMF (33.3 µT) increased neurite outgrowth in PC12D cells treated with forskolin [75]. Another research group found that an AC 60 Hz EMF (40 µT) [76] and a 50 Hz AC ELF-EMF (1 mT) for 5 days promoted NGF-induced differentiation in PC12 cells [77].…”

Section: Electromagnetic Field Stimulationmentioning

confidence: 99%

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

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PC12 cells, which are derived from rat adrenal pheochromocytoma cells, are widely used for the study of neuronal differentiation. NGF induces neuronal differentiation in PC12 cells by activating intracellular pathways via the TrkA receptor, which results in elongated neurites and neuron-like characteristics. Moreover, the differentiation requires both the ERK1/2 and p38 MAPK pathways. In addition to NGF, BMPs can also induce neuronal differentiation in PC12 cells. BMPs are part of the TGF-β cytokine superfamily and activate signaling pathways such as p38 MAPK and Smad. However, the brief lifespan of NGF and BMPs may limit their effectiveness in living organisms. Although PC12 cells are used to study the effects of various physical stimuli on neuronal differentiation, the development of new methods and an understanding of the molecular mechanisms are ongoing. In this comprehensive review, we discuss the induction of neuronal differentiation in PC12 cells without relying on NGF, which is already established for electrical, electromagnetic, and thermal stimulation but poses a challenge for mechanical, ultrasound, and light stimulation. Furthermore, the mechanisms underlying neuronal differentiation induced by physical stimuli remain largely unknown. Elucidating these mechanisms holds promise for developing new methods for neural regeneration and advancing neuroregenerative medical technologies using neural stem cells.

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Section: Electromagnetic Field Stimulationmentioning

confidence: 99%

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Tominami,

Kudo,

Noguchi

et al. 2024

IJMS

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“…In 1982, R. Dixey and G. Rein first reported the effects of pulsed EMF on 3 H-noradrenaline release from PC12 cells. Later many studies have found that exposure of the PC12 cell line or its subline to EMF can alter NGF induced neurite outgrowth [Blackman et al, 1993a[Blackman et al, ,b, 1995a[Blackman et al, ,b, 1996Mcfarlane et al, 2000;Shah et al, 2001;Takatsuki et al, 2002]. Nowadays, the PC12 cell line has served as a basic model for study of biological effect of EMF.…”

Section: Introductionmentioning

confidence: 99%

Influence of pulsed electromagnetic field with different pulse duty cycles on neurite outgrowth in PC12 rat pheochromocytoma cells

Zhang¹,

Ding²,

Duan³

et al. 2005

Bioelectromagnetics

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The influence of low frequency (50 Hz repetition rate) pulsed electromagnetic field (EMF) on PC12 cell neurite outgrowth in vitro was investigated in this study. We studied the percentage of neurite bearing cells, average length of neurites, and directivity of neurite outgrowth in PC12 cells cultured for 96 h in the presence of nerve growth factor (NGF). PC12 cells were exposed in one incubator to pulsed EMF at 1.36 mT (peak value) generated by a pair of Helmholtz coils, and the control samples were placed in another identical incubator. We found that the pulse duty cycle had significant effect on neurite outgrowth. Low (10%) pulse on-time significantly inhibited the percentage of neurite bearing cells, but at the same time increased the average length of neurites, while 100% on-time (DC) had exactly the opposite effects. Furthermore, we found that neurites were prone to extend along the direction of pulsed EMF with 10% pulse on-time. Our studies show that neurite outgrowth in PC12 cells is sensitive to the pulse duty and this sensitivity was associated with NGF concentration.

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“…The possible influence of EMF on neurite out-growth in PC12 cells has been investigated. Some results show that a weak electromagnetic field (mT) could promote the neurite outgrowth in PC12 cells [5,6], while inhibition of neurite out-growth was also observed [7]. One possible reason of the inconsistency of the published data is that the effect of EMF is strongly depended on the experimental condition, such as field strength, frequency of the pulsed field, orientation of electromagnetic field, exposure duration and NGF concentration.…”

mentioning

confidence: 99%

The influence of magnetic fields exposure on neurite outgrowth in PC12 rat pheochromocytoma cells

Fan

Ding

Duan

et al. 2004

Journal of Magnetism and Magnetic Materials

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Stimulation of neurite outgrowth in PC12D cells by ELF magnetic field and suppression by melatonin

Cited by 7 publications

References 29 publications

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review

Influence of pulsed electromagnetic field with different pulse duty cycles on neurite outgrowth in PC12 rat pheochromocytoma cells

The influence of magnetic fields exposure on neurite outgrowth in PC12 rat pheochromocytoma cells

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