Stimulation of plasma membrane h⁺-transport activity in barley roots by salt stress

The Effect of Air Pollution on Proline and Protein Content and Activity of Nitrate Reductase Enzyme in Laurus nobilis L. Plants

“…Azote is one of the most important elements in nutrition of plants (Neyra & Hangeman, 1975). When the azote content and its type is appropriate in nutrition of plant, formation of protein materials is increased, the livability of the plant is enhanced, leaf growth is accelerated and their ageing process is slowed down (Tatsumi, 1982) (Yamashita et al, 1994). In soil, azote is provided for roots of organic plants as nitrate and ammonium; although nitrate is the main source of azote (Baligar & Schanffert, 1993).…”

Salt stress-induced enhancement of anion efflux and anion transport activity in plasma membrane of barley roots

“…Results and discussion We previously demonstrated that the plasma membrane ATPase activity decreased after treatment with NaC1 at a concentration over 200 mol m -3 for 1 d (Yamashita et al 1994). The reduction of the ATPase activity by salt stress was caused by the decrease in the amount of H+-ATPase rather than by the alteration of the lipid environment (Yamashita and Matsumoto, in preparation).…”

“…The reduction of the ATPase activity by salt stress was caused by the decrease in the amount of H+-ATPase rather than by the alteration of the lipid environment (Yamashita and Matsumoto, in preparation). The early response of barley roots to NaC1 stress was ascribed to the salt stress-induced stimulation of chloride permeability of plasma membrane, which was estimated by the effect of anions on H+-transport activity (Yamashita et al 1994). Therefore, in this study, we determined directly the effect of salt stress on the permeability of plasma membrane vesicles to chloride using a6C1-as a radio tracer.…”

“…The plasma membrane vesicles were purified from microsomes by the aqueous polymer two-pase system (Yamashita et al 1994).…”

Evidence for Nucleotide-Dependent Passive H+ Transport Protein in the Plasma Membrane of Barley Roots

“…+ transport by the octylglucoside soluble fraction Plasma membranes were isolated with high purity from the microsomal fractions of barley roots by an aqueous polymer two-phase partitioning technique (Yamashita et al 1994). We treated the plasma membranes with octylglucoside at 1%, and obtained octylglucoside-soluble and -insoluble fractions.…”