Stimulation of respiratory burst and phagocytic activity in Atlantic salmon (Salmo salar L.) macrophages by lipopolysaccharide

Solem, Stein Tore; Jørgensen, Jorunn B.; Robertsen, Børre

doi:10.1016/s1050-4648(95)80049-2

Cited by 121 publications

(78 citation statements)

References 30 publications

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“…5. Sensitivity of both methods has been shown by Solem et al (1995) who demonstrated a dose-dependent stimulation of phagocytosis using different concentrations of LPS.…”

Section: Phagocytosismentioning

confidence: 99%

“…1) (Ainsworth, 1992;Secombes and Fletcher, 1992;Dannevig et al, 1994). However, it has also been shown that the activation of monocytes by LPS or opsonization of microorganisms through complement components or antibodies leads to increased phagocytosis (Gudmundsdottir et al, 1995;Solem et al, 1995).…”

Section: Phagocytosismentioning

confidence: 99%

“…A method to determine respiratory burst. After in vivo stimulation leukocytes are cultivated with different bacteria to induce respiratory burst (Solem et al, 1995). Note the increased response of the in vivo primed leukocytes after in vitro restimulation with A. salmonicida.…”

Section: Phagocytosismentioning

confidence: 99%

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Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

et al. 2002

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In fish, the first line of defense against infectious microorganisms is based on a broad range of nonspecific humoral and cellular immune mechanisms ('innate immunity') which without prior specific activation can act in forming a more static barrier (Fish Shellfish Immunol. 10 (2000) 243; Dev. Comp. Immunol. 25 (2001) 827). This natural resistance is normally effective enough to protect fish from infectious diseases until specific immune responses are being induced ( Fig. 1; Dev. Comp. Immunol. 25 (2001) 841). Healthy fish exhibit both nonspecific and specific immune responses depending directly on environmental temperature. Pollution of the natural aquatic environment with industrial or agricultural sewage is an important immunosuppressing factor resulting in higher susceptibility to infectious diseases. To date, the possible immunotoxicity of a substance is evaluated using quantification of humoral factors like lysozyme, complement, C-reactive protein or total immunoglobulins but less often using functional assays. Furthermore, most of the functional assays (phagocytosis, respiratory burst, proliferative response) are based on the measurement of the response of resting but not of specific activated immune cells. However, the physiological responses of the immune system to an infection are based on a complex, stepwise activation and proliferation, especially of the specific immune functions after first contact to the microorganisms. In this report we describe in vitro methods for the evaluation of cellular immune functions of different leukocyte populations after specific in vivo triggering of the immune system. Parameters to be evaluated are activation and proliferation of leukocyte populations, phagocytosis and respiratory burst, secretion of antigen-specific antibodies and specific cell-mediated cytotoxicity. Furthermore, challenge models with bacterial (Aeromonas salmonicida) and viral pathogens (Viral Haemorrhagic Septicemia Virus, VHSV) are presented.

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“…5. Sensitivity of both methods has been shown by Solem et al (1995) who demonstrated a dose-dependent stimulation of phagocytosis using different concentrations of LPS.…”

Section: Phagocytosismentioning

confidence: 99%

Section: Phagocytosismentioning

confidence: 99%

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Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

et al. 2002

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“…动物学研究 33 卷 bicoloratus) (Shen et al, 2001) 、鳗鲡 (Anguilla anguilla) (Xu et al, 2005) 和军曹鱼 (Rachycentron canadum) (Jian et al, 2003)等鱼类。目前, 国内外学者大量报道了弧菌等致病菌灭活疫苗对水产养殖动物免疫应答方面的研究 (Kawai & Kusuda, 1995;Ma et al, 2010)。然而, 灭活疫苗中包含诸多无效抗原, 所产生的抗体并不是全部具有保护性。脂多糖(lipopolysaccaride, LPS)和外膜蛋白(outer membrane protein, OMP)都是病原菌的菌体成分, 是主要保护性抗原 (Yan, 2005)。许多研究结果表明, 脂多糖(lipopolysaccaride, LPS)和外膜蛋白(outer membrane protein, OMP)皆具有较强的免疫原性, 可以提高机体的非特异性免疫功能 (Dalmo et al, 2000;Huang & Lu, 2002;Bader et al, 2004;Yan, 2005); LPS 能刺激 B 淋巴细胞产生特异性抗体, 引起机体的免疫反应; OMP 可激发机体的体液免疫和细胞免疫, 并且可通过遗传工程大量表达 (Bøgwald et al, 1992;Solem et al, 1995;Rahman & Kawai, 2000;Mo et al, 2002) (Ma et al, 2008;Yan et al, 2010) …”

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Effects of three antigens extracted from <I>Vibrio vulnificus</I> on the immunological protection of <I>Nibea albiflora</I>

Yan¹,

Shan²,

Chen³

et al. 2013

Zoological Research

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“…Niejednokrotnie wykazano, że eksperymentalne podanie LPS u zwierząt doświadczalnych (myszy i szczurów), a także immunizacja pstrągów przeciwko jersiniozie i wrzodzienicy indukują stres oksydacyjny (1,3,32,33). Dotychczasowe badania u ryb wykazały silne działanie immunostymulujące lipopolisacharydu manifestujące się między innymi wzrostem aktywności metabolicznej komórek fagocytujących wskazujących na wystąpienie "wybuchu tlenowego" (10,25,28).…”

Section: Praca Oryginalnaunclassified

Effect of lipopolysaccharide (LPS) on the antioxidant enzyme system activity of erythrocytes and phagocytes of carps in in vivo studies

Sopińska¹,

Grochoła²

2018

Medycyna Weterynaryjna

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The purpose of this experiment was to estimate the activity of antioxidant enzymes (SOD, GPx, CAT) in erythrocytes and phagocytes of carps (Cyprinus carpio L.) after intraperitoneal injection of LPS at a dose of 75 μg/100 g b.w. Enzyme activity was determined 3, 6 and 12 hours, as well as 3, 7, 14 and 28 days after LPS administration. After 3 and 6 hours, SOD in erythrocytes increased, respectively, to 188% and 142% of its control group level, and after 12 hours, SOD activity was significantly reduced (117%) and remained unchanged until the end of the experiment. From 12 hours after LPS administration until the end of the study, the PGx level was statistically significantly lower than in the control group, whereas the catalase activity was statistically significantly lower than in the control group in all study periods. In kidney phagocytic cells, SOD activity after 12 hours and 3 days was similar to that in the control group, and in the following study periods it amounted to 66-78% of the control values. Until the 14th day of observation, PGx activity was statistically significantly lower than it was in the control group. Catalase activity in kidney leukocytes was statistically significantly lower than in the control group during the entire experiment, and the lowest in the first study days, amounting to 48-42% of the control group value. The results indicate a long-term decrease in antioxidant enzyme activities in the experimental fish (lasting 14 or 28 days)...

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Stimulation of respiratory burst and phagocytic activity in Atlantic salmon (Salmo salar L.) macrophages by lipopolysaccharide

Cited by 121 publications

References 30 publications

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

Evaluation of immune functions of rainbow trout (Oncorhynchus mykiss)—how can environmental influences be detected?

Effects of three antigens extracted from <I>Vibrio vulnificus</I> on the immunological protection of <I>Nibea albiflora</I>

Effect of lipopolysaccharide (LPS) on the antioxidant enzyme system activity of erythrocytes and phagocytes of carps in in vivo studies

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