Stimulation of Rat Gastric Adenylate Cyclase by Histamine and Histamine Analogues and Blockade by Burimamide

Abstract

Histamine, 4‐methylhistamine, 3‐(β‐aminoethyl)‐1,2,4 triazole and betazole, in that order, stimulated adenylate cyclase prepared from rat gastric tissue in a dose‐dependent manner. Burimamide, an H2‐receptor blocking agent, in concentrations of 1–5 × 10−6 m antagonized this effect. The data lend some support to the hypothesis that elevated levels of cyclic adenosine 3′,5′‐monophosphate may be involved in histamine‐stimulated gastric secretions and that H2‐receptors are associated with adenylate cyclase.

Evidence for involvement of cyclic nucleotides in intrinsic factor secretion by isolated rabbit gastric mucosa.

“…Since Kimberg's comprehensive review (1) appeared in 1974, the results of numerous investigations have argued for (2)(3)(4)(5)(6)(7) and against (8,9) an important role for cyclic nucleotides in mammalian gastric secretion. These investigations have been concerned with hydrochloric acid secretion (6,8) and with adenylate cyclase activity and levels of adenosine 3,5'-cyclic AMP (cAMP)l in gastric mucosa (2)(3)(4)(5)(7)(8)(9).…”

“…These investigations have been concerned with hydrochloric acid secretion (6,8) and with adenylate cyclase activity and levels of adenosine 3,5'-cyclic AMP (cAMP)l in gastric mucosa (2)(3)(4)(5)(7)(8)(9). We are unaware of studies on the possible relationships between cyclic nucleotides and gastric secretion of macromolecules.…”

Interaction of prostaglandins and histamine with enzymes of cyclic AMP metabolism from guinea pig gastric mucosa.

“…A number of studies in mammalian as well as in nonmammalian species indicate that histamine's action on oxyntic cells is mediated at least in some species through stimulation of cyclic AMP formation. The evidence for such mechanism is based mainly on observations that histamine stimulates adenylate cyclase in cellfree preparations (4)(5)(6)(7)(8)(9)(10)(11)(12) and also causes the accumulation of cyclic AMP in unbroken cell preparations (tissue slices) of fundic gastric mucosa (13)(14)(15), concomitant with in situ activation of cyclic AMP-dependent protein kinase (15). Stimulation of gastric mucosa cyclic AMP production by histamine is probably due to its interaction with the H2 receptors since specific histamine H2-receptor antagonists inhibit the effect of histamine on both cyclic kMP formation and HCl secretion (11,12,(16)(17)(18).…”

Effect of Actinomycin D on Pentagastrin- and Histamine-Stimulated Secretion from Isolated Canine Stomachs

“…In some species, cyclic AMP appears to be involved. Histamine was found to stimulate gastric mucosal adenyl cyclase in the rat (5,19), rabbit (20), and guinea pig (21). The hypothesis that the adenyl cyclase-cyclic AMP system also mediates histamine-stimulated acid secretion has been verified in amphibians (22,23), rats (4,5,13) and humans (24,25).…”